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RISS 인기검색어
- 검색키워드
- 저자 : Matousek, J. (검색결과 4 건)
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New Antisense RNA Systems Targeted Against Plant Pathogens
Matousek, J.,Vrba, L.,Kuchar, M.,Pavingerova, D.,Orctova, L.,Ptacek, J.,Schubert, J.,Steger, G.,Beier, H.,Riesner, D. The Korean Society of Plant Biotechnology 2000 식물생명공학회지 Vol.27 No.5
tRNA and 7SL RNA based antisense vehicles were prepared by inserting conserved anti-viral and anti-viroid domains. Anti-PVS coat protein leader sequence (ACPL) and antistructural antihairpin domain of PSTVd (AHII) were inserted in tRNA cassette; anti- zing finger domain of PVS, AHII and anti hop latent viroid ribozyme were inserted in 7SL RNA gene isolated from A. thaliana. These constructs were shown to be transcribed both, in in vitro and in in vivo conditions. However, it followed from our work that closely linked position of PoIII reference genes and PoIIII antisense genes within T-DNA lead to the impairment of RNA expression in transgenic plants. To assay in vivo transcription of antisense genes, hairy root potato cultures were established using h. tumefaciens A4-24 bearing both, Ri plasmid and PoIII-promoterless plant expression vectors with antisense RNA genes. Expression of antisense RNA in transgenic potato tissues was proven by specific RT-PCR reactions.
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Carcass Traits and Meat Quality of Prestice Black-Pied Pig Breed
Matousek, Vaclav,Kernerova, Nadezda,Hysplerova, Klara,Jirotkova, Dana,Brzakova, Michaela Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8
The objective of the study was to evaluate fattening performance, carcass value and meat quality in pigs of Prestice Black-Pied breed in relation to slaughter weight (SW) and gender (barrows and gilts, resp.). Pigs were divided into weight categories: SW1 (75 to 99.9 kg), SW2 (100 to 109.9 kg) and SW3 (110 to 130 kg) and all individual traits were analyzed by the general linear model procedure (SAS 9.3). Average SW of each weight group was as follows: SW1 94.2 kg, SW2 105.8 kg, and SW3 115.2 kg. Differences among average backfat thickness of 36.07 mm in SW1, 40.16 mm in SW2, and 43.21 mm in SW3 were significant (p<0.01). Lean meat content was 48.94% (SW1), 48.78% (SW2), and 48.76% (SW3). Pigs were slaughtered at average weight of 105.7 kg for barrows and 104.4 kg for gilts. Average backfat thickness for barrows was 40.90 mm and 38.72 mm for gilts (significant difference p<0.05). Lean meat content was 48.75% in barrows and 48.91% in gilts. The values of pH45, characterizing the meat of very good quality. The loin in SW3 was darker than the muscles of SW1 and SW2. Drip loss was the lowest in SW1 (1.96%), compared to the highest drip loss in SW3 (2.59%). Content of intramuscular fat was 2.68% in SW3, 2.79% in SW2, and SW1 had the lowest content 2.47%. The values of pH45, colour lightness and drip loss were similar in both genders. However barrows had higher intramuscular fat content by 0.31% than gilts (p<0.05).
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Reachability by paths of bounded curvature in a convex polygon
Ahn, H.K.,Cheong, O.,Matousek, J.,Vigneron, A. Elsevier 2012 Computational Geometry Vol.45 No.1
Let B be a point robot moving in the plane, whose path is constrained to forward motions with curvature at most 1, and let P be a convex polygon with n vertices. Given a starting configuration (a location and a direction of travel) for B inside P, we characterize the region of all points of P that can be reached by B, and show that it has complexity O(n). We give an O(n<SUP>2</SUP>) time algorithm to compute this region. We show that a point is reachable only if it can be reached by a path of type CCSCS, where C denotes a unit circle arc and S denotes a line segment.
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K. Mouralova,L. Benes,J. Bednar,R. Zahradnicek,T. Prokes,R. Matousek,P. Hrabec,Z. Fiserova,J. Otoupalik 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.5
Wire electrical discharge machining (WEDM) is an unconventional machining method for manufacturing complex-shaped, precise components. Considering the high-energy intensity of the wire erosion process, its effective implementation requires a maximum cutting speed while still ensuring the best possible quality of the machined surface. For this latter purpose, we conducted a design of experiment (DoE) comprising 33 cycles and five input factors, or machine setting parameters: Gap voltage, pulse on time, pulse off time, discharge current, and wire feed. In this context, we statistically evaluated the cutting speed in Hadfield steel, as related to machine setting parameters, and also analyzed the machined surfaces of the applied samples. The actual experiment involved examining in detail the topography of the surfaces, using one contact and three non-contact profilers. The surface morphologies were observed via electron microscopy, and a lamella was prepared to facilitate the chemical composition analysis (EDX) with a transmission electron microscope (TEM). To study the subsurface layer and its defects, we produced metallographic specimens of all the samples; the items were observed by means of light and electron microscopes. With a maximum cutting speed the highest quality of the machined surface was achieved; however, the burned cavities can affect the life expectancy of the machined parts. The TEM lamella revealed that the individual alloying elements had segregated into regions, exhibiting high volumes of both manganese and the recast layer and, due to the use of WEDM, a manganese separation layer had formed at the boundary of the recast layer and the base material.
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