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Nguyen, Thi Kim Lien,Nguyen, Thanh Luan,Cao, Xuan Thang,Park, Chan,Lim, Kwon Taek Informa UK (TaylorFrancis) 2016 Molecular Crystals and Liquid Crystals Vol.635 No.1
<P>The composite material based on poly(oligo(ethylene glycol) methacrylate)-grafted-layered double hydroxides/Gentamicin (LDHs-g-POEGMA-GE) was prepared by the reversible addition fragmentation chain transfer polymerization, and its antibacterial property was investigated. Initially, the surface of LDHs was modified by S-(3-trimethoxysilyl) propyl trithiocarbonate as a chain transfer agent and then grafted with POEGMA. Subsequently, GE was covalently linked to the POEGMA chains by esterification reaction producing LDHs-g-POEGMA-GE. The composites were characterized by using FT-IR, GPC, XRD, TGA, and SEM analyses. The in-vitro assay with Staphylococcus aureus bacteria indicated the high antibacterial activity of the composites, which promises potential applications in the biomaterial field.</P>
Nguyen, Phi-Hung,Yang, Jun-Li,Uddin, Mohammad N.,Park, So-Lim,Lim, Seong-Il,Jung, Da-Woon,Williams, Darren R.,Oh, Won-Keun American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.11
<P>As part of our ongoing search for new antidiabetic agents from medicinal plants, we found that a methanol extract of <I>Morinda citrifolia</I> showed potential stimulatory effects on glucose uptake in 3T3-L1 adipocyte cells. Bioassay-guided fractionation of this active extract yielded two new lignans (<B>1</B> and <B>2</B>) and three new neolignans (<B>9</B>, <B>10</B>, and <B>14</B>), as well as 10 known compounds (<B>3</B>–<B>8</B>, <B>11</B>–<B>13</B>, and <B>15</B>). The absolute configurations of compounds <B>9</B>, <B>10</B>, and <B>14</B> were determined by ECD spectra analysis. Compounds <B>3</B>, <B>6</B>, <B>7</B>, and <B>15</B> showed inhibitory effects on PTP1B enzyme with IC<SUB>50</SUB> values of 21.86 ± 0.48, 15.01 ± 0.20, 16.82 ± 0.42, and 4.12 ± 0.09 μM, respectively. Furthermore, compounds <B>3</B>, <B>6</B>, <B>7</B>, and <B>15</B> showed strong stimulatory effects on 2-NBDG uptake in 3T3-L1 adipocyte cells. This study indicated the potential of compounds <B>3</B>, <B>6</B>, <B>7</B>, and <B>15</B> as lead molecules for antidiabetic agents.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-11/np400533h/production/images/medium/np-2013-00533h_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400533h'>ACS Electronic Supporting Info</A></P>
Lim, Dong Kyu,Mo, Changyeun,Long, Nguyen Phuoc,Lim, Jongguk,Kwon, Sung Won Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1061 No.-
<P><B>Abstract</B></P> <P>The expansion of the global rice marketplace ultimately raises concerns about authenticity control. Several analytical methods for differentiating the geographical origin of rice have been developed, yet a high-throughput method is still in demand. In this study, we developed a rapid approach using direct infusion-mass spectrometry (DI-MS) to distinguish rice products from different countries. Specifically, the elimination of the matrix effect by a polytetrafluoroethylene (PTFE) filter, a mixed-mode cation exchange (MCX) solid-phase extraction (SPE) with 20% methanol, and an MCX SPE with 100% methanol were measured. Afterward, partial least squares discriminant analysis and random forests were applied to seek the optimal discrimination method. The results revealed that the combination of MCX SPE with 100% methanol and DI-MS in positive ion mode (accuracy=1.000, R<SUP>2</SUP> =0.916, Q<SUP>2</SUP> =0.720, B/W-based <I>p</I>-value=0.015) or the combination of MCX SPE with 20% methanol and targeted DI-MS/MS in positive ion mode (accuracy=1.000, R<SUP>2</SUP> =0.931, Q<SUP>2</SUP> =0.849, B/W-based <I>p</I>-value=0.002) showed the excellent discriminatory ability. Furthermore, differentially expressed metabolites including sodiated lysophosphatidylcholine, lysophosphatidylcholine, lysophosphatidylethanolamines and lysophosphatidylglycerol classes were found. In conclusion, our study provides a rapid and reliable platform for geographical discrimination of white rice and will contribute to the authenticity control of rice products.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lim, Dong Kyu,Long, Nguyen Phuoc,Mo, Changyeun,Dong, Ziyuan,Lim, Jongguk,Kwon, Sung Won Oxford University Press 2018 Journal of AOAC International Vol.101 No.2
<B>Abstract</B><P>In this study, we examined the effects of different extraction methods for the GC-MS- and LC-MS-based metabolite profiling of white rice (Oryza sativa L.). In addition, the metabolite divergence of white rice cultivated in either Korea or China was also evaluated. The discrimination analysis of each extraction method for white rice from Korea and China and the corresponding discriminatory markers were estimated by unpaired t-test, principal component analysis, k-means cluster analysis, partial least-squares discriminant analysis (PLS-DA), and random forest (RF). According to the prediction parameters obtained from PLS-DA and RF classifiers as well as features that could be identified, the extraction method using 75% isopropanol heated at 100°C coupled with LC-MS analysis was confirmed to be superior to the other extraction methods. Noticeably, lysophospholipid concentrations were significantly different in white rice between Korea and China, and they are novel markers for geographical discrimination. In conclusion, our study suggests an optimized extraction and analysis method as well as novel markers for the geographical discrimination of white rice.</P>
Flagellin-dependent TLR5/caveolin-1 as a promising immune activator in immunosenescence
Lim, Jae Sung,Nguyen, Kim Cuc Thi,Nguyen, Chung Truong,Jang, Ik-Soon,Han, Jung Min,Fabian, Claire,Lee, Shee Eun,Rhee, Joon Haeng,Cho, Kyung A BLACKWELL PUBLISHING 2015 AGING CELL Vol.14 No.5
<P>The age-associated decline of immune responses causes high susceptibility to infections and reduced vaccine efficacy in the elderly. However, the mechanisms underlying age-related deficits are unclear. Here, we found that the expression and signaling of flagellin (FlaB)-dependent Toll-like receptor 5 (TLR5), unlike the other TLRs, were well maintained in old macrophages, similar to young macrophages. The expression and activation of TLR5/MyD88, but not TLR4, were sensitively regulated by the upregulation of caveolin-1 in old macrophages through direct interaction. This interaction was also confirmed using macrophages from caveolin-1 or MyD88 knockout mice. Because TLR5 and caveolin-1 were well expressed in major old tissues including lung, skin, intestine, and spleen, we analyzed in vivo immune responses via a vaccine platform with FlaB as a mucosal adjuvant for the pneumococcal surface protein A (PspA) against <I>Streptococcus pneumoniae</I> infection in young and aged mice. The FlaB-PspA fusion protein induced a significantly higher level of PspA-specific IgG and IgA responses and demonstrated a high protective efficacy against a lethal challenge with live <I>S. pneumoniae</I> in aged mice. These results suggest that caveolin-1/TLR5 signaling plays a key role in age-associated innate immune responses and that FlaB-PspA stimulation of TLR5 may be a new strategy for a mucosal vaccine adjuvant against pneumococcal infection in the elderly.</P>
Lim, Dong Kyu,Mo, Changyeun,Lee, Dong-Kyu,Long, Nguyen Phuoc,Lim, Jongguk,Kwon, Sung Won The Journal of Food and Drug Analysis (JFDA), Food 2018 JOURNAL OF FOOD AND DRUG ANALYSIS Vol.26 No.1
<P>The authenticity determination of white rice is crucial to prevent deceptive origin labeling and dishonest trading. However, a non-destructive and comprehensive method for rapidly discriminating the geographical origins of white rice between countries is still lacking. In the current study, we developed a volatile organic compound based geographical discrimination method using headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) to discriminate rice samples from Korea and China. A partial least squares discriminant analysis (PLS-DA) model exhibited a good classification of white rice between Korea and China (accuracy = 0.958, goodness of fit = 0.937, goodness of prediction = 0.831, and permutation test p-value = 0.043). Combining the PLS-DA based feature selection with the differentially expressed features from the unpaired t-test and significance analysis of microarrays, 12 discriminatory biomarkers were found. Among them, hexanal and 1-hexanol have been previously known to be associated with the cultivation environment and storage conditions. Other hydrocarbon biomarkers are novel, and their impact on rice production and storage remains to be elucidated. In conclusion, our findings highlight the ability to rapidly discriminate white rice from Korea and China. The developed method maybe useful for the authenticity and quality control of white rice. Copyright (C) 2017, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.</P>