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Chemoattractant releasing microneedles for enhanced DNA vaccination
Nak Won Kim,Su Yeon Lim,Dahwun Kim,Siyan Lyu,Ouibo Whang,Chaeeun Park,Byung Deok Kim,이민상,정지훈 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-
Transcutaneous delivery of a DNA vaccine using microneedle (MN) has attracted much attention sincethere are a variety of immune cells underneath the skin. However, the clinical use of DNA vaccine is limiteddue to its poor cellular uptake of naked plasmid DNA (pDNA) and suboptimal presentation of an antigenby antigen presenting cells (APC). Herein, we designed a MN system for delivering polyplex-basedDNA vaccine and recruiting APCs by a chemoattractant N-formyl-methionyl-leucyl-phenylalanine peptide(fMLP)-releasing microspheres (fMLP-MS) using MN coated with pH-responsive polyelectrolyte multilayerassembly (PMA). The PMA can be rapidly disrupted upon the application of MN to the skin, leadingto the release of polyplex and fMLP-MS. The polyplex would be taken up by the epidermal cells and residentAPCs to express and secrete the antigen (amyloid beta monomer, Ab1-42) encoded in the DNA vaccine. fMLP released from fMLP-MS could recruit APCs to the site of MN administration by chemotacticeffects. The MN-based vaccination system was able to induce robust antigen-specific antibodyproduction.