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      • KCI등재

        Effect of CBN surface coating on properties of abrasive and vitrified CBN composites

        Xuezhi Wang,Minghai Wang,Jingqiang Zhang,Wenbo Fan,Tianbiao Yu 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.1

        The advantages of CBN abrasive and vitrified bonds allow the wide use of vitrified CBN grinding tools. However, theirproperties can be affected by various factors such as vitrification itself and binding between vitrified bond and abrasive grains. In view of this, the current work aims to improve the binding characteristics of vitrified bond and abrasive grains byelucidating the effect of CBN surface coating. In particular, the thermal properties of typical nickel and titanium coatedabrasive grains were compared with those of ordinary abrasive grains. Furthermore, the strength, microstructure and phasecomposition of vitrified CBN composites were studied as well. According to the results, the thermal stability of abrasive grainsdecreased after coating processing. However, applying the vitrified CBN composite material and sintering, if appropriable, wasshown to ensure protection of the abrasive grains. Moreover, as an intermediate transition layer, the bonding properties ofvitrified bond and abrasive grains can be improved to some extent. Finally, the vitrified Ti-coated CBN composite was foundto possess the best performance.

      • KCI등재

        Activation of the Unfolded Protein Response via Co-expression of the HAC1i Gene Enhances Expression of Recombinant Elastase in Pichia pastoris

        Minghai Han,Weixian Wang,Jianli Zhou,Xun Gong,Cunbin Xu,Yinfeng Li,Qiang Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2

        The effects of activation of the unfolded protein response (UPR) via co-expression of the HAC1i gene on the production of the recombinant Pseudomonas aeruginosa elastase (rPAE) in Pichia pastoris GS115 were evaluated in this study. The results showed that expression of the HAC1i gene significantly increased the level of Kar2p (a hallmark of UPR activation) in P. pastoris GS115, demonstrating activation of the UPR. This gene did not affect the growth of yeast in the buffered glycerol-complex medium but stimulated its growth in the buffered methanolcomplex medium. Co-expression of the HAC1i gene enhanced the expression level of the heterogeneously Nglycosylated forms of rPAE, as the caseinolytic activity in the supernatant of the various glycoforms of rPAE expressed in P. pastoris GS115/HAC1 was increased 1.8—3.9-fold compared to that in the control strain P. pastoris GS115, respectively. The stimulating effects of co-expression of the gene on rPAE production were observed when 0.5, 1.0, and 2.0% methanol were added every 24 h, as the caseinolytic activity of supernatants of P. pastoris GS115/HAC1 expressing wild-type of rPAE was increased 3.3-, 1.9-, and 1.7-fold at the corresponding methanol concentration. Further, activation of UPR via co-expression of the HAC1i gene enhanced rPAE secretion in P. pastoris at 20, 24, and 28°C, as the caseinolytic activity of supernatants of P. pastoris GS115/HAC1 expressing wild-type rPAE was increased 2.3-, 2.1-, and 2.8-fold over the tested temperatures.

      • KCI등재

        Deep Learning: High-quality Imaging through Multicore Fiber

        Liqing Wu,Jun Zhao,Minghai Zhang,Yanzhu Zhang,Xiaoyan Wang,Ziyang Chen,Jixiong Pu 한국광학회 2020 Current Optics and Photonics Vol.4 No.4

        Imaging through multicore fiber (MCF) is of great significance in the biomedical domain. Although several techniques have been developed to image an object from a signal passing through MCF, these methods are strongly dependent on the surroundings, such as vibration and the temperature fluctuation of the fiber’s environment. In this paper, we apply a new, strong technique called deep learning to reconstruct the phase image through a MCF in which each core is multimode. To evaluate the network, we employ the binary cross-entropy as the loss function of a convolutional neural network (CNN) with improved U-net structure. The high-quality reconstruction of input objects upon spatial light modulation (SLM) can be realized from the speckle patterns of intensity that contain the information about the objects. Moreover, we study the effect of MCF length on image recovery. It is shown that the shorter the fiber, the better the imaging quality. Based on our findings, MCF may have applications in fields such as endoscopic imaging and optical communication.

      • KCI등재

        Effect of bond slip on the performance of FRP reinforced concrete columns under eccentric loading

        Chunyang Zhu,Li Sun,Ke Wang,Yue Yuan,Minghai Wei 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.1

        Concrete reinforced with fiber reinforced polymer (FRP) bars (FRP-RC) has attracted a significant amount of research attention in the last three decades. Alimited number of studies, however, have investigated the effect of bond slip on the performance of FRP-RC columns under eccentric loading. Based on previous experimental study, a finite-element model of eccentrically loaded FRP-RC columns was established in this study. The bondslip behavior was modeled by inserting spring elements between FRP bars and concrete. The improved Bertero-Popov-Eligehausen (BPE) bond slip model with the results of existing FRP-RC pullout tests was introduced. The effect of bond slip on the entire compression-bending process of FRP-RC columns was investigated parametrically. The results show that the initial stiffness of bond slip is the most sensitive parameter affecting the compression-bending performance of columns. The peak bond stress and the corresponding peak slip produce a small effect on the maximum loading capacity of columns. The bondslip softening has little effect on the compression-bending performance of columns. The sectional analysis revealed that, as the load eccentricity and the FRP bar diameter increase, the reducing effect of bond slip on the flexural capacity becomes more obvious. With regard to bond slip, the axial-force-bendingmoment (P-M) interaction diagrams of columns with different FRP bar diameters show consistent trends. It can be concluded from this study that for columns reinforced with large diameter FRP bars, the flexural capacity of columns at low axial load levels will be seriously overestimated if the bond slip is not considered.

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