Prognostic Significance of Overexpression of EZH2 and H3k27me3 Proteins in Gastric Cancer

He, Long-Jun,Cai, Mu-Yan,Xu, Guo-Liang,Li, Jian-Jun,Weng, Zi-Jin,Xu, Da-Zhi,Luo, Guang-Yu,Zhu, Sen-Lin,Xie, Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The enhancer of zeste homolog 2 (EZH2) methyl transferase and histone 3 lysine 27 (H3K27me3) protein can repress gene transcription, and their aberrant expression has been observed in various human cancers. This study determined their expression levels in gastric cancer tissues with reference to clinicopathological features and patient survival. We collected 117 gastric cancer and corresponding normal tissues for immunohistochemistry analysis. In gastric cancers, 82/117 (70.1%) were positive for EZH2 and 66/117 (56.4%) for H3K27me3 proteins in contrast to only 5.41% and 7.25% of normal gastric mucosa specimens, respectively. Kaplan-Meier survival data showed the average overall and disease-free survival of EZH2 high expression patients was 25.2 and 20.2 months, respectively, shorter than that with EZH2 low expression (40.5 and 35.9 months). The average overall survival and disease-free survival of high H3K27me3 expression patients was 23.4 and 17.4 months, shorter than without H3K27me3 expression (37.6 and 34.5 months). The average overall survival and disease-free survival of patients with both EZH2 and H3K27me3 expression was 18.8 and 12.9 months, respectively, shorter than that with either alone (34.7 and 31.2 months) or with low levels of both (43.9 and 39.9 months). Multivariate Cox regression analysis showed that H3K27me3 and EZH2 expression, tumor size differentiation and clinical stage were all independent prognostic factors for predicting patient survival. This study demonstrated that detection of both EZH2 and H3K27me3 proteins can predict poor survival of gastric cancer patients, superior to single protein detection. In addition, H3K27me3 and EZH2 protein expression could predict lymph node metastasis.

Formation of heterostructures via direct growth CN on <i>h</i>-BN porous nanosheets for metal-free photocatalysis

He, Zuoli,Kim, Chuhyung,Lin, Lihua,Jeon, Tae Hwa,Lin, Sen,Wang, Xinchen,Choi, Wonyong Elsevier 2017 Nano energy Vol.42 No.-

<P><B>Abstract</B></P> <P>Many recent advances in creating heterostructures based on 2D materials have opened new possibilities in catalysis. This study prepared a new 2D hybrid photocatalyst (CBN-x) consisting of CN and <I>h-</I>BN using low-cost precursors (urea and boric acid). The effects of CN loading on the structure, surface chemistry and photocatalytic activities were systematically investigated. The photocatalytic activities of CBN-x samples were tested for the production of H<SUB>2</SUB> and H<SUB>2</SUB>O<SUB>2</SUB>, which demonstrated markedly enhanced activities without the need of noble metal co-catalysts. However, CBN-x activities for the photocatalytic oxidation of organic compound were not better than that of h-BN. Loading CN on <I>h-</I>BN sheets, C 2p and N 2p orbitals from CN introduce new valence and conduction band edges, which gradually narrowed the bandgap and enhanced light absorption efficiency of the hybrid photocatalysts. In such metal-free systems, electrons generated in CN transfer to h-BN, while photogenerated holes on <I>h-</I>BN transfer to CN, which enhances the charge separation through the heterojunction interface (CN/BN). Therefore, increasing the CN loading enhances the overall efficiency of photocatalysis until excessive loading of CN covers the active sites on h-BN.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this work, we introduced a new kind of 2D heterojunction created by loading CN on h-BN nanosheets. </LI> <LI> The effects of CN loading on the surface chemistry and photocatalytic activities were systematically investigated. </LI> <LI> Photocatalytic H<SUB>2</SUB> and H<SUB>2</SUB>O<SUB>2</SUB> production activities of hybrid CBN-x samples were proven to be enhanced. </LI> <LI> We carried out first-principle calculations which revealed the unique physical features of CN/BN heterostructures. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

EphB1 and Ephrin-B, New Potential Biomarkers for Squamous Cell/adenosquamous Carcinomas and Adenocarcinomas of the Gallbladder

Yuan, Yuan,Yang, Zhu-Lin,Miao, Xiong-Ying,Liu, Zi-Ru,Li, Dai-Qiang,Zou, Qiong,Li, Jing-He,Liang, Lu-Feng,Zeng, Gui-Xiang,Chen, Sen-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Squamous cell/adenosquamous carcinoma (SC/ASC) of the gallbladder are rare tumors and there are few clinical reports in the literature. Herein we report our clinical experience with 46 patients with SC/ASC and 80 with adenocarcinoma (AC). Expression of EphB1 and Ephrin-B in each tumor was determined using immunohistochemical methods for determination of correlations with prognosis. There was no difference in EphB1 and Ephrin-B expression between SC/ASC and AC tumors (P>0.05), but greater expression in those less than 3 cm in diameter, stage I or II (TNM stage), with no lymph node metastases, with no local invasion and treated with radical resection was apparent. Expression of EphB1 (P<0.05) and Ephrin-B (P<0.01) was higher in well differentiated than in poorly differentiated AC tumors. Kaplan-Meier survival analysis indicated that degree of differentiation, tumor diameter, lymph node metastases, local invasion, surgical approach and expression rate of EphB1 and Ephrin-B were closely related to the survival of SC/ASC (P<0.05) and AC patients (P<0.01). Patients with tumors that positive expressed EphB1 and Ephrin-B, whether it is SC/ASC ($P_{SC/ASC}$ =0.000) or AC ($P_{AC}$ =0.000 or $P_{AC}$ =0.002) had longer survival than those negative expression. Cox multivariate analysis indicated a negative correlation between expression of EphB1 or Ephrin-B and overall survival. Hence, EphB1 and Ephrin-B could be regarded as independent good prognostic factorsand important biological markers for SC/ASC and AC of gallbladder.

창조성의 협동력이 중국 유학생의 문화적응 스트레스와 건강증진행위에 미치는 차이

이염삼 ( Li Yan-sen ),이정은 ( Lee Jung-eun ),하전침장 ( He Dian Chen Zhang ),강곡영 ( Qlang Gu-ylng ),양우림 ( Yang Yu-lin ),조현철 ( Cho Hyun-choul ),백우석 ( Baek Woo-suk ) 창조교육학회 2019 창조교육논총 Vol.21 No.-

This research takes Chinese overseas students of W university in Jeollabuk-do in 2018 as subjects, and attempts to understand the influence of acculturation stress on health behaviors. Firstly, the average acculturation stress of Chinese overseas students is 1.88±.40, and there is no significant stress. For general characteristics, differences (p<.01) occurred in TOPIK, underlying factor “designated factor” (p<.01)“fear”, “perceived hostility”, benefitted from scholarship in“others” is “cultural shock”(p<.05), differences of “designated hostility”(p<.001), “cultural shock”, “sense of guilt” (p<.0-05) under healthy condition. Secondly, the average of Chinese overseas students’health-enhancing behaviors is 3.6±.5 (5 as full scores), which is a high score in general. For general characteristics, based on“health risk”(p<.05), a series of differences“ physical activities”(p<.01),“nutrition”(pm.001), a significant difference occurred in“pressure management”from (p<.05). Difference occurred in “physical activity” (p<.05) of living status, and there is a significant difference in“physical activity”(p<.01) and “stress management”(p<.001) in health condition. Based on above research findings, all Chinese overseas students live in Jeolla buk-do should improve health responsibility awareness in terms of health-enhancing behaviors, and special health management is needed for physical activity and cultural adaptation conditions.

Microscopic Mechanisms and Acoustic Emission Characteristics of Sandy Mudstone under Different Water Saturations

Xiao-ming Sun,Fu-kun Shi,Ming-qun Zhu,Jia-xu Ding,Lin-sen He,Zhi-hu Li,Tong Zhang,Cheng-yu Miao 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

In order to study the damage mechanism of surrounding rocks under water-rock coupling, an experimental study on the microscopic law of water absorption and mechanics of sandy mudstone was carried out in the sandy mudstone stratum of Wanfu coal mine in Shandong, China. The results indicate that the strength of sandy mudstone exhibits three stages of relative softening, rapid softening, and decelerated softening. The speed of water molecules entering the microscopic pore structure was analyzed based on the percentage of water molecules in the pore structure. The acoustic emission energy release process is divided into energy accumulation stage, stable release stage and intense release stage. With the increase of water content saturation,  (Spectral width of the multifractal spectrum) tends to decrease and the distribution of events is gradually uniform. The action of water gradually expands and penetrates the cracks and pores inside the sandy mudstone, which makes f (Frequency of occurrence of the signal) change from negative to positive values.