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Chae, Keon-Sang,Katsuji Murakawa,Kousaku Okubo,Kenichi Mastsubara 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
For the PCR-based chromosomal assignment of very short cDNA fragments specifically designed primers are required. We tested primers with very short core sequences that are identical or complementary to known cDNA sequences, with or without tails at the 5' ends. The lower limit of the core length for PCR using human chromosome templates was 14 nucleotides (nt) when they have tails. The minimal length of the tail was 2 nt when it was attached to the 5' end of a 14-nt core. In the absence of a tail. 15 nt are needed for the core to act properly. The overall size of the short cDNA fragments that could be assigned was further reduced by using a pair of primers that overlap at the 3' ends. The limits of the free energy of overlap were about -1.9 kcal mol at 45 C, -2.9 ㎉/mol at 50 C and -4.5 ㎉/mol at 55 C. A combination of these features in a primer pair allowed cDNA fragments as short as 30 nt to be assigned.
Chromosomal Assignment of Short cDNA Sequences by PCR Using Overlapping and Tailed Short Primers
CHAE, Keon-Sang,MURAKAWA, Katsuji,OKUBO, Kousaku,MATSUBARA, Kenichi 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
Overlapping primers and tailed short primers are effective agents for mapping very short cDNA sequences. By using such primers, human cDNAs as short as 32 nucleotides in length can produce PCR bands. Using these and other primers of ordinary size, 44 cDNAs were assigned to chromosomes, of which 24 were assigned to single chromosomes, and 2 were assigned to two chromosomes and two were assigned to three chromosomes, respectively. Among the 24 cDNAs, all of which matched GenBank entries, 6 cDNAs were observed to map to the same chromosomes as reported previously.
( Masumi Hasegawa ),( Hiroyuki Okamura ),( Mami Murakawa ),( Kazutoshi Takeuchi ),( Keiichi Yamada ),( Kiyotaka Uchikura ),( Noriyuki Harada ),( Junji Matsumura ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1
Some fire-retardant woods with no documented fire performance have occasionally been sold in the market. It is important to know whether the required amount of chemical fire retardant is present in all sections of a wood sample. In this study, nondestructive measurement of chemical retention in fire-retardant treated wood was experimentally investigated using air-coupled ultrasonic and micro focus X-ray computed tomography. Wood samples were impregnated with different chemical concentrations. First, the ultrasonic wave velocities of wood samples were measured before and after impregnation. The percent changes in velocity exhibited a positive correlation with the chemical retention. Second, the images of wood sample were captured using X-ray CT before and after impregnation. The changes in integrated brightness values calculated from the captured images also showed a positive correlation with the chemical retention at a 1% significance level. These findings suggest that it is possible to evaluate the distributions of chemical retention in fire-retardant wood.