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RISS 인기검색어
- 검색키워드
- 저자 : Qinggang Chen (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.
Chen, Keping,Zhang, Chunxia,Yao, Qin,Xu, Qinggang,Tang, Xudong Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.2
Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fern gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely ${OPG-07_496}, {OPC-15_1,660} and {OPE-18_1,279}$. Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from ${OPG-07_496} and {OPC-15_1,660}$ are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).
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Vitellogenin’s putative role in Tegillarca granosa’s cadmium detoxification
Caifang Chen,Weiliang Shen,Hailong Gu,Linde Wu,Zhihua Lin,Qinggang Xue 한국유전학회 2017 Genes & Genomics Vol.39 No.2
The bloody clam, Tegillarca granosa, is a commercial benthic bivalve, having a strong accumulation ability and torrelence to cadmium. To investigate whether vitellogenin (Vg) is involved in cadmium (Cd) detoxification, the full-length cDNA of T. granosa Vg was cloned, and its expression pattern in response to cadmium exposure was studied compared with the reference metallothionein (MT) gene. The full T. granosa Vg sequence consisted of 8988 bp, including a 6930-bp open reading frame that encoded a 2309 amino acid polypeptide. The deduced Vg protein contained a Vg N-terminal domain, domain of unknown function (DUF1943), SbcC domain, and von Willebrand factor typeD domain (VWD). Multiple metal-binding sites were predicted in the deduced T. granosaVg protein, suggesting its potential in functioning as a metal-binding protein. In addition, Vg expression increased in the T. granosa digestive gland and hemolymph in time-dependent manner after exposure to 1, 3, 6 and 9 lg/L Cd for 28 days.MTexpression was measured in parallel with Vg expression, and the latter was more sensitive to Cd induction than the former. Together, results of the present research suggested that Vg may play an important role in T. granosa metal detoxification.
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Ying Cui,Qinggang Chen,Yaxiao Li,Ling Tang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.2
Flavonoids exhibit a high affinity for the purifiedcytosolic NBD (C-terminal nucleotide-binding domain) ofP-glycoprotein (P-gp). To explore the affinity of flavonoidsfor P-gp, quantitative structure–activity relationship(QSAR) models were developed using support vectormachines (SVMs). A novel method coupling a modifiedparticle swarm optimization algorithm with randommutation strategy and a genetic algorithm coupled withSVM was proposed to simultaneously optimize the kernelparameters of SVM and determine the subset of optimizedfeatures for the first time. Using DRAGON descriptors torepresent compounds for QSAR, three subsets (training,prediction and external validation set) derived from thedataset were employed to investigate QSAR. Withexcluding of the outlier, the correlation coefficient (R2) ofthe whole training set (training and prediction) was 0.924,and the R2 of the external validation set was 0.941. Theroot-mean-square error (RMSE) of the whole training setwas 0.0588; the RMSE of the cross-validation of theexternal validation set was 0.0443. The mean Q2 value ofleave-many-out cross-validation was 0.824. With moreinformations from results of randomization analysis andapplicability domain, the proposed model is of good predictiveability, stability.
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Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.
Chunxia Zhang,Qin Yao,Qinggang Xu,Xudong Tang,Keping Chen 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2
Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fem gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely OPG-07496, OPC-151,660 and OPE-181,279. Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from OPG-07496 and OPC-151,660 are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).
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