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RISS 인기검색어
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- 저자 : Moghaddamnia, Seyed Hassan (검색결과 3 건)
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Rezaei-Tavirani, Mostafa,Moghaddamnia, Seyed Hassan,Ranjbar, Bijan,Amani, Mojtaba,Marashi, Sayed-Amir Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.5
Thermal conformational changes of human serum albumin (HSA) in phosphate buffer, 10 mM at pH = 7 are investigated using differential scanning calorimetric (DSC), circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature increment from $25^{\circ}C$ to $55^{\circ}C$ induces reversible conformational changes in the structure of HSA. Conformational change of HSA are shown to be a three-step process. Interestingly, melting temperature of the last domain is equal to the maximum value of fever in pathological conditions, i.e. $42^{\circ}C$. These conformational alterations are accompanied by a mild alteration of secondary structures. Study of HSA-SDS (sodium dodecyl sulphate) interaction at $45^{\circ}C$ and $35^{\circ}C$ reveals that SDS affects the HSA structure at least in three steps: the first two steps result in more stabilization and compactness of HSA structure, while the last one induces the unfolding of HSA. Since HSA has a more affinity for SDS at $45^{\circ}C$ compared to $35^{\circ}C$, It is suggested that the net negative charge of HSA is decreased in fever, which results in the decrease of HSA-associated cations and plasma osmolarity, and consequently, heat removal via the increase in urine volume.
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Javad Saien,Farzaneh Moghaddamnia,Mokhtar Mishi 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.5
The simultaneous influence of NaCl, KCl and KI salts and well known sodium dodecyl sulfate (SDS) surfactant on the interfacial tension (IFT) of conventionally used chemical system of toluene-water was studied. The concentration range of salts was within (0.010 to 0.075) mol/dm3 and of surfactant within (1.7 to 26.0)×10−5 mol/dm3. SDS adsorption on interface is highly intensified in the presence of salts and IFT can reach to 67.1% of its initial value. Accordingly,the role of ions was investigated and the order of salts effectiveness was revealed as KI>>KCl>NaCl. The obtained data, with both effects, were nicely reproduced using an equation of state, based on Gibbs adsorption equation and the Langmuir isotherm. Two relevant important adsorption parameters exhibited reasonable variations. Furthermore,the general revealed linear variation of IFT with a previously defined “effective concentration” indicates the strong influence of the surfactant counterions.
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