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- 저자 : Menossi, Marcelo (검색결과 2 건)
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Camila Menossi Sueza Lima,Camila Zanetti Segatto,Gustavo Ricci Zanelli,Gabriel Montoro Nicácio,Renata Navarro Cassu 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.4
Background: Pharmacopuncture is an acupuncture-related technique that has been used to amplify the therapeutic effects of different medications. Objectives: To investigate the analgesic efficacy of a lidocaine injection at acupoints in cats undergoing ovariohysterectomy. Methods: Thirty cats were randomly distributed into two groups (n = 15, per group). The experimental group received a bilateral administration of lidocaine at the following acupoints: Stomach 36 (ST-36) and Spleen 6 (SP-6) (Lido group). The control group did not receive lidocaine (Control group). All cats were sedated with dexmedetomidine and anesthesia was induced with propofol and maintained with isoflurane. Intraoperatively, fentanyl was given to control cardiovascular responses to surgical stimulation. Postoperative pain was assessed at various time points, up to 24 hours after extubation, using the UNESP-Botucatu multidimensional composite pain scale (MCPS) and Glasgow feline composite measure pain scale (CMPS-Feline). Sedation scores were measured at the same time points. Morphine/meloxicam was administered as rescue analgesia. Data were analyzed using t-tests, Fisher´s exact test, the Mann-Whitney test, and the Friedman test (p < 0.05). Results: Intraoperatively, more cats in the Control group required analgesic supplementation than those in the Lido group, but the difference was not significant (p = 0.65). Postoperative pain, sedation scores, and analgesic requirements did not differ between groups. Rescue analgesia was given to 67% (10/15) of the cats in each group. Conclusion: The administration of lidocaine at ST-36 and SP-6 acupuncture points did not provide significant perioperative analgesic benefits in healthy cats undergoing ovariohysterectomy.
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The FBH family of bHLH transcription factors controls ACC synthase expression in sugarcane
Alessio, Valter Miotto,Cavaç,ana, Natale,Dantas, Luí,za Lane de Barros,Lee, Nayoung,Hotta, Carlos Takeshi,Imaizumi, Takato,Menossi, Marcelo Oxford University Press 2018 Journal of experimental botany Vol.69 No.10
<▼1><P>Identification of transcription factors that control the expression of the sugarcane <I>ACS</I> gene, which is likely involved in ethylene-controlled sucrose accumulation and the circadian regulation of ethylene biosynthesis.</P></▼1><▼2><P><B>Abstract</B></P><P>Ethylene is a phytohormone involved in the regulation of several aspects of plant development and in responses to biotic and abiotic stress. The effects of exogenous application of ethylene to sugarcane plants are well characterized as growth inhibition of immature internodes and stimulation of sucrose accumulation. However, the molecular network underlying the control of ethylene biosynthesis in sugarcane remains largely unknown. The chemical reaction catalyzed by 1-aminocyclopropane-1-carboxylic acid synthase (ACS) is an important rate-limiting step that regulates ethylene production in plants. In this work, using a yeast one-hybrid approach, we identified three basic helix-loop-helix (bHLH) transcription factors, homologs of Arabidopsis FBH (FLOWERING BHLH), that bind to the promoter of <I>ScACS2</I> (Sugarcane <I>ACS2</I>), a sugarcane type 3 ACS isozyme gene. Protein–protein interaction assays showed that sugarcane FBH1 (ScFBH1), ScFBH2, and ScFBH3 form homo- and heterodimers in the nucleus. Gene expression analysis revealed that <I>ScFBHs</I> and <I>ScACS2</I> transcripts are more abundant in maturing internodes during afternoon and night. In addition, Arabidopsis functional analysis demonstrated that FBH controls ethylene production by regulating transcript levels of <I>ACS7</I>, a homolog of <I>ScACS2</I>. These results indicate that ScFBHs transcriptionally regulate ethylene biosynthesis in maturing internodes of sugarcane.</P></▼2>
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