꿀벌의 日週收蜜活動에 關與하는 諸要因에 關한 硏究

李明熱,吳賢宇,崔承允,禹建錫 서울대학교농과대학농업개발연구소 1989 서울대농학연구지 Vol.14 No.1

In order to employ the honeybees (Apis mellifera) profitably as insect-pollinators, the factors governing their diurnal foraging activity, the patterns of diurnal flight activity at the hive entrance and the seasonal fluctuation of colony size were examined. The results obtained are summarized as follows. 1. There was a maximum flight activity at the hive entrance between 11h and 15h during the spring and fall but it was between 08h and 09h or 16h and 17h during the summer. This activity highly correlated with the solar energy(Mj/m²) during the spring and fall. 2. The brood area and colony weight developed to the maximum in early July but total number of flying bees at the hive entrance reached the peak early in May, and decreased in June but increased again late in July. 3. The higher the degree of flowering developed, the more active the foraging behavior. Though the degree of flowering was similar in different varieties, the foraging preference in the apple blossoms differed with varieties. 4. The amount of nectar per flower was variable with time hours, age of flowers and plant varieties. 5. The number of honeybees collecting only the nectar in the apple blossoms increased in the afternoon. Foraging behavior in the pear blossoms changed with the varieties and the numbers of honeybees collecting only the pollen (66%) or only the nectar (31%) did not change with time in the peach blossoms. 6. The diurnal foraging activities of honeybees on eight honey plants highly correlated with the solar energy but temperature, relative humidity and wind speed were not.

Antioxidant capacity of chestnut (Castanea crenata Sieb. et Zucc) honey produced in Korea

Hye-Kyung Kim,Myeong-Lyeol Lee,Man-Young Lee,Yong-Soo Choi,Nam-Suk Kim,In-Pyo Hong,Kwang Gill, Lee,Byung Rae Jin 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

This study aimed to evaluate the antioxidant activity of chestnut honey which were harvested at various areas in South Korea. First at all, we measured the total phenols content through a spectrophotometric determination with a modified Folin-Ciocalteu method and total flavonoids content determined with aluminium chloride. Total phenolic compounds was highest in Sunchang of Chestnut honey(2.21mg/ml)and flavonoids contents was also the highest in Sunchang of Chestnut honey(1.02mg/ml) than other samples. For measured the antioxidant activity of chestnut honey, we performed DPPH(2,2-diphenyl-1-picrylhydrazyl) test and FRAP(ferric reducing-antioxidant assay)test. DPPH scavenging activity highest in Sunchang of Chestnut honey more than 50% DPPH scavenging activitywhile other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 25% DPPH scavenging activity. The ferric reducing-antioxidant assay (FRAP) is based on the reduction of ferric 2,4,6-tris(2-pyridyl)-1,3,5-triazine [Fe(III)-TPTZ] by spectrophotometric analysis. Sunchang were found to have more than 532μM FRAP activity while other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 300μM FRAP activity. The results suggested that chestnut honey strong antioxidant activity and it could be utilized as a source of natural antioxidant.

Detection of sacbrood virus (SBV) from the honeybee in Korea

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Sacbrood virus(SBV) causes a fatal disease(sacbrood) of honeybee larvae, which fail to pupate, change color and shape, and finally die. The complete nucleotide sequence of SBV has recently been determined, and with these data, we now report a Reverse Transcription-PCR(RT-PCR) test for the direct, rapid, and sensitive detection of these viruses. To detect the SBV infection in Korea, we collect beekeepers from various apiaries, which the RT-PCR technique was used. And we designed SBV specific primers in conserved region of the viral genome in the GenBank database. We confirmed the SBV amplicon using cloning and sequence. Homology between determined sequences of SBV korean strain and published virus sequences were 97% in DNA sequence, and 100% in amino acid sequence. We describe the first time that presence of sacbrood virus(SBV) in Korea honey bee colonies using RT-PCR. We also developed and validated a RT-PCR assay for the detection of SBV in Korea.

Occurrence of seven honeybee viruses in Korean apiaries

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Mortality of honeybees is a serious problem that beekeepers have to face periodically in Korea and worldwide. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. In this work, we detected Deformed wing virus(DWV), Israle Acute Paralysis Virus (IAPV), Black queen cell virus (BQCV), Cloudy wing virus(CWV), Kashmir bee virus(KBV), Sacbrood virus(SBV), Chronic bee paralysis virus(CBPV) in samples of korea honeybees with or without Varroa destructor and Nosema apis. The detection of viruses in all provinces, simultaneous co-infection of colonies by several viruses and the fact that 96.3% of the samples were infected with one or more virus, indicates they are widely spread in the region. Using uniplex and multiplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including DWV, IAPV, BQCV, KBV, CWV, and described the detection of mixed virus infections in bees from these colonies. Conclusively, investigated disease of the bee, and confirmed new virus that lead to bee disease, this is thought by valuable thing as data for development of beekeeping industry such as CCD(Colony Collapse Disorder)'s cause searching examination.

Detection of chronic bee paralysis virus (CBPV) by minus-strand-specific RT-PCR

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Viruses of the honeybee, Apis mellifera L. are known to reside at low levels in colonies, typically showing no apparent signs of infection. Chronic paralysis virus(CBPV) is known to induce significant losses in honey bee colonies. The pathology is characterized by clusters of trembling, flightless, crawling bees and by individual bees, sometimes hairless, standing at the hive entrance. A minusstrand-specific RT-PCR was used to assess viral replication. This is the first report on the infection of CBPV in Korea. Using (-)RT-PCR, 27 apiaries in korea were screened for the honeybee viruses, with positive colonies being analysed for viral genetic diversity. We got 550-nt PCR product from CBPV genomic RNA. Nucleotide sequences were aligned to the complete CBPV genomic RNA sequence deposited in the GenBank database and was revealed 96%(AM-CBPV) identity, respectively. Sequence comparison with other CBPV and honeybee virus.

Effect of Climate Change on Life Span and Disease Susceptibility in Honey Bees, Apis mellifera L.

Myeong-Lyeol Lee,Man-young Lee,Gyu-Ho Byeon,Yong-Soo Choi,Hye-Kyung Kim 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.3

Distribution and control strategies of honeybee parasitic mites, Varroa destructor Anderson & Trueman, in South Korea

Myeong-Lyeol Lee,Yong Soo Choi,Won Tae Kim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Varroa destructor Anderson & Trueman is the most injurious parasitic pest of honeybee in the world. Varroa mites had been originally external parasites of Asian honeybee (Apis cerana Fab.) in south eastern Asia. They jumped to European honeybees (Apis mellifera L.) by 1963. Since then they have killed millions of European honeybee colony, which might be susceptible to them, in Asia, Europe, America, and Africa. Also in Korea since Varroa mites were first found in 1968, they have been destructive pests in most of A. mellifera apiaries. Varroa destructor commonly infesting the European honeybees was classified in 2000 as a different species from the Varroa jacobsoni originally identified on Asian honeybees. Varroa mites not only feed the haemolymph of bees, but also introduce virulent viral diseases, and interrupt the development of bee colony. The other external parasitic mite, Tropilaelaps clarea Delfinado & Baker, which was introduced in 1994 from China, has widely spread and also brought damages on honeybees.

미토콘드리아 DNA 증폭을 이용한 한국의 잎응애속(Tetranychus, Acarina: Tetranychidae) 4종의 동정방법

Myeong-Lyeol LEE,Mun-Hong LEE 한국응용곤충학회 1997 한국응용곤충학회지 Vol.36 No.1

미국과 캐나다는 한국에 분포하는 잎응애속(Tetranychus)중 범세계적 분포종인 점박이응애(T. urticae Koch)를 제외한 벚나무응애(Tetranychus vienensis Zacher), 차응애(T. kanzawai Kishida), 뽕나무응애(T. truncatus Ehara)를 검역대항으로 하고 있다. 잎응애속 응애들은 암컷성충으로 월동휴면에 들어가는데 기존의 수컷생색기의 형태를 위주로 한 동정방법으로는 이 휴면태의 암컷을 정확히 동정하기 어렵다. 월동을 위해 사과 과실 꼭지부에 우발적으로 부착할 가능성이 있는 것으로 우려되는 잎응애속 응애들의 월동휴면태에 대한 신속, 정확한 동정법이 수출검역현장에서 절실히 요구되는 실정이다. 사과의 주요해충인 점박이응애와 과수원 주변에서 발견되는 벚나무응애, 뽕나무응애, 차응애의 미토콘드리아 DNA(mtDNA)내 cytochrome oxidase subunit I(CO-I) 유전자를 PCR로 증폭하고 증폭된 DNA의 종간 변이를 이용하여 발육영기나 암수에 관계없이 동정할 수 있는 방법을 찾는 연구를 수행하였다. 세쌍의 primer에 의해 미토콘드리아 DNA의 CO-I 유전자 일부(680 bp)를 중복되게 증폭하였고 증폭된 유전자는 제한효소 AluI, DdeI, Sau3A 대하여 응애종간 특이적 인식부위를 가지고 있었다. 제한효소에 의해 절단되는 특이적 DNA 단편은 Tetranychus 응애류를 동정하는데 유용한 표식인자로 사용될 수 있을 것이다. 아울러 증폭한 CO-I 유전자내의 제한효소 인식부위에 대한 이들 4종 응애의 유전자지도를 작성하였다. Except for a cosmopolitan and major pest of apples, Tetranychus urticae Koch, Tetranychus mites in Korea such as T. viennensis Zaher, T. kanzawai Kishida, and T. truncams Ehara have been considered as quarantine pests by Canada and United States. Even though these mites are not feeders on apples, they are suspected to attach accidentally on apple h i t s in autumn as females enter the diapause. The characters used to identify Tetranychus mites have been confined to the shape of aedeagus in adult male. To develope a fast and accurate alternative identification protocol applied to hibernating female mites on apples, their mitochondrial DNA (mtDNA) were examined to find out any polymorphisms to discriminate each species from the other ones. Three pairs of primers for polymerase chain reaction (PCR) were used to amplify cytochrome oxidase subunit I (CO-I) coding region in mitochondrial DNA5 of four species of Tetranycus mites. The longest amplified product was estimated its size as about 680 bp. Digestion with restriction enzymes, AluI, Ddel, and Sau3A, showed length polymorphisms, which will he useful as diagnostic markers to identify Tetranychus mites. Schematic restriction maps in amplified region were shown for each species.

Existence of Apis mellifera L. Spermatozoa in the Spermathecae of Apis cerana Fab. Reared in the Region Highly Populated by A. mellifera

Lee Myeong-Lyeol,Park Young-Mi 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

Selection of Triple Crossed Honeybee (Apis mellifera L.) Enhanced Honey Production

Myeong-lyeol Lee,Man-young Lee,Yong-soo Choi,Hye-kyung Kim,In-suk Kim,Hyo-yeol Choi 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.3