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- 저자 : Lenore, Lyons (검색결과 6 건)
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DNA 및 RNA 의 특수한 순서를 검지 할 수 있는 새로운 표식방법
Lenore Gardner 생화학분자생물학회 1983 생화학분자생물학회 소식 Vol.3 No.4
이 논문은 동위원소를 쓰지 않고 DNA probe를 labeling하는 방법에 관한 것이다. Biotinylate된 deoxyuridine triphosphate를 nick translation에 의하여 DNA probe에 끼운다. 이렇게 만든 probe의 녹는점은 약 5°C 정도 낮아지지만, 이 Biotinylate된 DNA probe의 hybridization kinetics는 p-label된 DNA probe의 그것과 비슷하다. 이 Probe의 여러 가지 사용법이 여기서 논의되고 있고 in situ hybridization이나 nitrocellulose membrane에 hybridize된 것을 볼 수 있는 두 가지 방법도 (??)되어 있다. Detek Ⅰ-f는 첫 단계에서 goat anti-biotin 항체를 사용하고 그 다음에 FITC rabbit anti-goat conjugate를 사용하고 있다. 그러므로 이 hybridize된 것은 일반적인 형광 현미경으로 그 위치를 알 수 있다. Detek Ⅰ- hrp는 streptavidinhorseradish peroxidase complex를 사용한다. 그러므로 hybridize 된 것은 표준 peroxidase assay 에 의하여 검출할 수 있다. viral DNA, Viral mRNA, cellular DNA(highly-re-peated mouse satellite DNA)의 위치를 알기 위하여 Biopro be를 사용한 예가 제시되어 있다.
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The effect of three Korean traditional medicines on the growth rate of cultured human keratinocytes
Chung, Seok Hee,Hiroto Terashi,Lenore M. Rhodes,Moon, Namdoo,William R. Dunham,Cynthia L. Marcelo 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-
The effect of three different Korean Traditional Medicines (KTM) was studied on several functional parameters of adult human cells in culture. The cells were non-transformed strains of normal, skin epidermal cells (keratinocytes) from adult humans. Aqueous extracts of the herbal medicines were tested using two types of cell strains: one type was essential fatty acid deficient (EFAD) cells which grow rapidly in medium that was low in calcium and had no essential fatty acids; the second type was a cell strain grown in medium supplemented with essential fatty acid (EFA-supplemented). These cells had much slower, in vivo skin growth rates, and the fatty acid composition resembled that measured in epidermal biopsy tissue. The KTMs chosen for this study were tae-gang-hual-tang (for treating osteoarthritis), hual-ak-tang (for pain relief) and sip-zeon-tae-bo-tang (for fortifying immune systems). Because high proliferation rates usually correlate with skin inflammation and because many of the chemotactic agents mediating inflammatory response are modified fatty acids, this study focused on cell growth rate and membrane fatty acid composition as signals for the effects of the herbal medicines. By monitoring growth rate, these experiments measured both a stimulatory and a regulatory effect on the growth of keratinocytes. Some toxicity was seen at the highest doses of the KTMs. These effects were modeled mathematically, and the results showed varying effects on growth rate depending on dose and herbal recipe. The fitting parameters were discussed as they relate to biological function. The experimental design was also discussed and alternatives were suggested. ⓒ 2001 Elsevier Science lreland Ltd. All rights reserved.
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Automatic Surface Matching for the Registration of LIDAR Data and MR Imagery
Ayman Fawzy Habib,Rita Wai Ting Cheng,Eui-Myoung Kim,Edson Aparecido Mitishita,Richard Frayne,Janet Lenore Ronsky 한국전자통신연구원 2006 ETRI Journal Vol.28 No.2
Several photogrammetric and geographic information system applications such as surface matching, object recognition, city modeling, environmental monitoring, and change detection deal with multiple versions of the same surface that have been derived from different sources and/or at different times. Surface registration is a necessary procedure prior to the manipulation of these 3D datasets. This need is also applicable in the field of medical imaging, where imaging modalities such as magnetic resonance imaging (MRI) can provide temporal 3D imagery for monitoring disease progression. This paper will present a general automated surface registration procedure that can establish correspondences between conjugate surface elements. Experimental results using light detection and ranging (LIDAR) and MRI data will verify the feasibility, robustness, and accuracy of this approach.
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Association of brain heptachlor epoxide and other organochlorine compounds with lewy pathology
Ross, G. Webster,Abbott, Robert D.,Petrovitch, Helen,Duda, John E.,Tanner, Caroline M.,Zarow, Chris,Uyehara‐,Lock, Jane H.,Masaki, Kamal H.,Launer, Lenore J.,Studabaker, William B.,White, Lon R. John Wiley Sons, Inc. 2019 Movement disorders Vol.34 No.2
<P><B>ABSTRACT</B></P><P><B>Background</B></P><P>Organochlorine pesticides are associated with an increased risk of Parkinson's disease. A preliminary analysis from the Honolulu‐Asia Aging Study suggested that heptachlor epoxide, a metabolite from an organochlorine pesticide extensively used in Hawaii, may be especially important. This was a cross sectional analysis to evaluate the association of heptachlor epoxide and other organochlorine compounds with Lewy pathology in an expanded survey of brain organochlorine residues from the longitudinal Honolulu‐Asia Aging Study.</P><P><B>Methods</B></P><P>Organochlorines were measured in frozen occipital or temporal lobes in 705 brains using gas chromatography with mass spectrometry. Lewy pathology was identified using hematoxylin and eosin‐ and α‐synuclein immunochemistry‐stained sections from multiple brain regions.</P><P><B>Results</B></P><P>The prevalence of Lewy pathology was nearly doubled in the presence versus the absence of heptachlor epoxide (30.1% versus 16.3%, <I>P</I> < 0.001). Although associations with other compounds were weaker, hexachlorobenzene (<I>P</I> = 0.003) and α‐chlordane (<I>P</I> = 0.007) were also related to Lewy pathology. Most of the latter associations, however, were a result of confounding from heptachlor epoxide. Neither compound was significantly related to Lewy pathology after adjustment for heptachlor epoxide. In contrast, the association of heptachlor epoxide with Lewy pathology remained significant after adjustments for hexachlorobenzene (<I>P</I> = 0.013) or α‐chlordane (<I>P</I> = 0.005). Findings were unchanged after removal of cases of PD and adjustment for age and other characteristics.</P><P><B>Conclusions</B></P><P>Organochlorine pesticides are associated with the presence of Lewy pathology in the brain, even after exclusion of PD cases. Although most of the association is through heptachlor epoxide, the role of other organochlorine compounds is in need of clarification. © 2018 International Parkinson and Movement Disorder Society</P>
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