Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection

Md. Zaherul Islam,윤해근 한국식물생명공학회 2017 JOURNAL OF PLANT BIOTECHNOLOGY Vol.44 No.2

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/ separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobium vitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection

Islam, Md. Zaherul,Yun, Hae Keun The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.26 No.4

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

Characterization of Nine Mlo Family Genes and Analysis of Their Expression Against Pathogen Infections in Vitis flexuosa

Md. Zaherul Islam,Hae Keun Yun 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10

Identification and Expression Profiles of Six Transcripts Encoding Carboxylesterase Protein in Vitis flexuosa Infected with Pathogens

Islam, Md. Zaherul,Yun, Hae Keun The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.4

Plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. Recognition of pathogen attack by the plant resistance gene triggers expression of carboxylesterase genes associated with hypersensitive response. We identified six transcripts of carboxylesterase genes, Vitis flexuosa carboxylesterase 5585 (VfCXE5585), Vf-CXE12827, VfCXE13132, VfCXE17159, VfCXE18231, and VfCXE47674, which showed different expression patterns upon transcriptome analysis of V. flexuosa inoculated with Elsinoe ampelina. The lengths of genes ranged from 1,098 to 1,629 bp, and their encoded proteins consisted of 309 to 335 amino acids. The predicted amino acid sequences showed hydrolase like domains in all six transcripts and contained two conserved motifs, GXSXG of serine hydrolase characteristics and HGGGF related to the carboxylesterase family. The deduced amino acid sequence also contained a potential catalytic triad consisted of serine, aspartic acid and histidine. Of the six transcripts, Vf-CXE12827 showed upregulated expression against E. ampelina at all time points. Three genes (VfCXE5585, VfCXE12827, and VfCXE13132) showed upregulation, while others (VfCXE17159, VfCXE18231, and VfCXE47674) were down regulated in grapevines infected with Botrytis cinerea. All transcripts showed upregulated expression against Rhizobium vitis at early and later time points except VfCXE12827, and were downregulated for up to 48 hours post inoculation (hpi) after upregulation at 1 hpi in response to R. vitis infection. All tested genes showed high and differential expression in response to pathogens, indicating that they all may play a role in defense pathways during pathogen infection in grapevines.

Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa

( Md Zaherul Islam ),( Soon Young Ahn ),( Seon Ae Kim ),( Yong-bum Kwack ),( Hae Keun Yun ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.3

Induced defense responses are regulated through a network of signal transduction pathways. Major signal transduction pathways in plants include salicylic acid (SA)- and jasmonic acid (JA)-mediated signaling pathways. In this study, we attempted to identify potential resistance mechanisms/pathways in Vitis flexuosa and the interaction between resistance and resistance related genes of V. flexuosa and several pathogens. To accomplish this, we investigated transcriptional expression of genes in the SA- and JA-mediated pathway and in the flavonoid biosynthesis pathway in V. flexuosa that had been infected by Elsinoe ampelina, Botrytis cinerea, Colletotrichum acutatum, Erysiphe necator, and Rhizobium vitis using real-time polymerase chain reaction (PCR) analysis. Quantitative real-time PCR revealed that R and R-related genes in V. flexuosa induced defense responses through expression of genes in SA-pathway rather than in the JA-pathway, and R-related genes were more closely correlated with lignin and phytoalexin biosynthesis than anthocyanin biosynthesis against different pathogen infections in V. flexuosa. Specifically, genes such as VfRLK586, VfRLK2422, VfRLK5099, VfRLK29610, VfRLK55257, VfRPS5-like4135, VfRPS5-like4832, VfRPS5-like20585, VfRPS5-like55532, VfEDL2, VfEDL3, VfCXE5585, VfCXE12827, and VfCXE13132 showed close correlation with induction of genes in the SA mediated pathway, lignin, phytoalexin, and anthocyanin biosynthesis. Genes including VfRLK2422, VfRLK5099, VfRLK29610, VfRPS5 like55532, VfEDL3, and VfCXE12827 were also correlated with the JA-mediated signaling pathway in induction of defense responses.

Identificationand Expression Profilesof Six Transcripts Encoding Carboxy-lesterase Protein in Vitis flexuosaInfected with Pathogens

Md. Zaherul Islam,윤해근 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.4

Plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. Recognition of pathogen attack by the plant resistance gene triggers expression of carboxylester-ase genes associated with hypersensitive response. We identified six transcripts of carboxylesterase genes, Vitis flexuosacarboxylesterase 5585 (VfCXE5585), Vf-CXE12827, VfCXE13132, VfCXE17159, VfCXE18231, and VfCXE47674, which showed different expres sion patterns upon transcriptome analysis of V. flexuosa inoculated with Elsinoe ampelina. The lengths of genes ranged from 1,098 to 1,629 bp, and their en-coded proteins consisted of 309 to 335 amino acids. The predicted amino acid sequences showed hydrolase like domains in all six transcripts and contained two conserved motifs, GXSXG of serine hydrolase charac-teristics and HGGGF related to the carboxylesterase family. The deduced amino acid sequence also con-tained a potential catalytic triad consisted of serine, aspartic acid and histidine. Of the six transcripts, Vf-CXE12827 showed upregulated expression against E. ampelina at all time points. Three genes (VfCXE5585, VfCXE12827, and VfCXE13132) showed upregula-tion, while others (VfCXE17159, VfCXE18231, and VfCXE47674) were down regulated in grapevines in-fected with Botrytis cinerea. All transcripts showed upregulated expression against Rhizobium vitis at early and later time points except VfCXE12827, and were downregulated for up to 48 hours post inocula-tion (hpi) after upregulation at 1 hpi in response to R. vitis infection. All tested genes showed high and differ-ential expression in response to pathogens, indicating that they all may play a role in defense pathways dur-ing pathogen infection in grapevines.

Regional Yield Trial with F₁C₇ Clonal Potato Hybrids

Hae Keun Yun,Md. Zaherul Islam,Md. Shafiqul Islam,Md. Eakramul Haque,Md. Abdul Mannaf 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10