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마우스 배아줄기세포를 이용한 심근세포 분화촉진 물질 탐색
남기환 ( Ki Hoan Nam ),김은경 ( Eun Kyoung Kim ),원영석 ( Young Suk Won ),문옥성 ( Ok Seong Moon ),안병우 ( Byeong Woo Ahn ),김형진 ( Hyoung Chin Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
Embryonic stem cells are derived from inner cell mass of blastcyst. They are pluripotent in differentiation and show self-renewal ability. Therefore, the cells can differentiate into cell types which can be derived from all the three germ layers. In this report, we screened 100 compounds for their potential effects on the differentiation of KH2 mouse embryonic stem cells to cardiomyocytes. KH2 cells are developed in our lab and express enhanced green fluorescence protein under the direction of α-myosin heavy chain gene promoter. This cell line shows no fluorescence in undifferentiated condition. However, cardiomyocytes derived from the cells show green fluorescence. Test compound was added during the differentiation period of the cells. After 15 days of differentiation, the cells were trypsinized and measured by flow cytometry for their fluorescence protein expression. All tested compound did not show any significant increase in the rate of fluorescent cells compared with that of their control group. However, we could confirm that our cell screening system is very stable.
연구논문 : 마우스 배아줄기세포를 이용한 심근세포 분화촉진 물질 탐색
남기환 ( Ki Hoan Nam ),김은경 ( Eun Kyoung Kim ),원영석 ( Young Suk Won ),문옥성 ( Ok Seong Moon ),안병우 ( Byeong Woo Ahn ),김형진 ( Hyoung Chin Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
Embryonic stem cells are derived from inner cell mass of blastcyst. They are pluripotent in differentiation and show self-renewal ability. Therefore, the cells can differentiate into cell types which can be derived from all the three germ layers. In this report, we screened 100 compounds for their potential effects on the differentiation of KH2 mouse embryonic stem cells to cardiomyocytes. KH2 cells are developed in our lab and express enhanced green fluorescence protein under the direction of α-myosin heavy chain gene promoter. This cell line shows no fluorescence in undifferentiated condition. However, cardiomyocytes derived from the cells show green fluorescence. Test compound was added during the differentiation period of the cells. After 15 days of differentiation, the cells were trypsinized and measured by flow cytometry for their fluorescence protein expression. All tested compound did not show any significant increase in the rate of fluorescent cells compared with that of their control group. However, we could confirm that our cell screening system is very stable.
마우스배아줄기세포의 in vitro 시험계 활용을 위한 신경세포 분화프로토콜의 비교
김해림 ( Hae Rim Kim ),남기환 ( Ki Hoan Nam ),김은경 ( Eun Kyoung Kim ),윤원기 ( Won Ki Yoon ),원영석 ( Young Suk Won ),문옥성 ( Ok Seong Moon ),정의배 ( Eu Bae Joung ),안병우 ( Byeong Woo Ahn ),김형진 ( Hyoung Chin Kim ) 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1
Mouse embryonic stem cells are pluripotent stem cells that can be differentiate into all the cell types derived from three germ layers in vitro. We aimed to confirm the neuronal cell types derived from the embryonic stem cells by two different differentiation protocols, which would guide us which protocol is useful for in vitro neuronal toxicity test. The mouse embryonic stem cells derived from 129 mouse strain, TC-1 cells, were differentiated according to 30-day differentiation protocol or 15-day differentiation protocol. At the end of the differentiation period, neuronal cells (neuron, astrocyte and oligodendrocyte) were identified by immunocytochemistry using marker antibodies. According to the results, the numbers of astrocytes and oligodendrocytes were much higher than that of neurons in 30-day differntiation protocol. However, oligodendrocytes were overwhelming compared to astrocytes and neurons in the 15-day differntiation protocol. These results indicated that the neuronal cell types and the cell numbers derived from the embryonic stem cells should be considered when selecting in vitro neuronal cell toxicity models using embryonic stem.