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Sureshkumar P.,Selvaraj N.,Ganapathi A.,Kasthurirengan S.,Vasudevan A.,Anbazhagan V. Ramesh The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.4
Five day old cotyledon explants of Cucumber (Cucumis sativus L) cv Poinsett 76 were cocultivated with two Agrobacterium strains (EHA105 and LBA 4404) each carrying GUS as the reporter gene and npt-II as the selection marker gene in the T-DNA region of the vector. Transformed shoots were selected at 150 mg/L kanamycin. A two day cocultivation coupled with $20\;{\mu}M$ acetosyringone increased the frequency (8.2 and 15.4 shoots) of GUS expression in the shoots of transformed plant. Among the two Agrobacterium strains, EHA 105 performed better than LBA 4404 in bringing two-fold increase in transformation efficiency (14%) than LBA 4404 (7.4%). PCR analysis was done to confirm the integration of T-DNA into cucumber genome.
Aloe trinervis sp. nov.: A new succulent species from Indian Desert (Asphodelaceae)
Suresh Kumar,C.S. Purohit,Ravikiran N. Kulloli 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.2
A new species of Aloe from the Indian Desert, India, is described and illustrated as Aloe trinervis sp. nov. The new species shows similarity with Aloe vera in having succulent, rosette leaves, persistent bracts, freetepals, and six stamens but differs in the recurved teeth instead of deltoid teeth on the leaf margins, 3-nerved bracts, branched and taller inflorescences (90e95 cm long), longer flowers of 31e34 mm inlength, flowers with a pale green color being brownish at middle, and longer stamens (29e33 mm). Adetailed description with data on its distribution and relevant taxonomic notes, comparative morphological,and color photo plate are provided for easy identification of the proposed new species, A. trinervisin relation to A. vera.