The Achievements and Prospects of Forage Breeding in China

Jin-Feng Yun,Lan Yun,De-Fu Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

The main develop stages of forage breeding in China was reviewed. The achievements in forage germplasm collection, preservation and evaluation were briefed. Registration varieties were classified. The improvement of forage breeding methods and techniques were summarized. Finally, make prospects on forage breeding of China.

β-Elemene Induces Apoptosis in Human Renal-cell Carcinoma 786-0 Cells through Inhibition of MAPK/ERK and PI3K/Akt/mTOR Signalling Pathways

Zhan, Yun-Hong,Liu, Jing,Qu, Xiu-Juan,Hou, Ke-Zuo,Wang, Ke-Feng,Liu, Yun-Peng,Wu, Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Background: Renal-cell carcinoma (RCC) is resistant to almost all chemotherapeutics and radiation therapy. ${\beta}$-Elemene, a promising anticancer drug extracted from a traditional Chinese medicine, has been shown to be effective against various tumors. In the present study, anti-tumor effects on RCC cells and the involved mechanisms were investigated. Methods: Human RCC 786-0 cells were treated with different concentrations of ${\beta}$-elemene, and cell viability and apoptosis were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry, respectively. Protein expression was assayed by western blotting. Autophagy was evaluated by transmission electron microscopy. Results: ${\beta}$-Elemene inhibited the viability of 786-0 cells in a dose- and time-dependent manner. The anti-tumor effect was associated with induction of apoptosis. Further study showed that ${\beta}$-elemene inhibited the MAPK/ERK as well as PI3K/Akt/mTOR signalling pathways. Moreover, robust autophagy was observed in cells treated with ${\beta}$-elemene. Combined treatment of ${\beta}$-elemene with autophagy inhibitors 3-methyladenine or chlorochine significantly enhanced the anti-tumor effects. Conclusions: Our data provide first evidence that ${\beta}$-elemene can inhibit the proliferation of RCC 786-0 cells by inducing apoptosis as well as protective autophagy. The anti-tumor effect was associated with the inhibition of MAPK/ERK and PI3K/Akt/mTOR signalling pathway. Inhibition of autophagy might be a useful way to enhance the anti-tumor effect of ${\beta}$-elemene on 786-0 cells.

Traditional medicine for post-viral olfactory dysfunction: A systematic review

Zou, Xiang-yun,Liu, Xue-han,Lu, Chun-li,Jin, Xin-yan,He, Bai-xiang,Liao, Yi-lei,Liu, Ting,Dai, Yi-dan,Qi, Shi-hao,Sheng, Zhu-jun,Yan, Zhan-feng,Yang, Guo-Yan,Stub, Trine,Liu Jian-Ping 한국한의학연구원 2024 Integrative Medicine Research Vol.13 No.2

Background: Post-viral olfactory dysfunction (PVOD) is the common symptoms of long COVID, lacking of effective treatments. Traditional Chinese medicine (TCM) is claimed to be effective in treating olfactory dysfunction, but the evidence has not yet been critically appraised. We conducted a systematic review to evaluate the effectiveness and safety of TCM for PVOD. Methods: We searched eight databases to identified clinical controlled studies about TCM for PVOD. The Cochrane risk of bias tools and GRADE were used to evaluate the quality of evidence. Risk ratio (RR), mean differences (MD), and 95 % confidence interval (CI), were used for effect estimation and RevMan 5.4.1 was used for data analysis. Results: Six randomized controlled trials (RCTs) (545 participants), two non-randomized controlled trials (non-RCTs) (112 participants), and one retrospective cohort study (30 participants) were included. The overall quality of included studies was low. Acupuncture (n = 8) and acupoint injection (n = 3) were the mainly used TCM therapies. Five RCTs showed a better effect in TCM group. Four trials used acupuncture, and three trials used acupoint injection. The results of two non-RCTs and one cohort study were not statistically significant. Two trials reported mild to moderate adverse events (pain and brief syncope caused by acupuncture or acupoint injection). Conclusions: Limited evidence focus on acupuncture and acupoint injection for PVOD and suggests that acupuncture and acupoint injection may be effective in improving PVOD. More well-designed trials should focus on acupuncture to confirm the benefit. Protocol registration: The protocol of this review was registered at PROSPERO: CRD42022366776.

SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.)

Feng, Hui,Wei, Peng,Piao, Zhong-Yun,Liu, Zhi-Yong,Li, Cheng-Yu,Wang, Yu-Gang,Ji, Rui-Qin,Ji, Shu-Juan,Zou, Ting,Choi, Su-Ryun,Lim, Yong-Pyo Springer-Verlag 2009 TAG. Theoretical and applied genetics. Theoretisch Vol.119 No.2

Compound HRAS/PIK3CA Mutations in Chinese Patients with Alveolar Rhabdomyosarcomas

Liu, Chun-Xia,Li, Xiao-Ying,Li, Cheng-Fang,Chen, Yun-Zhao,Cui, Xiao-Bin,Hu, Jian-Ming,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

The rhabdomyosarcoma (RMS) is the most common type of soft tissue tumor in children and adolescents; yet only a few screens for oncogenic mutations have been conducted for RMS. To identify novel mutations and potential therapeutic targets, we conducted a high-throughput Sequenom mass spectrometry-based analysis of 238 known mutations in 19 oncogenes in 17 primary formalin-fixed paraffin-embedded RMS tissue samples and two RMS cell lines. Mutations were detected in 31.6% (6 of 19) of the RMS specimens. Specifically, mutations in the NRAS gene were found in 27.3% (3 of 11) of embryonal RMS cases, while mutations in NRAS, HRAS, and PIK3CA genes were identified in 37.5% (3 of 8) of alveolar RMS (ARMS) cases; moreover, PIK3CA mutations were found in 25% (2 of 8) of ARMS specimens. The results demonstrate that tumor profiling in archival tissue samples is a useful tool for identifying diagnostic markers and potential therapeutic targets and suggests that these HRAS/ PIK3CA mutations play a critical role in the genesis of RMS.

Prefrontal cortex miR-29b-3p plays a key role in the antidepressant-like effect of ketamine in rats

Yun-Qiang Wan,Jian-Guo Feng,Mao Li,Mao-Zhou Wang,Li Liu,Xueru Liu,Xiao-Xia Duan,Chun-Xiang Zhang,Xiao-Bin Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Ketamine has a rapid, obvious, and persistent antidepressant effect, but its underlying molecular mechanisms remain unknown. Recently, microRNAs (miRNAs) have emerged as important modulators of ketamine’s antidepressant effect. We investigated the alteration in miR-29b-3p in the brain of rats subjected to ketamine administration and chronic unpredictable mild stress (CUMS), and a sucrose preference test and forced swimming test were used to evaluate the rats’ depressive-like state. We used recombination adeno-associated virus (rAAV) or lentivirus-expressing miR-29b-3p to observe the change in metabotropic glutamate receptor 4 (GRM4). Cell culture and electrophysiological recordings were used to evaluate the function of miR-29b-3p. Ketamine dramatically increased miR-29b-3p expression in the prefrontal cortex of the normal rats. The dual luciferase reporter test confirmed that GRM4 was the target of miR-29b- 3p. The miR-29b-3p levels were downregulated, while the GRM4 levels were upregulated in the prefrontal cortex of the depressive-like rats. The ketamine treatment increased miR-29b-3p expression and decreased GRM4 expression in the prefrontal cortex of the depressive-like rats and primary neurons. By overexpressing and silencing miR-29b-3p, we further validated that miR-29b-3p could negatively regulate GRM4. The silencing of miR-29b-3p suppressed the Ca2+ influx in the prefrontal cortex neurons. The miR-29b-3p overexpression contributed to cell survival, cytodendrite growth, increases in extracellular glutamate concentration, and cell apoptosis inhibition. The overexpression of miR- 29b-3p by rAAV resulted in a noticeable relief of the depressive behaviors of the CUMS rats and a lower expression of GRM4. The miR-29b-3p/GRM4 pathway acts as a critical mediator of ketamine’s antidepressant effect in depressive-like rats and could be considered a potential therapeutic target for treating major depression disorder.

Optimization of Dead-end Membrane Filtration to Treat Tunneling Wastewater using the Sustainable Flux

Liu, Qi-Feng,Kim, Seung-Hyun,Yun, Jong-Sup,Moon, Seong-Yong,Lee, Weon-Joon M. Dekker 2007 Separation science and technology Vol.42 No.8

<P> The first object of this study is to confirm the feasibility of sustainable flux in a dead-end mode. The second object is to identify the performance of the MF system operating under sustainable flux and simultaneously using the sustainable flux as a control parameter to evaluate the effects of pre-coagulation via comparing the different operating conditions such as various ranges of the coagulant dose, various pH of the raw water, and comparing the effects of online coagulation with conventional coagulation conditions that with sedimentation. According to the experimental results, the feasibility of sustainable flux in dead-end microfiltration (DEMF) was confirmed. In addition, it was observed that the pH control was the most effective method to improve membrane performance compared with other strategies such as pre-coagulation, and sedimentation in case the raw water with preexistent high concentration metal ions. Relative long-term experiments also confirmed the feasibility of the sustainable flux and showed the importance of backwash to improve the membrane performance.</P>

Roles of Signaling Pathways in the Epithelial-Mesenchymal Transition in Cancer

Liu, Xia,Yun, Fen,Shi, Lin,Li, Zhe-Hai,Luo, Nian-Rong,Jia, Yong-Feng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

The epithelial-mesenchymal transition (EMT) is a cellular process though which an epithelial phenotype can be converted into a phenotype of mesenchymal cells. Under physiological conditions EMT is important for embryogenesis, organ development, wound repair and tissue remodeling. However, EMT may also be activated under pathologic conditions, especially in carcinogenesis and metastatic progression. Major signaling pathways involved in EMT include transforming growth factor ${\beta}(TGF-{\beta})$, Wnt, Notch, Hedgehog and other signaling pathways. These pathways are related to several transcription factors, including Twist, Smads and zinc finger proteins snail and slug. These interact with each other to provide crosstalk between the relevant signaling pathways. This review lays emphasis on studying the relationship between EMT and signaling pathways in carcinogenesis and metastatic progression.

Greenly Synthesized Gold-Alginate Nanocomposites Catalyst for Reducing Decoloration of Azo-Dyes

Yun Li,Gang Li,Wei Li,Feng Yang,Huihong Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

A green synthesis of gold nanoparticles (AuNPs) using aqueous solution of sodium alginate (SA) has been demonstrated in this work. The SA plays the roles of both reducing and stabilizing agent. The surface plasmon resonance (SPR) band of UV-Vis spectrum around 532 nm confirmed the formation of AuNPs. The characterization through high resolution-transmission electron microscopic (HRTEM), energy dispersive X-ray (EDX) spectroscopy and X-ray diffraction (XRD) infer the as-synthesized AuNPs which were spherical in shape with a face cubic crystal (FCC) structure. The results obtained from zeta potential and dynamic light scattering (DLS) suggest the good stability and narrow size distribution of the AuNPs. The size-controlled AuNPs were obtained through adjusting the reaction parameters such as the concentration of SA, pH of the reaction mixture, temperature and the time of incubation. The as-synthesized AuNPs–SA conjugates were employed to prepare AuNPs–SA beads easily based on SA high affinity toward divalent metal ions. The resulting AuNPs–SA beads function as an efficient heterogeneous catalyst in reducing decoloration of azo-dye model compounds, acidic orange 7 (AO7) and reactive orange 5 (RO5), in the presence of sodium borohydride. The reaction rate constants, estimated based on the reduction reactions followed pseudo-first-order kinetics, suggest the high catalytic activity and stability of the AuNPs–SA beads.

Correlation Study of Knowledge and Behavior Regarding Breast Care among Female Undergraduate Students in China

Liu, Meng-Xue,Li, Jian,Geng, Yun-Long,Wang, Yan-Chun,Li, Jie,Chen, Yu-Juan,Ali, Gholam,Tarver, Siobhan L.,Wen, Yu-Feng,Sun, Wen-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: This study aimed to understand the relationship between knowledge level and behavior on breast care in Chinese students, so as to provide strategies for improving the health education of breast care and subsequently for aiding in breast cancer prevention. Materials and Methods: A self-designed questionnaire was used to evaluate breast care knowledge level and characterize related behavior. Correlation analysis was conducted for the knowledge level and behavior. The study was carried out using 597 female undergraduate students in medical and non-medical colleges in Wuhu, China. Results: The average score of breast care knowledge was $5.32{\pm}1.68$ ($5.62{\pm}1.68$ and $5.00{\pm}1.68$ for medical and non-medical students, respectively), with a greater score value for sophomores ($5.59{\pm}1.72$) than freshmen ($5.18{\pm}1.65$). The average score of breast care behavior was $2.21{\pm}1.13$, again with a greater value in sophomores ($2.37{\pm}1.15$) than freshmen ($2.21{\pm}1.13$). A significant positive correlation (r=0.231, p<0.01) between knowledge scores and behavior scores was observed. In addition, various factors, including paying attention to breast care information, receiving breast self-examination guidance, TV program and Internet, were found to influence breast care knowledge. Conclusions: In general, female undergraduate students lack of self-awareness of breast care with a low rate of breast self-examination. It is necessary to carry out health education to improve early detection of breast cancer.