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- 저자 : Mengfan Wang (검색결과 4 건)
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Mengfan Wang,Jian Guo,Wei Qi,Rongxin Su,Zhimin He 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.3
In this paper, an effective and green strategy was developed to obtain aglycone isoflavones from soybean through the extraction, hydrolysis, and purification steps. Firstly, a novel ethanol-alkaline extraction method was designed and optimized. The high extraction yield (94.34%)of isoflavones was achieved under the optimal extraction conditions of pH 9.0, 70oC, 60 min, ethanol concentration of 65%, and 1:15 of the solid to liquid ratio. Then, the cellulase (GC-220) was used instead of traditional β-glucosidase to hydrolyze the glycoside isoflavones into aglycone isoflavones which gave an excellent conversion of 95%. Finally, the crude aglycone isoflavones have been purified by an environmental-friendly procedure comprised of ethanol precipitation, ethyl acetate extraction, and water rinse. The final amount of aglycone isoflavones obtained was 1.279 mg for every gram of defatted soybean by this strategy, and the final recovery, and purity of aglycone isoflavones could achieve 80.38 and 87.21%, respectively.
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Yehui Wu,Jing Li,Mengfan Qiao,Dan Meng,Qingling Meng,Jun Qiao,Xingxing Zhang,Lixia Wang,Kuojun Cai,Jinsheng Zhang,Zaichao Zhang,Weiwei Yu,Xuepeng Cai 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6
As an important zoonotic pathogen, Staphylococcus aureus (S. aureus) infection has led to serious mastitis and endometritis in infected dairy cows. HereIn this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine of drug susceptibility and biofilm (BF) formation ability. The eEnterotoxin-related genes were detected, and then the transcription levels of genes related to BF formation were also determined by using reverse transcription-quantitative polymerase chain reactionPCR. Moreover, the pathogenicity of these isolates with different BF formation abilities was determined via by measuring their hemolysis activity, half lethal dose (LD50) and organ bacterial load, respectively. The results showed that 83.4 % of S. aureus isolates could form BF. Among them, 40.8 % of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3 % of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than thoseat of conventional enterotoxin genes. Furthermore, the isolates with a strong BF formation ability had higher LD50 but lower hemolysis ability and organ bacterial load than thoseat of the isolates with weak or no BF ability. However, those isolates without BF ability caused produced more severe pathological changes than thoseat of the isolates with strong BF formation ability. These findings suggested that higher frequencies of BF ability and presence of novel enterotoxin genes were are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolateswhich may posed potential threats to food safety.
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Tumour-Derived Reg3A Educates Dendritic Cells to Promote Pancreatic Cancer Progression
Guo, Jie,Liao, Mengfan,Hu, Xianmin,Wang, Jun Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.9
As a pancreatic inflammatory marker, regenerating islet-derived protein 3A (Reg3A) plays a key role in inflammation-associated pancreatic carcinogenesis by promoting cell proliferation, inhibiting apoptosis, and regulating cancer cell migration and invasion. This study aimed to reveal a novel immuno-regulatory mechanism by which Reg3A modulates tumour-promoting responses during pancreatic cancer (PC) progression. In an in vitro Transwell system that allowed the direct co-culture of human peripheral blood-derived dendritic cells (DCs) and Reg3A-overexpressing/ silenced human PC cells, PC cell-derived Reg3A was found to downregulate CD80, CD83 and CD86 expression on educated DCs, increase DC endocytic function, inhibit DC-induced T lymphocyte proliferation, reduce IL-12p70 production, and enhance IL-23 production by DCs. The positive effect of tumour-derived Reg3A-educated human DCs on PC progression was demonstrated in vivo by intraperitoneally transferring them into PC-implanted severe combined immunodeficiency (SCID) mice reconstituted with human T cells. A Reg3A-JAK2/STAT3 positive feedback loop was identified in DCs educated with Reg3A. In conclusion, as a tumour-derived factor, Reg3A acted to block the differentiation and maturation of the most important antigen-presenting cells, DCs, causing them to limit their potential anti-tumour responses, thus facilitating PC escape and progression.
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Tingting Zhang,Zihui Li,Ya Liu,Kangjia Ding,Yangfeng Guo,Yiyang Xu,Mengfan Sun,Dongfang Wang,Qian Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.12
Polytetrafluoroethylene (PTFE) nano-porous membrane has been widely used in various fields due to its excellent thermal stability and chemical stability. However, PTFE nanofiber membranes with simultaneous hydrophobic and oleophobic properties are essential to promote the application of PTFE. Here, based on inorganic/organic nanohybridization, we report a strategy for constructing nanostructures on fibers by adding nanoparticles, such as MOF and ZrO2. The results of FTIR and XRD confirmed MOF was synthesized successfully. At the same time, the SEM results showed UiO-66-(COOH)2 is spherical with an average diameter of 152 nm, and there is no agglomeration, which is suitable for electrospinning. Further, MOF and ZrO2 were payload into PTFE nanofibers. The results of SEM and AFM confirmed nanostructures will be more uniform and pronounced with the increase of UiO-66-(COOH)2 content, and nanostructures are most obvious when the content of UiO-66-(COOH)2 is 15%. The introduced nanostructures can increase the oil contact angle of the PTFE nano-porous membrane to 110° without introducing other groups, and further improve the water contact angle from 133° to 145°. Meanwhile, the introduction of a certain amount of hydrophilia groups can increase the oil contact angle to more than 120°. The simple strategy is of great significance to expand the application of PTFE fiber membrane in dealing with waste water treatment fields.
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