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한국, 중국 및 엘살바도르 강낭콩 (Phaseolus vulgaris L.) 유전자원의 농업 특성과 다양성 비교
최유미(Yu-Mi Choi),이수경(Sugyeong Lee),현도윤(Do yoon Hyun),윤문섭(Munsup Yoon),오세종(Sejong Oh),이명철(Myung-Chul Lee),이정로(Jeongro Lee),고호철(Hocheol Ko),허온숙(Onsook Huh) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
This study was conducted to compare the agricultural characteristics of total 444 common bean accessions from Korea(296), China (76), and El Salvador (72). Days to flowering were ranged from 41 to 83 days with an average of 61 days. Days to sowing to maturing were ranged from 86 to 143 days with an average of 104 days. Common beans from El Salvador tend to bloom and mature 3 to 7 days earlier than Korea and China accessions. In growth habit, over 50% of Korea and China accessions were indeterminate and climbing type, but 90.1% from El Salvador were Semi-determinate and climbing type. Qualitative traits were much different among three countries. Eighty-two percentage of immature pod colors were dark pink from El Salvador, but many of those were pale yellow from Korea (77.6%) and China (61.8%). Seed shapes were divided into four types of round, oval, cubic and kidney type, and the highest percentage of those were 30.4% with kidney type. The highest of those was 36.6% with oval type from Korea, 55.3% with kidney type from China and 79.2% with cubic type from El Salvador. Morphological characteristics of common bean from El Salvador were much different from those of Korea and China, which is necessary to collect more germplasm from its native and expand genetic diversity of common beans. Four hundred thirty-five common beans from Korea, China and El Salvador were analyzed using SSR markers. Ninety-two alleles were detected with a lowest of 6 at the BM161, BM181 and a highest of 18 at the BM154, BM160. The average polymorphism information content(PIC) was 0.72. To similar with population size among three countries, 292 Korean accessions divided four replications with 73 accessions. As a result genetic diversity was the highest of 0.73 in Chinese populations, while the lowest of 0.48 in El Salvador populations.
Young-Yi Lee,Sun-Yi Lee,Jae-Young Song,Munsup Yoon,Jung-Yoon Yi,Jung-Ro Lee,Haeng-Hoon Kim 한국자원식물학회 2020 한국자원식물학회지 Vol.33 No.6
This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions ‘Massey’ and ‘MDUS3816’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 ㎝× 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.
배진주(Jinjoo Bae),이선이(Sun-Yi Lee),송재영(Jae-Young Song),이정로(Jung-Ro Lee),윤문섭(Munsup Yoon),이정윤(Jung-Yoon Yi),김행훈(Haeng-Hoon Kim),이영이(Young-Yi Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6
Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).
Jinjoo Bae,Young-Yi Lee,Sun-Yi Lee,Jae-Young Song,Bong-Nam Chung,Jung-Ro Lee,Munsup Yoon,Jung-Yoon Yi,Haeng-Hoon Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Strawberry (Fragaria × ananassa Duch.) is one of the most important crops in many countries because of its taste and nutrients. Several reports raised the concerns of somaclonal variations by tissue cultures which is the major propagation methods for strawberry productions. In order to prevent the issue, studies suggested using low concentrations of cytokinins and a reduced number of subcultures during in vitro propagation. For a long term preservation of genetic resources, cryopreservation technique has been developed on strawberry germplasm and mainly used in vitro grown shoot tips. In this study, genetic and morphological stability of six strawberry accessions such as ‘Pink paend’, ‘Gorella’, ‘NY1406’, ‘Merrimack’, ‘Tangi’ and ‘Tufts’ were tested under treatment conditions, 1) conventional propagation (Control), 2) In vitro propagation (tissue culture, TC), 3) non-liquid nitrogen treatment (pre-treatment before cryopreservation, LNC), and 4) cryopreservation (liquid nitrogen treatment, LN). To examine genetic stability, 15 inter simple sequence repeat (ISSR) primers were used. 12 out of 15 primer sets were selected based on their reproducibility of band patterns. A total of 102 bands were generated and no polymorphism were found among four treatments and three replications within an accession except one cultivar. A few polymorphic bands were found in only tissue cultured plants (TC) of “Tufts”. To test morphological variations, rooted plants under in vitro grown, were planted in greenhouse after acclimatization. We measured vegetative traits including plant length, leaf length, leaf width, petiole length and number of runners. There are no significant differences in these traits among treatments within an accession. This study demonstrates that the cryopreserved plants of the six strawberry accessions are genetically and morphologically stable.