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      • SCIESCOPUSKCI등재
      • SCIESCOPUSKCI등재

        관수량 조절이 시설토마토의 생육과 품질에 미치는 영향

        강남준(Nam Jun Kang),최영하(Young Hah Choi) 한국원예학회 2009 원예과학기술지 Vol.27 No.1

        Influence of irrigation levels by different soil moisture-based water potentials on plant growth and fruit quality in fresh tomato were investigated. Amount of irrigation was saved about 11.5, 30.4 and 49.3% at -20, -25 and -30 ㎪ treatment compared to the -15 ㎪ treatment, respectively. The total dry weight in ‘Superdotaerang’ cultivar was significantly reduced by 4.8, 21.2 and 34.0% in -20, -25 and -30 ㎪ treated plants compared to the -15 ㎪ treated plants, respectively. However, differential patterns of total dry weight did not occur in ‘Rapito’ cultivar by reduction of irrigation amount. Although fruit weight in ‘Superdotaerang’ cultivar was reduced by 20.9% at -30 ㎪ treatment compared to the -15 ㎪ treatment, total soluble solids was significantly increased by 27.8% at 7.13 °Brix compared with 5.58 °Brix in the fruit of -15 ㎪ treated plants. However, fruit weight and total soluble solids in ‘Rapito’ cultivar did not differ by reduction of irrigation amount. In Superdotaerang cultivar, content of glucose and fructose in fruit of -30 ㎪ treated plants was higher at 70.6% and 53.5% than those in fruit of -15 ㎪ treated plants, respectively. The contents of glucose and fructose in the fruit of ‘Rapito’ cultivar increased by 17.6% and 14.4% in the fruit of -30 ㎪ treated plants compared with 14.47 ㎎ and 13.45 ㎎ per g fresh weight of fruit of -15 ㎪ treated plants, respectively. The content of citric acid and malic acid in ‘Superdotaerang’ cultivar were increased to 62.4% and 34.5% by -30 ㎪ treatment compared with 3.22 ㎎ and 0.776 ㎎ per g fresh weight of fruit of -15 ㎪ treated plants, respectively. However, content of citric acid and malic acid by reduction of irrigation amount did not differ in ‘Rapito’ cultivar.

      • SCIESCOPUSKCI등재

        Accumulation of Soluble Solids and Activation of Antioxidant Enzymes by Deficit Irrigation in Fresh Tomato Fruits

        Nam Jun Kang(강남준),Myeong Whan Cho(조명환),Kyung Hee Kang(강경희) 한국원예학회 2009 원예과학기술지 Vol.27 No.3

        The effects of deficit irrigation on the accumulation of soluble solids and activation of antioxidant enzymes in fresh tomato (Lycopersicon esculentum Mill) fruits were investigated. Although fruit weight was reduced by deficit irrigation, dry matter was significantly increased. Deficit irrigation significantly increased sugars and organic acids compared with the normal irrigation. Content of hexoses (glucose and fructose) in fruits of moderate deficit-irrigated (MDI, -30 ㎪) and severe deficit-irrigated (SDI, -40 ㎪) plants was higher as 127.6% and 157.8% than those in fruit of normal-irrigated (NI, -20 ㎪) plants. Content of citric acid was significantly increased to 127.0% and 160.7% by MDI and SDI compared with the fruit of NI plants, respectively. Activation of antioxidant enzymes exhibited differential patterns in terms of specific and gel activity by irrigation levels through developmental stages. The specific and gel activities of SOD, POD, PPO and G6PDH in fruits of NI plants were activated 50 d at ripening stage after anthesis. However, both activities of these antioxidant enzymes are activated as early as 10 d and 20 d by MDI and SDI treatment, respectively. These results suggest that accumulation of soluble solids by deficit irrigation is associated with activation of antioxidant enzymes, and its activation seems to play a critical role in the restriction of fruit size and acceleration of ripening.

      • KCI등재

        토마토 유묘에 있어서 저온과 수분 스트레스에 대한 황산화효소의 활성 차이

        강남준(Nam Jun Kang),조명환(Myeong Whan Cho),이한철(Han Chul Rhee),최영하(Young Hah Choi),엄영철(Yeong Cheol Um) (사)한국생물환경조절학회 2007 생물환경조절학회지 Vol.16 No.2

        저온 또는 건조 처리에 따른 토마토 유묘의 생육과 부위별 항산화효소의 반응 양상을 분석한 결과, 토마토 유묘의 생체중은 처리 후 12일째에 대조구에 비해 각각 69.5%와 50.6% 감소하였다. SOD과 POD의 활성은 대조구에 비해 저온 또는 건조 처리에서 높은 활성을 보였는데, 저온 처리시에는 뿌리에서 더 높은 활성을 보였고 건조 처리에서는 잎과 줄기에서 높은 활성을 보였다. 이러한 결과는 동위효소의 발현양상에서도 일치하였다. OR의 활성은 저온 또는 건조 처리시 대조구보다 높은 활성을 보였는데, 잎과 줄기에서는 저온과 건조 처리간의 차이는 없었지만, 뿌리에서는 건조처리가 높은 경향을 보였다. OR 동위효소 발현양상은 저온과 건조처리시에는 OR-3 밴드가 잎에서는 발현되어 대조구와 차이가 있었지만, 줄기와 뿌리에서는 큰 차이가 없었다. PPO 활성은 잎에서는 모든 처리에서 차이가 없었지만, 줄기와 뿌리에서는 저온 또는 건조처리에서 대조구보다 높은 경향을 보였다. 특히 줄기의 PPO 활성은 저온 처리보다 건조처리에서 높았고 뿌리의 PPO 활성은 건조 처리보다 저온 처리에서 높았다. 동위효소의 발현양상에서도 건조처리에서는 줄기에서, 저온 처리에서는 뿌리에서 높은 밀도를 보여 불량 환경에 따른 부위별 반응 차이를 잘 반영해 주었다. Responses of antioxidant enzymes on chilling and drought stress in tomato seedlings were investigated. Growing patterns of tomato based on fresh weight of tomato seedlings were severely affected by chilling and drought stress. Fresh weight of tomato seedlings were reduced by 69.5% in chilling stress and 50.6% in drought stress compared to those in the unstressed control seedlings after 12 days of stress. The specific and gel activity of SOD and POD in the leaves, shoots, and roots of tomato seedlings were significantly increased by chilling and drought stress. Activation of SOD and POD activity by chilling stress were higher in the roots than those of drought stress. However, activation of SOD and POD activity by drought stress were higher in the leaves and shoots than those of chilling stress. The specific and gel activity of GR in the leaves, shoots, and roots of tomato seedlings were also significantly increased by chilling and drought stress. When the seedlings were treated with chilling or drought stress, one GR isozyme band (GR-3) was newly expressed in the leaves of tomato seedlings. The specific and gel activity of PPO was significantly increased in the roots and shoots of tomato seedlings by chilling and drought stress, respectively. However, the specific and gel activity of PPO in the leaves is no difference between stressed and controlled tomato seedlings.

      • KCI등재

        Changes in the Activities of Antioxidant Enzymes during Chilling Stress in Chilling-Tolerant and Chilling-Sensitive Cultivars of Cucurbita spp

        Nam Jun Kang(강남준),Joon Kook Kwon(권준국),Yong Seop Cho(조용섭),Young Hah Choi(최영하) (사)한국생물환경조절학회 2007 시설원예‧식물공장 Vol.16 No.1

        저온에 대한 생육 반응이 다른 호박 두 품종 간 항산화효소의 활성 변화를 분석해 본 결과, 저온에 강한 ‘흑종’의 잎에는 Rm이 0.20인 Mn-SOD와 와 0.52인 Fe-SOD가 주된 밴드였고, 저온에 약한 ‘재래 13호’의 잎에는 Rm이 0.20인 Mn-SOD와 0.58인 Cu/Zn-SOD가 주된 밴드였다 저온 처리 후 10일째에 ‘흑종’의 잎에는 Rm이 0.58인 Cu/Zn-SOD 밴드가 발현되었고, ‘재래 13호’의 경우 밴드의 밀도가 증가하는 경향을 보였다. APX 밴드 발현 양상은 두 품종 모두 적온 처리에서는 차이가 없었지만, 저온 처리 후 10일경부터 저온에 강한 ‘흑종’의 잎에서 새로운 APX 밴드가 발현되었다. POD 밴드의 발현 양상은 품종간에 뚜렷한 차이가 있었는데, 적온 하에서 ‘흑종’의 잎에서는 4개의 주된 밴드가, ‘재래 13호’의 잎에서는 한 개의 주된 밴드가 발현되었다. 그러나 저온 처리시 ‘흑종’의 잎에서는 Rm이 0.36, 0.40 및 0.54인 밴드의 밀도가 급격하게 증가한 반면 ‘재래 13호’의 잎에서는 Rm이 0.364 0.54인 밴드의 밀도가 증가하였다. 저온에 대한 내성과 관계없이 두 품종 모두 저온 처리 후 초기에는 SOD, APX 및 POD의 활성이 급격하게 증가하는 경향을 보였지만, 저온 처리 후 기간이 경과할수록 품종간 차이가 뚜렷하였다. ‘흑종’의 잎에서는 이러한 활성이 지속적으로 유지되었지만, ‘재래 13호’의 잎에서는 저온 처리 후 5일경부터 급격하게 감소하여 적온 처리구보다 낮은 활성을 보여 저온에 대한 품종간 내성 차이를 잘 반영해 주었다. To determine whether antioxidant enzyme systems are related to chilling tolerance, changes of antioxidant enzyme activities during the chilling stress were determined in the leaves of a chilling-tolerant cultivar (Cucurbita ficifolia, cv. Heukjong) and a chilling-sensitive cultivar (Cucurbita moschata, cv. Jaerae 13). Leaves of chilling-tolerant plant have two major isoforms, Fe-SOD and Mn-SOD, at the Rm values of 0.20 and 0.52, respectively. In leaves of chilling-sensitive plant, two major isozymes of SOD was observed, one isoform is Mn-SOD at the Rm value of 0.20, and the other isoform is Cu/Zn-SOD at the Rm value of 0.58. When plants were treated with chilling stress, Cu/Zn-SOD at the Rm value of 0.58 was newly expressed at 10 days after chilling stress in the chilling-tolerant plants, and density of this band increased at five days after chilling stress in the chilling-sensitive plants. One APX isozyme band was observed in unstressed plants of both cultivars. Under the chilling stress one APX isozyme band was newly expressed at 10 days after chilling stress in the chilling-tolerant cultivar. Significant genotype differences were observed for POD isozyme banding patterns such as four main isozyme bands in chilling-tolerant plants, and one band in Chilling-sensitive plants. Densities of three POD isozyme bands at the Rm of 0.36, 0.40 and 0.54 increased at 10 days after chilling stress in the chilling-tolerant plants, while two bands at the Rm of 0.36 and 0.54 increased at 10 days and 20 days after chilling stress in the chilling-sensitive plants, respectively. Activities of SOD, APX and POD significantly increased during five days after chilling stress in both cultivars. In the chilling-tolerant cultivar, activities of these enzymes were higher in chilling-stressed plant than in unstressed plants. However, activities of these enzymes in the chilling-sensitive cultivar decreased rapidly after five days of chilling stress, and were lower in chilling stressed plants than in unstressed plants.

      • KCI등재

        인터넷 이용에 따른 생활시간 패턴의 변화

        강남준(Nam-Jun Kang),이재현(Jae-Hyun Lee),오현경(Hyun-Kyung Oh) 한국언론정보학회 2008 한국언론정보학보 Vol.41 No.1

        이 연구에서는 인터넷이 일상생활에 도입되면서 전반적인 생활시간 패턴에 변화를 가져온다는 관점에서 연구문제를 설정하였다. 새로운 매체가 등장했을 때 기존의 전통적 매체 이용 시간에 대한 대체 혹은 보완 여부에만 주목할 경우, ‘미디어 고립주의’에 빠질 위험이 있다. 이뿐만 아니라 연구 결과가 일관되지 못하다는 한계점도 지적되어 왔다. 따라서 인터넷 이용 행동이 일상적 활동 영역에 미치는 영향을 거시적으로 살펴볼 수 있도록 하기 위해 2000년과 2005년도의 국민생활시간조사 자료를 통시적으로 분석함으로써 기존 연구(이재현, 2005)에서 제안된 시간 재할당 가설을 검증, 보완하고자 하였다. 먼저 연렁대 별로 인터넷 이용자와 비이용자 간에 일상적 활동 영역 및 매체 이용 영역에서 나타나는 시간 소비량의 차이를 검정한 후, 유의미한 차이를 보인 활동 영역에 대해서는 로지스틱 회구분석을 통해 인터넷 이용 유무에 따른 생활시간 이용 패턴의 차이를 종합적으로 살펴보았다. 연구 결과, 2000년도와 인터넷 사용자가 급증한 2005년도의 경우를 비교했을 때 대부분의 연령 집단에서 생활시간 패턴이 재조직화되고 있음을 확인하였으며, 특히 젊은 연령층일수록 시간 재할당 현상이 더 일찍, 더 뚜렷이 나타나는 경향을 보였다. This study tries to test time reallocation hypothesis proposed by Lee(2005) that claims the time use pattern as a whole has changed as new media is introduced - in this case, internet - in our daily life. It has been pointed out that researches, confined to study whether time use for traditional media is displaced by a new media, have such a limitation as 'media isolationism' and resulted in incoherent conclusions. In order to verify time reallocation hypothesis, which explains the influence of internet use on daily activities with broad perspectives, this research employed two different methods. First, this study analyzed the differences of time use patterns of daily activities and media use behaviors between internet users and non-users by using t-test. Second, logistic regression was used to confirm that the difference of time use pattern between internet users and non-users in many daily activities. The results show that time use pattern for daily activities has reorganized in the most of the age groups. Especially, in the young age groups, time reallocation by internet appeared more obvious than the equivalent old age groups.

      • KCI등재

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