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- 저자 : Patrick Keller (검색결과 2 건)
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Sarzsinszky Eszter,Lupinek Christian,Vrtala Susanne,Huang Huey-Jy,Hofer Gerhard,Keller Walter,Chen Kuan-Wei,Panaitescu Carmen Bunu,Resch-Marat Yvonne,Zieglmayer Petra,Zieglmayer René,Lemell Patrick,Ho 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.1
Arginine kinase (AK) was first identified as an allergen in the Indian-meal moth and subsequently shown to occur as allergen in various invertebrates and shellfish. The cDNA coding for AK from the house dust mite (HDM) species Dermatophagoides pteronyssinus, Der p 20, has been isolated, but no recombinant Der p 20 (rDer p 20) allergen has been produced and characterized so far. We report the expression of Der p 20 as recombinant protein in Escherichia coli. rDer p 20 was purified and shown to be a monomeric, folded protein by size exclusion chromatography and circular dichroism spectroscopy, respectively. Using AK-specific antibodies, Der p 20 was found to occur mainly in HDM bodies, but not in fecal particles. Thirty percent of clinically well-characterized HDM allergic patients (n = 98) whose immunoglobulin E (IgE) reactivity profiles had been determined with an extensive panel of purified HDM allergens (Der f 1, 2; Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 and 37) showed IgE reactivity to Der p 20. IgE reactivity to Der p 20 was more frequently associated with lung symptoms. AKs were detected in several invertebrates with specific antibodies and Der p 20 showed IgE cross-reactivity with AK from shrimp (Litopenaeus vannamei). Thus, Der p 20 is a cross-reactive HDM allergen and may serve as a diagnostic marker for HDM-induced lung symptoms such as asthma.
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Study of the optical, thermal, and mechanical properties of nematic liquid crystal elastomers
Santosh A. Mania,Sameer U. Hadkar,P. J. Jessy,Suman Lal,Patrick Keller,Samriti Khosla,Nitin Sood,Pradip Sarawade 한국정보디스플레이학회 2016 Journal of information display Vol.17 No.4
In the present study, the optical, thermal, and mechanical properties of liquid crystal elastomers (LCEs) were investigated using various techniques. The presence of functional groups in LCE was studied using Fourier transform infrared spectroscopy. The phase transition temperatures were confirmed via polarizing optical microscopy and Fabry–Perot scattering studies. The differential thermal analysis was used for investigating the thermal behavior. A dynamic mechanical analysis was used to study the mechanical properties of LCE. The significant mechanical changes with a considerable reversible effect were observed for this soft material. The changes in the mechanical shape with the temperature are attributed to the change in the phase of the LCE material.
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