Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

태양열 온수기의 효율향상에 대한 연구

정낙규,김진홍,오율권,김창오,강승현 조선대학교 에너지.자원신기술연구소 2002 에너지·자원신기술연구소 논문지 Vol.24 No.2

One of the ways to reduce abroad dependence for energy is to utilize solar energy when we are now confronted with not only insufficiency of natural resources but also the serious problems of environmental pollution. Therefore, full attention must now be paid to the development of solar energy system as alternative energy. To achieve this, solar hot water supply system was established at Chosun university, Gwang-ju, and experimental data were obtained based on meterological data and solar radiation. This study has been used the monthly mean values of the daily measurements obtained by data acquisition software Labview. This study shows Gwang-ju is a good region for the utilization of solar energy.

기름골 기름의 산화안정성 및 지방산조성

송진,방진기,박희운,박충범,성낙술 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

착유방법을 달리하여 얻은 기름골 기름을 37℃와 60℃에서 저장 중 산화안정성을 조사한 결과는 다음과 같다. 1. 저장 중 기름의 산가는 기간이 경과함에 따라 증가하였는데, 37℃에서 저장한 것보다 60℃에서 저장한 것이 높게 나타났고, 유기용매로 추출한 기름의 산가가 가장 높았다. 2. 저장 중 기름의 과산화물가는 37℃에서 저장한 기름의 경우 점차적인 증가를 보이다 저장 120일 이후 급격한 증가를 보이고 150일 이후 다시 감소하는 경향을 나타내었고, 60℃에서 저장한 기름은 37℃에서 저장한 기름보다 같은 저장기간에서 과산화물가 더 높게 나타났고, 그 경향은 저장 210일째까지 증가가 지속되어졌다. 3. 지방산은 oleic acid가 가장 주된 지방산으로 존재하고 있었고, 지방산은 저장기간이 경과함에 따라 palmitic acid, stearic acid, linolenic acid에서 근소한 증가와 oleic acid, linoleic acid는 약간의 감소 경향을 보였으나 저장에 따른 뚜렷한 변화는 볼 수 없었다. This study was carried out to investigate the oxidative stability and fatty acid composition during storage of chufa oil. Chufa oil was roasted at 170℃, 150℃, non-roasting or solvent extracting and stored at 37℃ or 60℃. The acid value and peroxide value of chufa oil at 60℃ were higher than their at 37℃. The acid value of chufa oil showed the trend of increase in all the samples and that of solvent-extracted oil was the highest during the storage period. The peroxide value of oils was gradually increase on the 120th day but significantly increased between 120th and 150th day. Oleic acid was the major fatty acid in chufa oil. Palmitic acid, stearic acid and linolenic acid increase and oleic acid and linoleic acid decreased during storage period. They were changed slightly and the obvious differents were not observed according to storage period.

전신성 홍반성 낭창에서의 항인지질항체검사에 관한 연구

유진우,정낙은 乙支病院 1997 乙支醫報 Vol.20 No.1

저온잠열저장을 위한 TMA-물계 포접화합물의 냉각특성에 대한 연구

김진홍,정낙규,김창오 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.2

Clathrate compound is the material that host in hydrogen bond forms cage and guest is included into it and combined. Crystallization of hydrate is generated at higher temperature than that of ice from pure water. And physical properties according to temperature are stable and congruent melting phenomenon is occurred without phase separation. But clathrate compound still had supercooling problem occurred in the course of phase change and supercooling should be minimized because it affects efficiency of equipment very much. Therefore, various studies on additives to restrain this or heat storage methods are needed. In this study was investigated the cooling characteristics of the TMA-water clathrate compound including TMA (Tri-methyl-amine, (CH₃)₃N) of 20 ~ 25 wt% as a low temperature latent heat storage material. And ethanol(CH₃CH₃OH) was added and its cooling characteristics were studied experimentally to restrain supercooling of TMA-water clathrate compound.

축냉재 포접화합물의 열물성에 관한 실험적 연구 (Ⅴ) : 온도 변화 Changing of Temperature

김진흥,정낙규 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.2

The objective of this paper is to investingate the thermal properties of TMA-clathrate applicable to cold storage system for building air-conditioning in order to use energy reasonably. Especially, the test tube experiments are performed by comparing and to analyzing the temperature of phase change, specific heat and subcooling characteristic according to the variation of concentrations and temperature of heat source in TMA clathrate. The result are summarized as follows; 1) temperature of phase change is dropped as the temperature of heat source is lower 2) specific heat of TMA-clathrate is about 40∼81 % of pure-water 3) subcooling is smalled as the temperature of heat source is lower. Thus, it apears that TMA-clathrate has thermal properties as the cold storage medium for residential air-conditioning.

저온잠열저장을 위한 포접화합물의 과냉각 특성에 대한 연구

김진흥,정낙규,김창오 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

Materials that can store low temperature solidification latent heat are organic/inorganic chemicals, eutectic salt system and clathrate compound. Clathrate compounds the material that host in hydrogen bond forms cage and guest is included into it and combined. Crystallization of hydrate is generated at higher temperature than that of ice from pure water. And physical properties according to temperature are stable and congruent melting phenomenon is occurred without phase separation and it has relatively high latent heat. But clathrate compound still has supercooling problem occurred in the course of phase change and supercooling should be minimized because it affects efficiency of equipment very much. Therefore, various studies on additives to restrain this or latent heat storage methods are needed. In this study, it was investigated the cooling characteristics of the TMA-water clathrate compound including TMA(Tri-methyl-amine, (CH3)3N) of 20∼25 wt% as a low temperature latent heat storage material. And ethanol (CH₃CH₂OH) was added and its cooling characteristics were studied experimentally to restrain supercooling of TMA-water clathrate compound.

에탄올이 첨가된 TMA포접화합물의 냉각특성

김진홍,정낙규,김창오,박호진 조선대학교 에너지.자원신기술연구소 2002 에너지·자원신기술연구소 논문지 Vol.24 No.2

The purpose of this study is to investigate the propriety of TMA clathrate as a cold storage medium. Particularly, this is to examine the extent of subcooling improvement when the additives are added to the TMA clathrate, because water used cold storage material has low phase change temperature and subcooling. This study has been analyzed and compared with TMA 30 wt% clathrate how phase change temperature, subcooling and specific heat in the various concentrations are changed. This results view to be improved phase change temperature and subcooling of TMA 30wt% as a cold storage medium, when it had some additive. Additionally, it is found that the additive must be controlled under available solution limit and study for new additive must be lasted to know its effect.

TMA-물계 포접화합물의 과냉각 억제에 대한 연구

김진홍,정낙규,김창오 朝鮮大學校 機械技術硏究所 2006 機械技術硏究 Vol.9 No.1

This study aims to find out cooling characteristics of TMA(Tri-Methyl-Amine, (CH₃)₃N) 25wt%-clathrate compound with ethanol(CH₃CH₂0H) such as supercooling, phase change temperature and specific heat. For this purpose, ethanol is added as per weight concentration and cooling experiment is performed at -6∼-8℃, cooling heat source temperature, and it leads the following result. (1) Phase change temperature is decreased due to freezing point depression phenomenon. Especially, it is minimized as 3.8 ℃ according to cooling source temperature in case that 0.5wt% of ethanol is added. (2) If 0.5wt% of ethanol is added, average supercooling degree is 0.9℃ and minimum supercooling is 0.8, 0.7℃ according to cooling heat source temperature. The restraint effect of supercooling is shown. (3) Specific heat shows tendency to decrease if ethanol is added. It is 3.013∼3.048 kJ/kgK according to cooling heat source temperature if 0.5wt% of ethanol is added. Phase change temperature higher than that of water and inhibitory effect against supercooling can be confirmed through experimental study on cooling characteristics of TMA 25wt%-water clathrate compound by adding additive, ethanol. This can lead to shorten refrigerator operation time of low temperature thermal storage system and improve COP of refrigerator and efficiency of overall system. Therefore energy can be saved and efficiency can be improved much more.

축냉재 포접화합물의 열물성에 관한 실험적 연구(Ⅱ) : 충전량 변화 Variations of Charging Conditions

김진흥,정낙규,고강옥,임재경,하옥남 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.2

본 연구에서는 에너지의 합리적 이용을 위한 건물공조용 축냉시스템에 적용 가능한 축냉재로서 TMA-물계 포접화합물의 농도와 충전량의 변화에 따른 상변화온도, 비열 및 과냉도 실험을 통하여 다음과 같은 결론을 얻었다. 1. 충전량이 많을수록 전 실헐과정에서 열물성의 안정성이 높게 나타났다. 2. TMA-물계 포접화합물의 비열은 순수한 물에 대하여 86% ∼ 96% 정도를 나타내었다. 위의 실험결과로부터 TMA-포접화합물은 건물공조용 축냉재로서의 열물성을 갖는다고 판단된다 The objective of this Paper is to investigate the thermal properties of TMA-clathrate compound applicable to cold storage system for residential air-conditioning in order to use energy reasonably. Especially, the test tube experiments are performed to compare and analyze the concentration of TMA-clathrate compound, the temperature of heat source, and the phase change temperature, specific heat and subcooling characteristics according to the variation of charge capacity. The result is summarized as follows: the phase change temperature appears higher as the charge capacity is larger. Thus, it appears that TMA-clathrate compound has thermal properties as the cold storage medium for residential air-conditioning.