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Application of high-resolution melting analysis for differentiation of spoilage yeasts
Mine Erdem,Zülal Kesmen,Esra Özbekar,Bülent Çetin,Hasan Yetim 한국미생물학회 2016 The journal of microbiology Vol.54 No.9
A new method based on high resolution melting (HRM) analysis was developed for the differentiation and classification of the yeast species that cause food spoilage. A total 134 strains belonging to 21 different yeast species were examined to evaluate the discriminative power of HRM analysis. Two different highly variable DNA regions on the 26 rRNA gene were targeted to produce the HRM profiles of each strain. HRMbased grouping was compared and confirmed by (GTG)5 rep- PCR fingerprinting analysis. All of the yeast species belonging to the genera Pichia, Candida, Kazachstania, Kluyveromyces, Debaryomyces, Dekkera, Saccharomyces, Torulaspora, Ustilago, and Yarrowia, which were produced as species-specific HRM profiles, allowed discrimination at species and/or strain level. The HRM analysis of both target regions provided successful discrimination that correlated with rep-PCR fingerprinting analysis. Consequently, the HRM analysis has the potential for use in the rapid and accurate classification and typing of yeast species isolated from different foods to determine their sources and routes as well as to prevent contamination.
Effect of Maternal Depression on Brain-derived Neurotrophic Factor Levels in Fetal Cord Blood
Erdem Onder Sonmez,Faruk Uguz,Mine Sahingoz,Gulsum Sonmez,Nazmiye Kaya,Mehmet Akif Camkurt,Zeynel Gokmen,Mustafa Basaran,Kazim Gezginc,Sami Sait Erdem,Hasan Haluk Dulger,Erkan Tasyurek 대한정신약물학회 2019 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.17 No.2
Objective: We aimed to assess the association between cord blood brain-derived neurotrophic factor (BDNF) concentration and maternal depression during pregnancy. Methods: A total of 48 pregnant women, admitted for elective caesarean section to Department of Obstetrics and Gynecology, The Konya Research and Training Hospital and Konya Necmettin Erbakan University Meram Medical Faculty, were included in this study. The study group included 23 women diagnosed as having depression during pregnancy and the control group included 25 pregnant women who did not experience depression during pregnancy. Results: The groups had similar sociodemographic characteristics. Cord blood BDNF concentration was significantly lower in babies born to mothers with major depression as compared with those in the control group. We didn’t find any correlation between the umbilical cord blood BDNF levels and BDI scores. Conclusion: The results suggest that the existence of major depression in pregnant women may negatively affect fetal circulating BDNF levels.