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- 저자 : Palsson, Bernhanrd O. (검색결과 61 건)
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Eiteneer, D.,Palsson, G.K.,Nemsak, S.,Gray, A.X.,Kaiser, A.M.,Son, J.,LeBeau, J.,Conti, G.,Greer, A.A.,Keqi, A.,Rattanachata, A.,Saw, A.Y.,Bostwick, A.,Rotenberg, E.,Gullikson, E.M.,Ueda, S.,Kobayashi Elsevier Scientific Pub. Co 2016 Journal of electron spectroscopy and related pheno Vol.211 No.-
LaNiO<SUB>3</SUB> (LNO) is an intriguing member of the rare-earth nickelates in exhibiting a metal-insulator transition for a critical film thickness of about 4 unit cells [Son et al., Appl. Phys. Lett. 96, 062114 (2010)]; however, such thin films also show a transition to a metallic state in superlattices with SrTiO<SUB>3</SUB> (STO) [Son et al., Appl. Phys. Lett. 97, 202109 (2010)]. In order to better understand this transition, we have studied a strained LNO/STO superlattice with 10 repeats of [4 unit-cell LNO/3 unit-cell STO] grown on an (LaAlO<SUB>3</SUB>)<SUB>0.3</SUB>(Sr<SUB>2</SUB>AlTaO<SUB>6</SUB>)<SUB>0.7</SUB> substrate using soft x-ray standing-wave-excited angle-resolved photoemission (SWARPES), together with soft- and hard- x-ray photoemission measurements of core levels and densities-of-states valence spectra. The experimental results are compared with state-of-the-art density functional theory (DFT) calculations of band structures and densities of states. Using core-level rocking curves and x-ray optical modeling to assess the position of the standing wave, SWARPES measurements are carried out for various incidence angles and used to determine interface-specific changes in momentum-resolved electronic structure. We further show that the momentum-resolved behavior of the Ni 3d e<SUB>g</SUB> and t<SUB>2g</SUB> states near the Fermi level, as well as those at the bottom of the valence bands, is very similar to recently published SWARPES results for a related La<SUB>0.7</SUB>Sr<SUB>0.3</SUB>MnO<SUB>3</SUB>/SrTiO<SUB>3</SUB> superlattice that was studied using the same technique (Gray et al., Europhysics Letters 104, 17004 (2013)), which further validates this experimental approach and our conclusions. Our conclusions are also supported in several ways by comparison to DFT calculations for the parent materials and the superlattice, including layer-resolved density-of-states results.
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Halberstadt, Craig R.,Palsson, Bernhanrd O.,Midgley, A.Rees,Curl, Rane L. The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
This report describes the use of a transtubular bioreactor to study the relative effects of diffusion versus perfusion of medium on antibody production by a hybridoma cell line. The study was performed with a high-density cell culture maintained in a serum-free, low-protein medium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence time distribution similar to a continuous stirred tank reactor (CSTR), However, due to the arrangement of the medium lines in the reactor, the flow patterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cyclic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accessible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a lapped volume, which provides nutrients through unilateral convection; and a swept volume, which operates through bilateral convection. The relative sizes of these three volumes were modified experimentally by changing the period over which the direction of medium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The results suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difference in cellular metabolism occurred as the reactor was moved between these different states. These results indicate that the mode of operation of the transtubular bioreactor may influence antibody productivity under serum-free, low-protein conditions with minimal effects on cellular metabolism.
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Characterization of the KG1a Cell Line for Use in a Cell Migration Based Screening Assay
KarlFrancis,이균민,BernhardO.Palsson 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
for potentially therapeutic compounds. Further screening of these lead compounds is typicaly done with secondary assays which may utilize living, functioning cels as screening tools. A prob-lem (or benefit) with these cell-based assays is that living cells are very sensitive to their environ-ment. We have been interested in the process of stem cel migration and how it relates to the cel-lular therapy of bone marrow transplantation. In this study we describe a secondary, cel-based as-say for screening the effects of various in-vitro conditions on Imature Hematopoietic Cell (IHC) or the effect of a cultures growth phase, that need to be accounted for in a screning protocol. Fi-nally, we show that exponentially growing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly sugest that KG1a cels secrete a chemokinetic factor during the exponential growth phase of a culture.
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CraigR.Halberstadt,BernhardO.Palsson,A.ReesMidgley 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
This report describes the use of a transtubular bioreactor to study the relative efectsof diffusion versus perfusion of medium on antibody production by a hybridoma cel line. The study was performed with a high-density cel culture maintained in a serum-fre, low-protein me-dium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence rangement of the medium lines in the reactor, the flow paterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cy-clic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accesible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a laped volume, which provides nutrients through unilateral convection; volumes were modified experimentally by changing the period over which the direction of me-dium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The re-sults suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difer-ence in celular metabolism occured as the reactor was moved betwen these diferent states. tibody productivity under serum-free, low-protein conditions with minimal effects on celular me-tabolism.
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STATR: A simple analysis pipeline of Ribo-Seq in bacteria
Donghui Choe,Bernhard Palsson,Byung-Kwan Cho 한국미생물학회 2020 The journal of microbiology Vol.58 No.3
Gene expression changes in response to diverse environmental stimuli to regulate numerous cellular functions. Genes are expressed into their functional products with the help of messenger RNA (mRNA). Thus, measuring levels of mRNA in cells is important to understand cellular functions. With advances in next-generation sequencing (NGS), the abundance of cellular mRNA has been elucidated via transcriptome sequencing. However, several studies have found a discrepancy between mRNA abundance and protein levels induced by translational regulation, including different rates of ribosome entry and translational pausing. As such, the levels of mRNA are not necessarily a direct representation of the protein levels found in a cell. To determine a more precise way to measure protein expression in cells, the analysis of the levels of mRNA associated with ribosomes is being adopted. With an aid of NGS techniques, a single nucleotide resolution footprint of the ribosome was determined using a method known as Ribo- Seq or ribosome profiling. This method allows for the highthroughput measurement of translation in vivo, which was further analyzed to determine the protein synthesis rate, translational pausing, and cellular responses toward a variety of environmental changes. Here, we describe a simple analysis pipeline for Ribo-Seq in bacteria, so-called simple translatome analysis tool for Ribo-Seq (STATR). STATR can be used to carry out the primary processing of Ribo-Seq data, subsequently allowing for multiple levels of translatome study, from experimental validation to in-depth analyses. A command- by-command explanation is provided here to allow a broad spectrum of biologists to easily reproduce the analysis.
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Deciphering the regulatory codes in bacterial genomes.
Cho, Byung-Kwan,Palsson, Bernhard,Zengler, Karsten Wiley 2011 Biotechnology Journal Vol.6 No.9
<P>Interactions between cis-regulatory elements and trans-acting factors are fundamental for cellular functions such as transcription. With the revolution in microarrays and sequencing technologies, genome-wide binding locations of trans-acting factors are being determined in large numbers. The richness of the genome-scale information has revealed that the nature of the bacterial transcriptome and regulome are considerably more complex than previously expected. In addition, the emerging view of the bacterial transcriptome is revising the concept of the operon organization of the genome. This review describes current advances in the genome-scale analysis of the interaction between cis-regulatory elements and trans-acting factors in microorganisms.</P>
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LEE, GYUN MIN,SAVINELL, JOANNE,PALSSON, BERNHARD O. 한국미생물 · 생명공학회 1992 Journal of microbiology and biotechnology Vol.2 No.2
Physiological changes of the murine hybridoma cell line S3H5/γ2bA2 during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.
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