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제한효소 Hind III , Sst I , Pvu II , Sac I 의 DNA 절단 반응에 있어서 Alu I methylation 의 억제 효과
강선철,윤호섭,유욱준 ( Sun Chul Kang,Ho sup Yoon,Oog . Joon Yoo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
The cleavage reactions of Hind III, Sst I, Pvu II and Sac I endonucleases were inhibited by the methylation of Alu I methylase. To analyze the cleavage reactions quantitatively, tritium labeled pBR 322 and pPG 3282 plasmid DNAs (pBR 322 DNA contains Hind III and Pvu II sites, while pPG 3282 DNA which is a hog gastrin cDNA clone contains Sst I and Sac I sites) were used, and the degree of the inhibition against cleavages by the four kinds of endonucleases was measured. The results imply that we can predict the specificities of the related hexameric restriction methylases which have not been isolated yet. And the predictions are the followings: (1) The methylation sites of Sst I methylase is not identical with Alu I methylase since Sst I endonuclease slowly cut the sequences methylated by Alu I methylase. (2) The methylation sites of Pvu II and Sac I methylases could be identical with ALu I methylase since Pvu II and Sac I endonucleases could not cut the sequences methylated by Alu I methylase.