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      • KCI등재

        Rhizospheric-Derived Nocardiopsis alba BH35 as an Effective Biocontrol Agent Actinobacterium with Antifungal and Plant Growth-Promoting Effects: In Vitro Studies

        El-Sayed Mohamed H.,Kobisi  Abd El-Nasser A.,Elsehemy  Islam A.,El-Sakhawy and  Mohamed A. 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.5

        The biocontrol approach using beneficial microorganisms to control crop diseases is becoming an essential alternative to chemical fungicides. Therefore, new and efficient biocontrol agents (BCA) are needed. In this study, a rhizospheric actinomycete isolate showed unique and promising antagonistic activity against three of the most common phytopathogenic fungi, Fusarium oxysporum MH105, Rhizoctonia solani To18, and Alternaria brassicicola CBS107. Identification of the antagonistic strain, which was performed according to spore morphology and cell wall chemotype, suggested that it belongs to the Nocardiopsaceae. Furthermore, cultural, physiological, and biochemical characteristics, together with phylogenetic analysis of the 16S rRNA gene (OP869859.1), indicated the identity of this strain to Nocardiopsis alba. The cell-free filtrate (CFF) of the strain was evaluated for its antifungal potency, and the resultant inhibition zone diameters ranged from 17.0 ± 0.92 to 19.5 ± 0.28 mm for the tested fungal species. Additionally, the CFF was evaluated in vitro to control Fusarium wilt disease in Vicia faba using the spraying method under greenhouse conditions, and the results showed marked differences in virulence between the control and treatment plants, indicating the biocontrol efficacy of this actinomycete. A promising plant-growth promoting (PGP) ability in seed germination and seedling growth of V. faba was also recorded in vitro for the CFF, which displayed PGP traits of phosphate solubilization (48 mg/100 ml) as well as production of indole acetic acid (34 μg/ml) and ammonia (20 μg/ml). This study provided scientific validation that the new rhizobacterium Nocardiopsis alba strain BH35 could be further utilized in bioformulation and possesses biocontrol and plant growth-promoting capabilities.

      • Catalytic hydrolysis of ammonia borane for hydrogen generation using Cu(0) nanoparticles supported on TiO<sub>2</sub> nanofibers

        Yousef, Ayman,Barakat, Nasser A.M.,EL-Newehy, Mohamed H.,Ahmed, M.M.,Kim, Hak Yong Elsevier 2015 Colloids and surfaces. A, Physicochemical and engi Vol.470 No.-

        <P><B>Abstract</B></P> <P>Cu(0) nanoparticles supported on TiO<SUB>2</SUB> nanofibers (Cu-decorated TiO<SUB>2</SUB> NFs) are introduced as an effective photocatalyst for hydrolytic dehydrogenation of ammonia borane (AB) under solar radiation. The introduced Cu-decorated TiO<SUB>2</SUB> NFs were prepared using electrospinning followed by hydrothermal treatment as a novel route for preparation of metal-decorated metal oxide nanofibers. Interestingly, the utilized physicochemical characterizations showed that the prepared nanocatalyst composed of core TiO<SUB>2</SUB> NFs decorated by Cu(0) NPs. A strong photocatalytic activity for catalytic hydrolysis of AB under solar radiation using the introduced nanocatalysts was observed. The results reveal that high rate of hydrogen release from ammonia borane (2.75 equivalent moles) as compared to undecorated TiO<SUB>2</SUB> NFs (1.6 equivalent moles) and pristine Cu NPs (1.2 equivalent moles). The obtained high hydrogen production, in case of the introduced Cu-decorated TiO<SUB>2</SUB> NFs, is attributed to the dual effect of counter parts. Furthermore, the introduced nanofibers could be utilized for three successive cycles with the same efficiency which reflects high stability. Moreover, the photocatalytic activity of the introduced NFs was further confirmed by photodegradation of methylene blue (MB) under solar radiation; complete removal was observed after 120min.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cu(0) nanoparticles covered surface of titanium dioxide nanofibers. </LI> <LI> High catalytic activity in hydrogen release from ammonia borane. </LI> <LI> Prepared photocatalyst can be reused with the same activity. </LI> </UL> </P>

      • KCI등재

        Substantial Functional Finishing and Transfer Printing of Polyester Fabric Using Zinc Oxide/Polyurethane Nanocomposite

        Samiha M. Abo El-Ola,Magda A. El-Bendary,Nasser H. Mohamed,Rehab M. Kotb 한국섬유공학회 2022 Fibers and polymers Vol.23 No.10

        Functionalization of the textile surface plays a remarkable role in the synthetic fiber industry. This research isinterested in the one-step simultaneous functional finishing and transfer printing of alkaline-activated polyester fabric usingC.I. Disperse Red 60 and C.I. Disperse Blue 56. The optimum color depth was achieved using zinc oxide nanoparticle 0.5 %and nano polyurethane (50 g/l) in the finishing bath formulation, additionally, excellent ultraviolet protection (50+) category. Zinc oxide nanoparticles and zinc oxide/polyurethane nanocomposite finishing showed high antimicrobial efficiency. Theyshowed a microbial reduction of C. albicans (97 %), E. coli (93 and 96 %), and S. aureus (88 and 93 %), respectively. Thefinished polyester fabrics showed significant durability of protective properties for washing cycles after 25 washes. Theultraviolet protection maintained excellent category and enhanced ultraviolet protection factor values. The antimicrobialactivities after 25 washes achieved sustained microbial reduction percentage against the three tested microorganisms (C. albicans 92 and 87 %; E. coli 90 and 92 %; and S. aureus 87 and 81 %). The results showed the positive impact of zinc oxidenanoparticles and zinc oxide/polyurethane nanocomposite finishing on fastness to sublimation and light. At the same time,there is no change in the fastness of washing and perspiration. The effect of finishing formulations on the finished polyesterfabric samples' crucial mechanical properties was studied. These properties were fabric weight, thickness, tensile strength andelongation, and abrasion resistance. The obtained results showed that finishing polyester fabric samples with nanopolyurethane, zinc oxide nanoparticles, or zinc oxide/polyurethane nanocomposite did not alter the essential mechanicalproperties required for polyester fabric applications in textiles and apparel for different end uses. Additionally, the polyesterfabric surface was characterized using scanning electron microscopy and Fourier transform infrared spectroscopy, improvingthe functional finishing.

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