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- 저자 : Narasaki, Yukie (검색결과 3 건)
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Motokawa, Shogo,Narasaki, Yukie,Song, Jun-Young,Yokoyama, Yoshihiro,Hirose, Euichi,Murakami, Shoko,Jung, Sung-Ju,Oh, Myung-Joo,Nakayama, Kei,Kitamura, Shin-Ichi Elsevier 2018 Parasitology international Vol.67 No.2
<P><B>Abstract</B></P> <P>The ciliate <I>Miamiensis avidus</I> causes scuticociliatosis in Japanese flounder <I>Paralichthys olivaceus</I>. We previously reported three serotypes of this ciliate distinguishable by serotype-specific antigenic polypeptides (serotype I, 30kDa; serotype II, 38kDa; serotype III, 34kDa). In this study, we determined the localization site of the serotype-specific polypeptides in the ciliate and determined the genes encoding the polypeptides, using the isolates IyoI (serotype I), Nakajima (serotype II), and Mie0301 (serotype III). SDS-PAGE and immunoblot analysis of cilia, membrane proteins, and cytoskeletal elements of the ciliates revealed that the polypeptides were abundant in the former two. Scanning electron microscopy of ciliates immobilized by homologous antiserum showed morphological changes in the cilia. These evidences suggested that the polypeptides were ciliary membrane immobilization antigens. The ciliary genes identified showed low identity scores—<51.5% between serotypes. To differentiate the serotypes, we designed serotype-specific PCR primer sets based on the DNA sequences. The PCR-based serotyping results were completely consistent with conventional serotyping methods (immobilization assay and immunoblot analysis). Twenty of 21 isolates were classified as either serotype I or II, and one isolate was undistinguishable. The combination of species-specific PCR previously reported and three serotype-specific PCR could be useful for identifying, serotyping, and surveillance for occurrences of new serotypes of <I>M. avidus</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three serotypes of <I>M. avidus</I> were reported in Japan and Korea. </LI> <LI> Serotype-specific polypeptides were identified as ciliary membrane proteins. </LI> <LI> The nucleotide sequences of ORFs were determined. </LI> <LI> Serotype-specific PCR revealed that the pandemic serotypes were serotype I and II. </LI> </UL> </P>
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Asian, Aniwaru,Okamoto, M.,Yoshihira, T.,Ataku, K.,Narasaki, N. Asian Australasian Association of Animal Productio 1997 Animal Bioscience Vol.10 No.6
The changes of tissue structure in timothy and alfalfa during ensiling process with silage additives; lactic acid bacteria, cellulase and formic acid, were observed with a video microscope. Stem samples were obtained from the second internode, and cut to divide into 2 pieces. One piece was for observation of ensiled material and the other was for silage. The latter piece was put into a nylon cloth bag, and ensiled with grass for 50 days in a small experimental silo Lignification of the plant tissues was checked by acid phloroglucinol. Natural silage fermentation resulted in some degradation of less lignified parenchyma in both plant species. However, lignified sclerenchyma and vascular bundles remained intact. The cellulase enhanced the degradation of parenchyma tissue, while the formic acid suppressed the degradation. The effect of lactobacillus was small. The percentage of remained cross sectional area of stem and the loss of NDF and ADF by silage fermentation confirmed the observation. High negative correlations were obtained between the remained area and loss of fibrous components during silage fermentation in both plants, and between the loss of fibrous components and in vitro dry matter digestibility in timothy but not in alfalfa.
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