Optimal panel of immunohistochemistry for the diagnosis of B-cell non-Hodgkin lymphoma using bone marrow biopsy: a tertiary care center study

Nisha Marwah,Manali Satiza,Niti Dalal,Sudhir Atri,Monika Gupta,Sunita Singh,Rajeev Sen 대한혈액학회 2021 Blood Research Vol.56 No.1

Background Morphological diagnosis of non-Hodgkin lymphoma (NHL) is usually based on lymph node biopsy. Bone marrow biopsy (BMB) is important for staging, and morphology alone can be challenging for subtyping. Immunohistochemistry (IHC) allows a more precise diagnosis and characterization of NHL using monoclonal antibodies. However, there is a need for a minimal panel that can provide maximum information at an affordable cost. Methods All newly diagnosed cases of B-cell NHL with bone marrow infiltration between 2017 and 2019 were included. BMB was the primary procedure for diagnosing B-cell NHL. Subtyping of lymphomas was performed by immunophenotyping using a panel of monoclonal antibodies on IHC. The primary diagnostic panel of antibodies for B-cell NHL included CD19, CD20, CD79, CD5, CD23, CD10, Kappa, and Lambda. The extended panel of antibodies for further subtyping included CD30, CD45, CD56, Cyclin D1, BCL2, and BCL6. Results All cases of B-cell NHL were classified into the chronic lymphocytic leukemia (CLL) and non-CLL groups based on morphology and primary IHC panel. In the CLL group, the most significant findings were CD5 expression, CD23 expression, dim CD79 expression, and weak surface immunoglobulin (Ig) positivity. In the non-CLL group, they were CD5 expression, positive or negative CD23 expression, strong CD79 expression, and strong surface Ig expression. An extended panel was used for further subtyping of non-CLL cases, which comprised CD10, Cyclin D1, BCL2, and BCL6. Conclusion We propose a two-tier approach for immunophenotypic analysis of newly diagnosed B-cell NHL cases with a minimum primary panel including CD5, CD23, CD79, Kappa, and Lambda for differentiation into CLL/non-CLL group and Kappa and Lambda for clonality assessment. An extended panel may be used wherever required for further subtyping of non-CLL.

Morphological and cytogenetic analysis of different cytotypes of Boerhaavia diffusa L. and their evaluation for biological activity

Sharma Nisha,Singh Bikram,Bhatia Aruna,Gupta Raghbir Chand,Wani Mohammad Saleem 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.4

Boerhavia diffusa is an herbaceous plant of the family Nyctaginaceae. It is mentioned to have anti-aging, disease prevention, and life-enhancing activities that hold enormous influence on disease burden and affordability/availability of healthcare in the world. In the present study, various populations of this plant species were characterized using morphological markers, cytogenetic and phytochemical analyses. Besides, biological activity was also studied for anti-diabetic and anti-bacterial effects using rats and bacterial systems respectively. For the three cytotypes i.e. diploid, tetraploid and octaploid, twelve morphological traits were studied. HPLC system was used for the identification of bioactive compounds present in extracts. The in vivo anti-hyperglycemic effects of the extract in alloxan- induced diabetes were determined using Swiss albino rats. Rats with fasting plasma glucose vary ≥ 300–315 mg/dl were treated to be diabetic and utilized for the examination. Anti-bacterial activities of three cytotypes of B. diffusa were screened by using the Disc diffusion method against two strains of Gram-positive bacteria and three strains of Gram-negative bacteria. The present study reports the existence of diploid, tetraploid and octaploid cytotypes which was new report from India. The diploid cytotype of B. diffusa showed maximum activity against diabetes in alloxan-induced diabetic rats and more anti-bacterial activity in Gram-positive and Gram-negative bacteria as compared to tetraploid and octaploid cytotypes. HPLC results showed that the concentration of phytoconstituents showed a negative correlation with the ploidy level being maximum in plants with lower ploidy levels. The present paper represents the first report of the pharmacognostic study based on different plant parts and cytotypes in B. diffusa. Results of the present study will be useful for exploiting appropriate cytotypes for various purposes such as formulating new anti-diabetic and anti-bacterial drugs from B. diffusa.

Assessment of Bioremediation Potential of Cellulosimicrobium sp. for Treatment of Multiple Heavy Metals

Bhati, Tushar,Gupta, Rahul,Yadav, Nisha,Singh, Ruhi,Fuloria, Antra,Waziri, Aafrin,Chatterjee, Sayan,Purty, Ram Singh The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2

In the present study, we have studied the bioremediating capability of bacterial strain against six heavy metals. The strain was isolated from river Yamuna, New Delhi which is a very rich repository of bioremediating flora and fauna. The strain was found to be Gram positive as indicated by Gram staining. The strain was characterized using 16s rRNA gene sequencing and the BlastN result showed its close resemblance with the Cellulosimicrobium sp. As each treatment has its own toxicity eliciting expression of different factors, we observed varied growth characteristics of the bacterial isolate and its protein content in response to different heavy metals. The assessment of its bioremediation capability showed that the strain Cellulosimicrobium sp. has potential to consume or sequester the six heavy metals in this study in the following order iron > lead > zinc > cooper > nickel > cadmium. Thus, the strain Cellulosimicrobium sp. isolated in the present study can be a good model system to understand the molecular mechanism behind its bioremediating capabilities under multiple stress conditions.

Assessment of Bioremediation Potential of Cellulosimicrobium sp. for Treatment of Multiple Heavy Metals

Tushar Bhati,Rahul Gupta,Nisha Yadav,Ruhi Singh,Antra Fuloria,Aafrin Waziri,Sayan Chatterjee,Ram Singh Purty 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2

In the present study, we have studied the bioremediating capability of bacterial strain against six heavy metals. The strain was isolated from river Yamuna, New Delhi which is a very rich repository of bioremediating flora and fauna. The strain was found to be Gram positive as indicated by Gram staining. The strain was characterized using 16s rRNA gene sequencing and the BlastN result showed its close resemblance with the Cellulosimicrobium sp. As each treatment has its own toxicity eliciting expression of different factors, we observed varied growth characteristics of the bacterial isolate and its protein content in response to different heavy metals. The assessment of its bioremediation capability showed that the strain Cellulosimicrobium sp. has potential to consume or sequester the six heavy metals in this study in the following order iron > lead > zinc > cooper > nickel > cadmium. Thus, the strain Cellulosimicrobium sp. isolated in the present study can be a good model system to understand the molecular mechanism behind its bioremediating capabilities under multiple stress conditions.

Effect of Neem (Azadirachta indica) oil on the progressive growth of a spontaneous T cell lymphoma

Mallick, Sanjaya Kumar,Gupta, Vivekanand,Singh, Mahendra Pal,Vishvakarma, Naveen Kumar,Singh, Nisha,Singh, Sukh Mahendra Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.7 No.5

The present study was undertaken to investigate the effect of in vivo administration of neem oil intra-peritoneally (i.p.) to mice bearing a progressively growing transplantable T cell lymphoma of spontaneous origin, designated as Daltons lymphoma (DL), on the tumor growth. Mice were administered various doses of neem oil mixed in groundnut oil, which was used as a diluting vehicle or for administration to control DL-bearing mice. Administration of neem oil resulted in an acceleration of tumor growth along with a reduction in the survival time of the tumor-bearing host. Neem oil administered DL-bearing mice showed an augmented apoptosis in splenocytes, bone marrow cells and thymocytes along with an inhibition in the anti-tumor functions of tumor-associated macrophages. Thus this study gives an altogether a novel information that neem oil instead of the popular belief of being anti-tumor and immunoaugmentary may in some tumor-bearing conditions, behave in an opposite way leading to an accelarated tumor progression along with a collapse of the host's anti-tumor machinery. These observations will thus have long lasting clinical significance, suggesting caution in use of neem oil for treatment of cancer.

Design of Wideband Inverted Sinc Shaped Monopole Antenna for Wireless Applications

Ritesh Kumar Badhai,Nisha Gupta 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.1

A novel wideband inverted Sinc shaped printed monopole antenna is proposed for wireless devices. The proposed configuration not only realizes the monopole antenna in compact form but also depicts good radiation characteristics for wideband operations. A detailed parametric study has been carried out for different size of the ground plane, feed gap and Sinc strip length. By selecting the proper dimensions of ground plane, feed gap and Sinc strip length, the broadband operation for the proposed design can be achieved and the obtained impedance bandwidth covers the 2855-5081 MHz band. The proposed monopole antenna provides a wide frequency band of about 56% for wireless operation. The peak gain and efficiency of the proposed antenna are 4.05 dBi and 92.20 % respectively. Finally a prototype model is developed for the most effective configuration and the simulated results are validated experimentally. It is seen that the proposed antenna occupies small area and is suitable for operation at radio navigation, space research, amateur radio, aviation, public safety mobile, fixed mobile and fixed satellite communication frequencies.