http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yoon, Moongeun,Jung, Ju-Yeon,Kim, Dong Soo The Korean Society of Fisheries and Aquatic Scienc 2013 Fisheries and Aquatic Sciences Vol.16 No.4
Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population.
Woo, Seonock,Yoon, Moongeun,Suh, Dong-Sang,Song, Jun-Im,Kim, Jeong Ha,Yum, Seungshic 한국유전학회 2004 Genes & Genomics Vol.26 No.4
The complete cDNA of eukaryotic translation elongation factor 1A (eEF1A) from the soft coral Scleronephthya gracillimum (Ku¨kenthal) (Alcyonacea, Octocorallia, Anthozoa, Cnidaria) was cloned by RT-PCR and the nucleotide sequences were determined. The information on primary structure of the eEF1A gene and protein may be useful for the gene expression study as well as for the phylogenetic study. The full-length cDNA of S. gracillimum eEF1A comprised 1612 bp, not including the poly (A)^+ stretch, and contained a complete open reading frame encoding 461 amino acids. The deduced amino acid sequence showed 85%identity to placozoan, 86% to human and 77% to hydra. Considering that functional domains are highly conserved throughout the evolutionary history, the present study suggests that the amino acid sequence of eEF1A alone may not resolve the phylogenetic relationship of lower metazoans. A possible solution to overcome the long branch problems that appear in cnidiarian phylogeny is discussed.
문일윤(Il-Yoon Moon),문인상(Insang Moon),유재한(Jaehan Yoo),전재형(Jae-Hyoung Jeon),이선미(Seon-Mi Lee),홍문근(Moongeun Hong),하성업(Seong-Up Ha),강상훈(Sang Hun Kang),이수용(Soo Young Lee) 한국추진공학회 2011 한국추진공학회 학술대회논문집 Vol.2011 No.11
다단연소 사이클 로켓엔진용 산화제과잉 예연소기 연소성능 평가를 위해 점화시험을 수행하였다. 산화제과잉 예연소기는 혼합비 60, 20 ㎫의 연소압에서 작동하도록 설계되었다. 케로신과 액체산소의 일부는 혼합헤드를 통해 연소실로 공급되어 산화제과잉 환경에서 연소되며 나머지 액체산소는 연소실 중앙에 위치한 분사구를 통해 연소실에 주입되어 기화된다. 접촉발화성 연료로 별도의 점화용 분사기 없이 전체 분사기를 통해 점화용 추진제를 공급하여 점화하는 방식을 사용하였다. 안정적 점화를 위해 각각의 추진제를 2단으로 공급하여 점화할 수 있도록 하였다. 시험결과 설계유량의 45% 이하의 저유량 점화구간에서 저주파 진동이 발생하였다. 저주파 진동을 피하기 위해 저유량 구간을 최소화하는 방식으로 설계 연소압까지 안정적 점화를 유도할 수 있었다. Ignition tests of an oxidizer rich preburner for a staged combustion cycle liquid rocket engine were performed to evaluate combustion performance. Design operation conditions of the tested oxidizer rich preburner are about 60 of OF ratio and 20 ㎫ of combustion pressure. The entire kerosene and some LOx injected into the mixing head is burned in combustion chamber and the remaining LOx injected through center holes of combustion chamber is vaporized. Full flow ignition method with hypergolic fuel was used. Each propellant was supplied in two stages for soft ignition. Test results, low frequency oscillation was occurred in low flow rate conditions under 45% of design flow rate. Stable ignition in the course of design combustion pressure was able to induce by minimization of low flow rate ignition region to escape low frequency oscillation.
Chang Ho Yi,Moongeun Yoon,Ji Min Kim,Il‑Hun Kim,In‑Young Cho,Hye Suck An 한국유전학회 2021 Genes & Genomics Vol.43 No.6
Background Mytilus coruscus Gould, 1861 is a mussel species in the family Mytilidae, native to the Northwest Pacifc Ocean, ranging from the East China Sea, the Yellow Sea, and as far as the Peter the Great Gulf in the East Sea. In Korea, this species has been heavily exploited for nutrient-rich food resources and experienced severe reduction in their population. Objective The aim of this study was to investigate the genetic diversity and population structure and to provide baseline data to facilitate the conservation and sustainable use of the vulnerable species M. coruscus in South Korea. Methods Mitochondrial DNA (mtDNA) cytochrome c oxidase I (COI) sequences of 91 adult individuals from four islands and one coastal localities in South Korea were sequenced. We then compared genetic diversity and haplotype data with previously published Chinese wild populations. Results Mytilus coruscus populations on Korean coasts were found to exhibit high genetic diversity despite concerns regarding recent population reduction. A total of 42 haplotypes were defned by 56 polymorphic sites. High-level genetic diversity was observed on four Island sites (Hd=0.906–0.955, π=0.0068–0.0090). The other seashore site represented relatively lower genetic diversity (Hd=0.529, π=0.0011) and was genetically diferentiated from the others. In a previous study, wild populations on the East China Sea exhibited similarly high genetic diversity as that observed in our study. Additionally, Chinese M. coruscus populations exhibit a distinct regional haplotype distribution pattern while sharing six haplotypes with Korean populations. Conclusions The results of this study provide insights that further the current understanding regarding the evolution of M. coruscus species and provides comprehensive genetic data to facilitate the development of an efective conservation strategy.
Lee, Sang Beum,Kim, Yong Soo,Yoon, Moongeun,Kim, Su-Kyoung,Jang, In Kwon,Lim, Hyun Jeong,Jin, Hyung-Joo The Korean Society for Marine Biotechnology 2007 한국해양바이오학회지 Vol.2 No.4
Muscle tissue expresses many muscle-specific genes, including myostatin (also known as GDF8) that is a member of the transforming growth factor-beta superfamily. Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. In this study, we first have characterized partial cDNA of a MSTN gene from the muscle tissue in the F. chinensis and examined its expression pattern in various tissues. The partial MSTN gene (GenBank accession number EU 131093) in the F. chinensis was 1134 bp, encoding for 377 amino acids that showed 63-93% amino acid similarity to other vertebrate MSTNs, containing a conserved proteolytic cleavage site (RXRR) and conserved cysteine residues in the C-terminus. Based on a RT-PCR, the MSTN gene was expressed in the all tissues of F. chinensis used in this study.
Kang, Nam Seon,Lee, Jung A,Jang, Hyeong Seok,Kim, Kyeong Mi,Kim, Eun Song,Yoon, Moongeun,Hong, Ji Won Korean Society of Environmental Biology 2019 환경생물 : 환경생물학회지 Vol.37 No.4
Chlorella gloriosa (Chlorellaceae, Trebouxiophyceae) was isolated from seawater off the coast of the Dokdo Islands in Korea. An axenic culture was established using the streak-plate method on f/2 agar media supplemented with antibiotics, allowing identification of the isolate by morphological, molecular, and physiological analyses. The morphological characteristics observed by light and electron microscopy revealed typical morphologies of C. gloriosa species. The molecular phylogenetic inference drawn from the small-subunit 18S rRNA sequence verified that the microalgal strain belongs to C. gloriosa. Additionally, gas chromatography-mass spectrometry analysis showed that the isolate was rich in nutritionally important omega-3 and -6 polyunsaturated fatty acids and high-performance liquid chromatography analysis revealed that the high-value antioxidants lutein and violaxanthin were biosynthesized as accessory pigments by this microalga, with arabinose, galactose, and glucose as the major monosaccharides. Therefore, in this study, a Korean marine C. gloriosa species was discovered, characterized, and described, and subsequently added to the national culture collection.
Characterization of MABIK Microalgae with Biotechnological Potentials
Seung-Woo Jo,Nam Seon Kang,Jung A Lee,Eun Song Kim,Kyeong Mi Kim,Moongeun Yoon,Ji Won Hong,Ho-Sung Yoon 한국해양바이오학회 2020 한국해양바이오학회지 Vol.12 No.1
This article emphasized the physiological characteristics of the selected marine microalgal strains obtained from the culture collection of the National Marine Biodiversity Institute of Korea (MABIK). Therefore, in this study, 13 different marine microalgal strains belonging to the phylum Chlorophyta were analyzed for the composition of fatty acids, elements, photosynthetic pigments, and monosaccharides, as well as the lipid and protein contents. The results presented that the primary fatty acids were palmitic (C<SUB>16:0</SUB>), palmitoleic (C<SUB>16:1</SUB> n-7), stearic (C<SUB>18:0</SUB>), oleic (C<SUB>18:1</SUB> n-9), linoleic (C<SUB>18:2</SUB> n-6), and α-linolenic (ALA, C<SUB>18:3</SUB> n-3) acid in the evaluated microalgae. The lipid contents of heterotrophically grown strains ranged from 15.1% to 20.4%. The calorific values of the strains were between 17.4 MJ kg<SUP>-1</SUP> and 21.3 MJ kg<SUP>-1</SUP>. The major monosaccharides were galactose, glucose, and mannose, while the primary photosynthetic pigments were chlorophyll-a (Chla), chlorophyll-b (Chlb), and lutein, respectively. Based on the results, the microalgal strains showed high potentials in the use of microalgae-based technologies to produce biochemicals, food, and renewable fuels as they are rich in sustainable sources of high-value bio-compounds, such as antioxidants, carbohydrates, and fatty acids.