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        Genome-wide identification and characterization of the RIO atypical kinase family in plants

        Qingsong Gao,Shuhui Xu,Xiayuan Zhu,Lingling Wang,Zefeng Yang,Xiangxiang Zhao 한국유전학회 2018 Genes & Genomics Vol.40 No.6

        Members of the right open reading frame (RIO) atypical kinase family are present in all three domains of life. In eukaryotes, three subfamilies have been identified: RIO1, RIO2, and RIO3. Studies have shown that the yeast and human RIO1 and RIO2 kinases are essential for the biogenesis of small ribosomal subunits. Thus far, RIO3 has been found only in multicellular eukaryotes. In this study, we systematically identified members of the RIO gene family in 37 species representing the major evolutionary lineages in Viridiplantae. A total of 84 RIO genes were identified; among them, 41 were classified as RIO1 and 43 as RIO2. However, no RIO3 gene was found in any of the species examined. Phylogenetic trees constructed for plant RIO1 and RIO2 proteins were generally congruent with the species phylogeny. Subcellular localization analyses showed that the plant RIO proteins were localized mainly in the nucleus and/or cytoplasm. Expression profile analysis of rice, maize, and Arabidopsis RIO genes in different tissues revealed similar expression patterns between RIO1 and RIO2 genes, and their expression levels were high in certain tissues. In addition, the expressions of plant RIO genes were regulated by two drugs: mycophenolic acid and actinomycin D. Function prediction using genome-wide coexpression analysis revealed that most plant RIO genes may be involved in ribosome biogenesis. Our results will be useful for the evolutionary analysis of the ancient RIO kinase family and provide a basis for further functional characterization of RIO genes in plants.

      • Research on the Development of University Course Ideological and Political Collaboration under the Background of the Construction of Double City Economic Circle

        况,迎春(Kuang Yingchun),高雅(Gao Ya),李青,嵩(Li Qingsong) 아시아사회과학학회 2021 International Science Research Vol.1 No.1

        Under the background of Chengdu Chongqing double city economic circle, through the analysis of the current situation and problems of the ideological and political construction and development of Chengdu Chongqing universities, combined with the advantages of Sichuan and Chongqing universities, this paper seeks measures to improve the ideological and political construction and development of Chengdu Chongqing universities. It is found that the ideological and political construction and development of colleges and universities in Chengdu and Chongqing can be improved mainly by strengthening the ideological and political education model and reforming and innovating, integrating and optimizing relevant educational resources; Specific measures include integrating online media, strengthening practical teaching, optimizing teachers and improving infrastructure construction.

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        3.2-kW 9.7-GHz Polarization-maintaining Narrow-linewidth All-fiber Amplifier

        Hang Liu,Yujun Feng,Xiaobo Yang,Yao Wang,Hongming Yu,Jue Wang,Wanjing Peng,Yanshan Wang,Yinhong Sun,Yi Ma,Qingsong Gao,Chun Tang 한국광학회 2024 Current Optics and Photonics Vol.8 No.1

        We present a Yb-doped narrow-linewidth polarization-maintaining (PM) all-fiber amplifier that achieves a high mode-instability (MI) threshold, high output power, and 9.7-GHz spectral linewidth. Six wavelength-multiplexed laser diodes are used to pump this amplifier. First, we construct a high-power fiber amplifier based on a master oscillator-power amplifier (MOPA) configuration for experiments. Subsequently, we examine the MI threshold by individually pumping the amplifier with wavelengths of 976, 974, 981, 974, and 981 nm respectively. The experimental results demonstrate that the amplifier exhibits a high MI threshold (>3.5 kW) when pumped with a combination of wavelengths at 974 and 981 nm. Afterward, we inject an optimized phase-modulated seed with a nearly flat-top spectrum into this amplifier. Ultimately, laser output of 3.2 kW and 9.7 GHz are obtained.

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