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Influence of Apical Meristem Culture and Thermotherapy on Production of Healthy Fig Plants
Amir Sahraroo,Mesbah Babalar,Ali Ebadi,Mina Kouhi Habibi,Abdollah Khadivi-Khub 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
In this study, production of healthy fig plant using apical meristem culture and thermotherapy in fig (Ficus carica L. cv. Jaami-e-Kan) was conducted. At first, apical meristem (0.2-0.4 and 0.5-0.7 ㎜ in size) of the plant with evident fig mosaic disease (FMDs) symptoms were developed on MS solid medium supplemented with 0.5 ㎎ㆍℓ<SUP>-1</SUP> 6-benzyladenine (BA) and 0.1 ㎎ㆍℓ<SUP>-1</SUP> α-naphthalene acetic acid (NAA). In addition, nodal segment from meristemic microshoots were proliferated on MS medium supplemented with BA (1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>) and NAA (0, 0.1 and 0.5 ㎎ㆍℓ<SUP>-1</SUP>). Finally, the plantlets were rooted on half strength MS medium containing IBA (0.5, 1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>). Significant differences were observed between the treatments. Smaller meristems (0.2-0.4 ㎜) showed the best result in removing the FMDs symptoms after 12 months (93%). Shoot proliferation rate was found to be higher in the medium containing 2 ㎎ㆍℓ<SUP>-1</SUP> BA than other concentrations. Highest numbers of rooted plantlets were obtained from 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP> IBA. At the second experiment, potted plantlets with evident symptoms were subjected with two alternating high temperature regime (35/40℃ and 30/35℃ dark/light) and were evaluated after 4 and 6 weeks. Thermotherapy regime with 35/40℃ dark/light during 6 weeks was significantly (p<0.05) effective on removing the FMDs symptoms (98%).