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      저자 : Qinghong Yin (검색결과 2 건)
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        WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling

        Sun Chao,Ma Qinghong,Yin Jian,Zhang Han,Liu Xinhui 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

        Ongoing chronic fibrosis and hypertrophy of the ligamentum flavum (LF) is an important cause of lumbar spinal canal stenosis (LSCS). Our previous work showed that WNT1-inducible signaling pathway protein 1 (WISP-1) is a critical driver of LF fibrosis. However, the potential mechanism has not been explored. Here, we found that Gli1 was upregulated in hypertrophic LF tissues and required for fibrogenesis in fibroblasts. Moreover, mechanical stretching increased the expression of WISP-1 in LF fibroblasts. Furthermore, WISP-1 induced fibrogenesis in vitro through the Hedgehog-Gli1 pathway. This conclusion was supported by the fact that WISP-1 activated the Hedgehog-Gli1 pathway in LF fibroblasts and that cyclopamine attenuated the effect of WISP-1-induced fibrogenesis. WISP-1 also promoted the transition of fibroblasts into myofibroblasts via the Hedgehog pathway. Importantly, a hypertrophic LF rabbit model induced by mechanical stress also showed pathological changes in fibrosis and elevated expression of WISP-1, Gli1, and α-SMA. Therapeutic administration of cyclopamine reduced collagen expression, fibroblast proliferation, and myofibroblast differentiation and ameliorated fibrosis in the mechanical stress-induced rabbit model. Collectively, our findings show mechanical stress/WISP-1/Hedgehog signaling as a new fibrotic axis contributing to LF hypertrophy and identify Hedgehog signaling as a therapeutic target for the prevention and treatment of LF fibrosis.

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        Metastable equilibrium for the quaternary system containing with lithium+ potassium+magnesium+chloride in aqueous solution at 323K

        Xudong Yu,Ying Zeng,Qinghong Yin,Dongbo Jiang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.6

        The metastable equilibrium of the system contained with lithium, potassium, magnesium, and chloridein aqueous system was investigated at 323 K using an isothermal evaporation method. The isothermal experimentaldata and physicochemical properties, such as density and refractive index of the equilibrated solution, were determined. With the experimental results, the stereo phase diagram, the projected phase diagram, the water content diagram andthe physicochemical properties versus composition diagrams were constructed. The projected phase diagram consistsof three invariant points, seven univariant curves and five crystallization fields corresponding to single salts potassiumchloride (KCl), lithium chloride monohydrate (LiCl·H2O), bischofite (MgCl2·6H2O) and two double salts lithium carnallite(LiCl·MgCl2·7H2O) and potassium carnallite (KCl·MgCl2·6H2O). Salt KCl has the largest crystallization region;it contains almost 95% of the general crystallization field.

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