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- 저자 : Mingchun Wang (검색결과 2 건)
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Growth Inhibitory Effect of Mangiferin on Thyroid Cancer Cell Line TPC1
Lei Zhang,Mingchun Wang 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.6
Thyroid cancer is the most frequently occurring cancer in human. Therefore, there is a need for better understanding and improvement in the treatment for thyroid tumorigenesis. Many studies show that natural dietary components like Mangiferin (present in mangoes) have anti-carcinogenic ability and reduce the incidence and development of cancers. The present study was focused on analysing the effect of Mangiferin on human thyroid cancer cell line (TPC1) and to assess its therapeutic significance against aggressive thyroid cancers. The cell viability of TPC1 cells was evaluated by MTT assay. The DAPI and AO/Er staining using fluorescence microscopy was used to evaluate the effects of the compound on apoptosis. The PCNA expression was also examined. In the present study, Mangiferin has significantly inhibits TPC1 cell proliferation. DAPI nuclear staining revealed apoptotic changes occurred in nuclei of cells treated with mangiferin (4 μM). AO/EtBr staining of TCP-1 cells showed that mangiferin treatment (2 and 4 μM) induced cell death. Mangiferin induces apoptosis through the induction of cas-3 and reduced Bcl-2 expression. It was concluded that mangiferin triggered the apoptotic pathways. It also decreased the viability of TPC-1 cells by suppressing the PCNA.
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Ran An,Yongli Guo,Mingchun Gao,Junwei Wang 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.5
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) on the surface of Streptococcus dysgalactiae, coded with gapC, is a glycolytic enzyme that was reported to be a moonlighting protein and virulence factor. Objective: This study assessed GAPDH as a potential immunization candidate protein to prevent streptococcus infections. Methods: Mice were vaccinated subcutaneously with recombinant GAPDH and challenged with S. dysgalactiae in vivo. They were then evaluated using histological methods. rGAPDH of mouse bone marrow-derived dendritic cells (BMDCs) was evaluated using immunoblotting, reverse transcription quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay methods. Results: Vaccination with rGAPDH improved the survival rates and decreased the bacterial burdens in the mammary glands compared to the control group. The mechanism by which rGAPDH vaccination protects against S. dysgalactiae was investigated. In vitro experiments showed that rGAPDH boosted the generation of interleukin-10 and tumor necrosis factor-α. Treatment of BMDCs with TAK-242, a toll-like receptor 4 inhibitor, or C29, a toll-like receptor 2 inhibitor, reduced cytokines substantially, suggesting that rGAPDH may be a potential ligand for both TLR2 and TLR4. Subsequent investigations showed that rGAPDH may activate the phosphorylation of MAPKs and nuclear factor-κB. Conclusions: GAPDH is a promising immunization candidate protein for targeting virulence and enhancing immune-mediated protection. Further investigations are warranted to understand the mechanisms underlying the activation of BMDCs by rGAPDH in a TLR2- and TLR4-dependent manner and the regulation of inflammatory cytokines contributing to mastitis pathogenesis.
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