Reclassification of Bacillus beijingensis Qiu et al. 2009 and Bacillus ginsengi Qiu et al. 2009 as Bhargavaea beijingensis comb. nov. and Bhargavaea ginsengi comb. nov. and emended description of the genus Bhargavaea

Verma, Pankaj,Pandey, Prashant Kumar,Gupta, Arvind Kumar,Seong, Chi Nam,Park, Seong Chan,Choe, Han Na,Baik, Keun Sik,Patole, Milind Shivaji,Shouche, Yogesh Shreepad Microbiology Society 2012 International journal of systematic and evolutiona Vol.62 No.10

<P>We have carried out a polyphasic taxonomic characterization of <I>Bacillus beijingensis</I> DSM 19037<SUP>T</SUP> and <I>Bacillus ginsengi</I> DSM 19038<SUP>T</SUP>, which are closely related phylogenetically to <I>Bhargavaea cecembensis</I> LMG 24411<SUP>T</SUP>. All three strains are Gram-stain-positive, non-motile, moderately halotolerant and non-spore-forming. 16S rRNA gene sequence analyses showed that the strains constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. The percentage similarity on the basis of amino acid sequences deduced from partial <I>gyrB</I> gene nucleotide sequences of these three type strains was 96.1-92.7 %. Phylogenetic trees based on the 16S rRNA gene and GyrB amino acid sequences, obtained by using three different algorithms, were consistent and showed that these three species constituted a deeply rooted cluster separated from the clades represented by the genera <I>Bacillus</I>, <I>Planococcus</I>, <I>Planomicrobium</I>, <I>Sporosarcina</I>, <I>Lysinibacillus</I>, <I>Viridibacillus</I>, <I>Kurthia</I> and <I>Geobacillus</I>, supporting their placement in the genus <I>Bhargavaea</I>. All three type strains have menaquinone MK-8 as the major respiratory quinone and showed similar fatty acid profiles. The main polar lipids present in the three type strains were diphosphatidylglycerol and phosphatidylglycerol, and the three strains showed peptidoglycan type A4α with l-lysine as the diagnostic diamino acid. The DNA G<I>+</I>C contents of <I>Bacillus beijingensis</I> DSM 19037<SUP>T</SUP>, <I>Bacillus ginsengi</I> DSM 19038<SUP>T</SUP> and <I>Bhargavaea cecembensis</I> LMG 24411<SUP>T</SUP> were 53.1, 50.2 and 53.7 mol%, respectively. The level of DNA-DNA hybridization among the three strains was 57-39 %, indicating that they are members of different species of the genus <I>Bhargavaea</I>. The phenotypic data are consistent with the placement of these three species in a single genus and support their differentiation at the species level. On the basis of these data, we have emended the description of the genus <I>Bhargavaea</I> and propose the reclassification of <I>Bacillus beijingensis</I> and <I>Bacillus ginsengi</I> to the genus <I>Bhargavaea</I>, as <I>Bhargavaea beijingensis</I> comb. nov. (type strain ge10<SUP>T</SUP> = DSM 19037<SUP>T</SUP> = CGMCC 1.6762<SUP>T</SUP>) and <I>Bhargavaea ginsengi</I> comb. nov. (type strain ge14<SUP>T</SUP> = DSM 19038<SUP>T</SUP> = CGMCC 1.6763<SUP>T</SUP>).</P>

Bhargavaea indica sp. nov., a Member of the Phylum Firmicutes, Isolated from Arabian Sea Sediment

Pankaj Verma,성치남,Prashant Kumar Pandey,Ramesh Ramchandra Bhonde,Cathrin Spröer,Manfred Rohde,Yogesh Shreepad Shouche 한국미생물학회 2013 The journal of microbiology Vol.51 No.1

A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, nonendospore-forming and grew with 0–8.5% (w/v) NaCl, at 15–48°C and at pH 6.5–9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98T forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA–DNA relatedness values of strain KJW98T with B. beijingensis DSM 19037T, B. cecembensis LMG 24411T and B. ginsengi DSM 19038T were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 (37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA–DNA relatedness data indicate that strain KJW98T should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98T (=KCTC 13583T=LMG 25219T).

Periodontal healing following non-surgical repair of an old perforation with pocket formation and oral communication

Ali Nosrat,Prashant Verma,Saeed Asgary 대한치과보존학회 2018 Restorative Dentistry & Endodontics Vol.43 No.2

Iatrogenic perforations negatively impact the outcome of endodontic treatments. Studies on prognostic factors showed that perforations in the coronal third of the root with periodontal pocket formation have an unfavorable prognosis. A 36-year-old female was referred for endodontic evaluation of tooth #13 with a history of an iatrogenic perforation, happened 3 years ago. There was a sinus tract associated with perforation, 10 mm probing on the mesial and mesio-palatal, bleeding on probing, radiolucent lesion adjacent to the perforation and complete resorption of the interdental bone between teeth #13 and #12. After the treatment options were discussed, she chose to save the tooth. The tooth was accessed under rubber dam isolation, the perforation site was cleaned and disinfected using 0.5% sodium hypochlorite and sealed with calcium-enriched mixture cement. Eighteen months after treatment the tooth was functional and asymptomatic. The probing depths were normal without bleeding on probing. Radiographically, the interdental crestal bone formed between teeth #13 and #12. Despite all negative prognostic factors in this case (i.e., perforations in the coronal third, pocket formation, and radiolucent lesion), healing was unexpectedly achieved via non-surgical repair of the perforation. Further research on biological aspects of healing in the periodontium following iatrogenic perforations are recommended.

Periodontal healing following non-surgical repair of an old perforation with pocket formation and oral communication

Asgary, Saeed,Verma, Prashant,Nosrat, Ali The Korean Academy of Conservative Dentistry 2018 Restorative Dentistry & Endodontics Vol.43 No.2

Iatrogenic perforations negatively impact the outcome of endodontic treatments. Studies on prognostic factors showed that perforations in the coronal third of the root with periodontal pocket formation have an unfavorable prognosis. A 36-year-old female was referred for endodontic evaluation of tooth #13 with a history of an iatrogenic perforation, happened 3 years ago. There was a sinus tract associated with perforation, 10 mm probing on the mesial and mesio-palatal, bleeding on probing, radiolucent lesion adjacent to the perforation and complete resorption of the interdental bone between teeth #13 and #12. After the treatment options were discussed, she chose to save the tooth. The tooth was accessed under rubber dam isolation, the perforation site was cleaned and disinfected using 0.5% sodium hypochlorite and sealed with calcium-enriched mixture cement. Eighteen months after treatment the tooth was functional and asymptomatic. The probing depths were normal without bleeding on probing. Radiographically, the interdental crestal bone formed between teeth #13 and #12. Despite all negative prognostic factors in this case (i.e., perforations in the coronal third, pocket formation, and radiolucent lesion), healing was unexpectedly achieved via non-surgical repair of the perforation. Further research on biological aspects of healing in the periodontium following iatrogenic perforations are recommended.

Morin ameliorates myocardial injury in diabetic rats via modulation of inflammatory pathways

Vipin Kumar Verma,Salma Malik,Ekta Mutneja,Anil Kumar Sahu,Vaishali Prajapati,Prashant Mishra,Jagriti Bhatia,Dharamveer Singh Arya 한국실험동물학회 2024 Laboratory Animal Research Vol.40 No.1

Background: High blood glucose levels in diabetes lead to vascular inflammation which accelerates atherosclerosis. Herein, Morin was orally administered in male Wistar rats, at the dose of 40 mg/kg for 28 days, and on the 27th and 28th day, ISO was administered to designate groups at the dose of 85 mg/kg s.c., to induce myocardial infarction. Results: Free radical generation, including ROS, in diabetes following ISO administration, leads to the activation of both intrinsic and extrinsic pathways of apoptosis. Morin significantly (p ≤ 0.05) reduced oxidative stress (GSH, MDA, SOD), cardiac injury markers (CK-MB, LDH), inflammation (TNF, IL-6), and apoptosis (Bax, BCl2, Caspase-3). In addition, it also reduced insulin and blood glucose levels. Akt/eNOS, Nrf2/HO-1, MAPK signaling pathways, and Insulin signal transduction pathways were positively modulated by morin pre-treatment. Conclusions: Morin attenuated oxidative stress and inflammation and also modified the activity of various molecular pathways to mitigate cardiomyocyte damage during ISO-induced MI in diabetic rats.

3(2) Designing and optimization of aceclofenac transdermal films using response surface methodology: investigating the effect of hydrophilic and hydrophobic matrix on ex vivo and in vivo permeation characteristics

Pragya Yadav,Vaibhav Rastogi,Mayur Porwal,Prashant Upadhyay,Anurag Verma 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.6

3(2) factorial design and response surface methodology in the fabrication of matrix transdermal films of aceclofenac (ACL) was utilized in this study to investigate the effects of polymers (hydrophilic and hydrophobic) and penetration enhancer (PE) on ex vivo and in vivo drug performance. FTIR and DSC showed no interaction of drug with polymers. The physicochemical characterization showed uniformity among the formulations. Results from the permeation study suggested the effect of polyvinyl pyrrolidone (PVP K-30) and PE, on increasing concentration of PVP K-30 with respect to ethyl cellulose as well as increased concentration of PE resulted in enhanced permeation through the matrix. Significant effect of PE on the lipid and protein framework of the skin was also observed which is responsible for increased permeation. From response surface curve and contour plots, an optimized formulation was selected and evaluated for their ex vivo permeation, skin irritation, in vivo efficacy and stability studies. The optimized formulation showed Higuchi controlled diffusion and was found to be non-irritant and efficacious in reducing carrageenan induced paw edema in rats over a prolonged duration of time as compared to oral delivery. Thus, results indicated that the prepared transdermal system can be a promising non-oral approach to deliver effective amount of ACL to treat various inflammatory conditions.

Prevalence of Irritable Bowel Syndrome: A Community Based Study From Northern India

( Govind K Makharia ),( Anil K Verma ),( Ritvik Amarchand ),( Anil Goswami ),( Prashant Singh ),( Abhishek Agnihotri ),( Faizul Suhail ),( Anand Krishnan ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2011 Journal of Neurogastroenterology and Motility (JNM Vol.17 No.1

Background/Aims The prevalence of irritable bowel syndrome (IBS) varies from 4% to 20% in different Asian nations. Prevalence of IBS in native North Indian community is not known. Methods Between November 2008 to December 2009, we estimated the prevalence of IBS in a rural community of Ballabgarh block, located in Haryana state. A structured questionnaire based on Rome Ⅲ module was used to collect symptoms related to IBS from all the participants in a door to door survey. A Rome Ⅲ criterion was used for diagnosis of IBS. IBS was further classified based on predominance of symptoms as constipation predominant, diarrhea predominant, mixed and unspecified based on Rome Ⅲ module. Results There were 4,767 participants (mean age 34.6±10.8, males 50%). Overall, 555 (11.6%; 95% CI, 10.7-12.5) had constipation, 542 (11.4%; 95% CI, 10.5-12.3) diarrhea and 823 (17.3%; 95% CI, 16.2-18.4) abdominal pain. The overall prevalence of IBS was 4% (95% CI, 3.5-4.6). The prevalence of constipation predominant IBS was 0.3% (95% CI, 0.16-0.49), diarrhea predominant IBS 1.5% (95% CI, 1.18-1.90), mixed IBS 1.7% (95% CI, 1.35-2.11) and unsubtyped IBS 0.5% (95% CI, 0.32-0.75). The prevalence of IBS was significantly higher in females compared with males (4.8% vs 3.2%, P=0.008). However, there was no significant difference between males and females in the prevalence of different subtypes of IBS. The prevalence increased with age. Conclusions The prevalence of IBS in a North Indian community is 4%. IBS poses a significant burden on the rural adults. (J Neurogastroenterol Motil 2011;17:82-87)

Generalized anxiety and sleep quality among health care professionals during the COVID-19 pandemic: a cross-sectional study from a tertiary healthcare institution in Eastern India

Bijaya Nanda Naik,Sanjay Pandey,Rajath Rao,Manisha Verma,Prashant Kumar Singh 질병관리본부 2022 Osong Public Health and Research Persptectives Vol.13 No.1

Objectives: With the emergence of the coronavirus disease 2019 (COVID-19) pandemic, healthcare professionals (HCPs) have experienced high levels of stress and anxiety because of the high risk of infection for themselves and their families. This has led to acute sleep problems for HCP. This study was designed to assess the anxiety and sleep quality of HCPs during the COVID-19 pandemic. Methods: This cross-sectional study analyzed 370 HCPs employed at All India Institute of Medical Sciences Patna over 3 months, using the standard Generalized Anxiety Disorder 7-item scale (GAD-7) for suspected GAD and the Pittsburgh Sleep Quality Index for sleep quality. Results were tabulated and multivariable binomial logistic regression analysis was done to determine the predictors of poor sleep. Significance was attributed to p < 0.05. Results: Of the 370 HCPs screened, 52 (14.1%; 95% confidence interval [CI], 10.8%–18.1%) were found to have GAD and 195 (52.7%; 95% CI, 47.5%–57.9%) were found to be poor sleepers. The presence of any addictive habit (adjusted odds ratio [AOR], 1.833; 95% CI, 1.12–2.8), unprotected contact with COVID-19 cases (AOR, 1.902; 95% CI, 1.1–3.3), and the presence of GAD (AOR, 5.57; 95% CI, 2.5–12.4) were found to be predictors of poor sleep quality among HCPs. Conclusion: A significant proportion of HCPs were found to have suspected GAD and were poor sleepers. This highlights the need for measures to confront this problem.

Quantitative histology-based classification system for assessment of the intestinal mucosal histological changes in patients with celiac disease

Prasenjit Das,Gaurav PS Gahlot,Alka Singh,Vandana Baloda,Ramakant Rawat,Anil K Verma,Gaurav Khanna,Maitrayee Roy,Archana George,Ashok Singh,Aasma Nalwa,Prashant Ramteke,Rajni Yadav,Vineet Ahuja,Vishnu 대한장연구학회 2019 Intestinal Research Vol.17 No.3

Background/Aims: The existing histological classifications for the interpretation of small intestinal biopsies are based on qualitative parameters with high intraobserver and interobserver variations. We have developed and propose a quantitative histological classification system for the assessment of intestinal mucosal biopsies. Methods: We performed a computer-assisted quantitative histological assessment of digital images of duodenal biopsies from 137 controls and 124 patients with celiac disease (CeD) (derivation cohort). From the receiver-operating curve analysis, followed by multivariate and logistic regression analyses, we identified parameters for differentiating control biopsies from those of the patients with CeD. We repeated the quantitative histological analysis in a validation cohort (105 controls and 120 patients with CeD). On the basis of the results, we propose a quantitative histological classification system. The new classification was compared with the existing histological classifications for interobserver and intraobserver agreements by a group of qualified pathologists. Results: Among the histological parameters, intraepithelial lymphocyte count of ≥25/100 epithelial cells, adjusted villous height fold change of ≤0.7, and crypt depth-to-villous height ratio of ≥0.5 showed good discriminative power between the mucosal biopsies from the patients with CeD and those from the controls, with 90.3% sensitivity, 93.5% specificity, and 96.2% area under the curve. Among the existing histological classifications, our quantitative histological classification showed the highest intraobserver (69.7%–85.03%) and interobserver (24.6%–71.5%) agreements. Conclusions: Quantitative assessment increases the reliability of the histological assessment of mucosal biopsies in patients with CeD. Such a classification system may be used for clinical trials in patients with CeD.