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Luo Jinlong,Yang Hua,Peng Xuefeng,Zhang Faqiang,Shu Shilong,Lan Ke,Tu Shengjin,Lu Kai,Cha Xiaoying 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5
Background: Colorectal cancer (CRC) is a prevalent malignant tumor with poor prognosis. Circular RNAs (circRNAs) are key regulators in the progression of CRC. Our study aimed to disclose the role of circ_0000554 in CRC. Methods: The expression of circ_0000554, miR-1205 and LIM and SH3 protein 1 (LASP1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, invasion and migration were monitored using cell counting kit-8 (CCK-8) assay, EdU assay, transwell assay and wound healing assay respectively. The protein levels of C-myc, matrix metallopeptidase 2 (MMP-2) and LASP1 were detected by western blot. Tumor formation assay in nude mice was conducted to explore the role of circ_0000554 in vivo. The association between miR-1205 and circ_0000554 or LASP1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results: circ_0000554 was upregulated in CRC tissues and cells, high circ_0000554 expression was significantly linked to shorter overall survival. Downregulation of circ_0000554 restrained cell growth and metastasis while promoted apoptosis in vitro, and suppressed tumorigenesis of CRC in vivo. Furthermore, mechanism study and rescue experiments confirmed miR-1205 could be sponged by circ_0000554 and its inhibitor reversed the inhibitory effect of circ_0000554 silencing on CRC progression. LASP1 was a target gene of miR-1205 and the upregulation of LASP1 overturned miR-1205-induced effects on CRC cells. Circ_0000554 could elevate LASP1 expression via interacting with miR-1205.
Ze Luo,Jinlong Li,Guozhe Sui,Yan Zhuang,Dongxuan Guo,Rongping Xu,Shuang Liang,Hong Yao,Chao Wang,Shijie Chen 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.8
Developing an efficient, environmentally friendly, and pollution-free catalyst with excellent visible light catalyticactivity is a promising strategy for dye wastewater treatment. Herein, the rod-like hollow BiOClxBr1x (x=1, 0.75, 0.5,0.25, 0), with an adjustable band gap, was successfully prepared using Bi-based metal-organic framework as template. The corresponding hollow assembly and introduction of Br imparted valuable structural advantages and intrinsic characteristicsfor improved photocatalytic activity. Significantly, the degradation efficiency of BiOCl0.5Br0.5 for the RhodamineB (RhB) solution reached 92% under visible light illumination for 90 min, which is considerably higher than that ofCAU-17-derived Bi2O3 and BiOCl. Overall, these findings shed fundamental insight on constructing novel photocatalystswith excellent visible light driven photocatalytic activity and offered a new method for treating dye wastewater.
Characterization of genes coding for galacturonosyltransferaselike (GATL) proteins in rice
Shaobo Li,Jinlong Liu,Mansi Luo,Xin Yan,Chao Yu 한국유전학회 2016 Genes & Genomics Vol.38 No.10
In the present study, seven galacturonosyltransferase- like (GATL) genes (OsGATLs) in rice (Oryza sativa L.) were genome-widely identified and the chromosomal locations and the gene structures of which were characterized. Under normal condition, OsGATL2 and OsGATL3 are highly expressed in root, while OsGATL4 is highly expressed in stem and leaf. Many cis-elements related to stress response and plant hormone were found in the promoter sequence of each OsGATL. The expression patterns of these OsGATL genes under treatment with abscisic acid (ABA), drought and low temperature were assessed by qRT-PCR. The expression levels of most OsGATLs significantly increased following the treatments with drought or low temperature. In addition, physicochemical properties of OsGATLs and phylogenetic analysis with GATL from rice and several other species were performed. 3D structures and protein–protein interaction (PPI) network of OsGATLs were further predicted by Swiss-model and STRING 9.0 database, respectively. The identification and bioinformatic analysis of GATL family in rice could provide reference data for further study on their biological functions, especially in the responsiveness to hormones and stress signaling.
A Novel Active Current Disturbance Method Based on Discrete Wavelet Analysis
Wu Tiezhou,Xiong Jinlong,Wu Xiaomin,Luo Meng 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.12
Islanding detection method of active current disturbances in three-phase photovoltaic-to-grid system makes the common connection point (PCC) voltage appear high frequency components on account of load variation, the surge current occurrence and other factors. This high frequency component cause islands misjudgment. Using Db10 wavelet to detect and analysis PCC’s voltage high-frequency components on real-time based on the active current disturbances principle. And selecting an effective wavelet domain values as the voltage harmonic detection amount islanding detection. Islands simulation test is conducted in the case of the inverter output power and load input power to match, the test results show that this method can quickly detect islanding, and effectively prevent pseudo-island phenomenon of false positives.
Guozhe Sui,Yulin Zhang,Jinlong Li,Yan Zhuang,Dongxuan Guo,Ze Luo,Rongping Xu,Shuang Liang,Hong Yao,Chao Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.4
Organic pollutants in dyed wastewater are hazardous to human health and the environment. The photocatalyticdegradation of pollutants is considered a green treatment with significant social and environmental benefits. Inthis study, hollow BiOCl/TiO2 nanotubes with open pores at both ends, prepared by hydrothermal synthesis, weremodified using phosphoric acid to promote photoelectron transfer. The hollow nanotubes offer an increased numberof electroactive sites, which enhances the photoelectric transfer efficiency and reduces the electron-hole recombinationrate. The modification by H3PO4 significantly optimized the photocatalytic activity of the nanotubes. The results showthat the H3PO4 modified hollow BiOCl/TiO2 (molar ratio of BiOCl to TiO2 is 0.8) nanotubes exhibited the most efficientphotocatalytic performance toward rhodamine B (RhB) with a photodegradation efficiency of up to 98.8% undervisible-light illumination. These nanotubes have broad application prospects for the effective removal of organic pollutantsfrom wastewater and can potentially inform future research on photocatalysts.
Hanxing He,Gefei Li,Jiantao Zhang,Jinlong Zhang,Mingyue Luo,Wenkai Hu,Yamin Lin,Ziyu Deng,Zhicheng Liu,Weizhao Chen,Xu Deng 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.5
A genetic approach was proposed to modify the characteristics of Bacillus subtilis strain WB800 (B. subtilis WB800) for selfhealing of concrete cracks. Three genes, namely gerAa which encodes germination receptors activated by L-alanine, tupA which is responsible for the synthesis of teichuronopeptide, and ca which encodes carbonic anhydrase (CA) catalyzing the synthesis of carbonate ion, were separately transformed into WB800. To protect bacterial cells from being squeezed, microspheres were produced with microcrystal cellulose (MCC) before the introduction of bacteria into the specimens. The results showed that the modified B. subtilis expressing GerA achieved 39.9% of germination ratio compared to 17% by the original host cells. With the transformation of tupA, the modified strain demonstrated higher resistance to alkaline environments, tolerating pH as high as 11, while the original strain only tolerated pH 9. The modified strain expressing CA induced more calcium carbonate than the original cells. Energy dispersive spectroscopy (EDS) identified the produced precipitate to be calcite (CaCO3). Moreover, a mathematical model was developed to optimize the influential factors of calcium precipitation process. Finally, based on the above results, an effective self-healing of concrete crack was achieved. This study may provide a promising strategy to improve the efficiency of bacterial self-healing of concrete cracks.
Effect of Conversion to CTLA4Ig on Tacrolimus-Induced Diabetic Rats
Jin, Long,Lim, Sun Woo,Jin, Jian,Luo, Kang,Ko, Eun Jeong,Chung, Byung Ha,Lin, Hong Li,Yang, Chul Woo Wolters Kluwer Health, Inc. All rights reserved. 2018 Transplantation Vol.102 No.4
<P>Conclusions CTLA4Ig was not diabetogenic, and conversion to CTLA4Ig reduced TAC-induced pancreatic islet injury.</P>