http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Junsung,Lee, Hyoungjin,Goh, Unbyeol,Kim, Jiyoung,Jeong, Moonkyoung,Lee, Jean,Park, Ji-Ho American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.11
<P>Engineering of extracellular vesicles (EVs) without affecting biological functions remains a challenge, limiting the broad applications of EVs in biomedicine. Here, we report a method to equip EVs with various functional agents, including fluorophores, drugs, lipids, and bio-orthogonal chemicals, in an efficient and controlled manner by engineering parental cells with membrane fusogenic liposomes, while keeping the EVs intact. As a demonstration of how this method can be applied, we prepared EVs containing azide-lipids, and conjugated them with targeting peptides using copper-free click chemistry to enhance targeting efficacy to cancer cells. We believe that this liposome-based cellular engineering method will find utility in studying the biological roles of EVs and delivering therapeutic agents through their innate pathway.</P>
Lee, Seunghwan,Lee, Sanghyeok,Kim, Hyo-Jin,Choi, Sung Min,An, Hyegsoon,Park, Mi Young,Shin, Jisu,Park, Jung Hoon,Ahn, Junsung,Kim, Donghwan,Ji, Ho-Il,Kim, Hyoungchul,Son, Ji-Won,Lee, Jong-Ho,Kim, Byun The Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.31
<P>Solid oxide fuel cell (SOFC) technology offers tremendous potential for highly efficient and clean power generation. However, its commercialization has lagged owing to the lack of long-term stability. Among the various sources of performance degradation, the interdiffusion between the cathode and electrolyte has been identified as a predominant factor. Herein, we demonstrate a highly reliable diffusion-blocking layer that completely suppresses detrimental chemical interactions at elevated temperatures. This diffusion-blocking layer is constructed <I>via</I> a bilayer approach, in which the top and bottom layers perform individual functions to precisely control the bulk and interfacial properties. Harnessing two types of specially designed nanoparticles for each part enables the realization of the desired film structure. Consequently, the formation of insulating phases and decomposition of the cathode are effectively prevented, resulting in a remarkable improvement in performance and stability. The scalability and feasibility of mass production are verified <I>via</I> the fabrication of large cells (10 cm × 10 cm) and a multi-cell stack. The stack in which the bilayer technique is implemented exhibits an extremely low degradation rate of 0.23% kh<SUP>−1</SUP>, which fulfills the strict lifetime requirement for market penetration. This work highlights a scalable, cost-effective, and reproducible method for the production of highly durable multilayer energy devices, including SOFCs.</P>
Lee, Junsung,Kim, Jiyoung,Jeong, Moonkyoung,Lee, Hyoungjin,Goh, Unbyeol,Kim, Hyaeyeong,Kim, Byungji,Park, Ji-Ho American Chemical Society 2015 NANO LETTERS Vol.15 No.5
<P>Natural membrane vesicles (MVs) derived from various types of cells play an essential role in transporting biological materials between cells. Here, we show that exogenous compounds are packaged in the MVs by engineering the parental cells via liposomes, and the MVs mediate autonomous intercellular migration of the compounds through multiple cancer cell layers. Hydrophobic compounds delivered selectively to the plasma membrane of cancer cells using synthetic membrane fusogenic liposomes were efficiently incorporated into the membrane of MVs secreted from the cells and then transferred to neighboring cells via the MVs. This liposome-mediated MV engineering strategy allowed hydrophobic photosensitizers to significantly penetrate both spheroids and in vivo tumors, thereby enhancing the therapeutic efficacy. These results suggest that innate biological transport systems can be in situ engineered via synthetic liposomes to guide the penetration of chemotherapeutics across challenging tissue barriers in solid tumors.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-5/nl5047494/production/images/medium/nl-2014-047494_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5047494'>ACS Electronic Supporting Info</A></P>
Lee, Changkeun,Hong, Seongjin,Noh, Junsung,Lee, Junghyun,Yoon, Seo Joon,Kim, Taewoo,Kim, Hosang,Kwon, Bong-Oh,Lee, Hanbyul,Ha, Sung Yong,Ryu, Jongseong,Kim, Jae-Jin,Kwon, Kae Kyoung,Yim, Un Hyuk,Khim, Elsevier Applied Science Publishers 2019 Environmental pollution Vol.252 No.1
<P><B>Abstract</B></P> <P>While various bioremediation techniques have been widely used at oil spill sites, the in situ efficiency of such techniques on recovering the benthic communities in intertidal areas has not been quantified. Here, the performance of several bioremediation tools such as emulsifiers, multi-enzyme liquid (MEL), microbes, and rice-straw was evaluated by a 90-days semi-field experiment, particularly targeting recovery of benthic community. Temporal efficiency in the removal of sedimentary total petroleum hydrocarbons (TPH), reduction of residual toxicity, and recovery of bacterial diversity, microalgal growth, and benthic production was comprehensively determined. Concentrations of TPH and amphipod mortality for all treatments rapidly decreased within the first 10 days. In addition, the density of bacteria and microphytobenthos generally increased over time for all treatments, indicating recovery in the benthic community health. However, the recovery of some nitrifying bacteria, such as the class Nitrospinia (which are sensitive to oil components) remained incomplete (13–56%) during 90 days. Combination of microbe treatments showed rapid and effective for recovering the benthic community, but after 90 days, all treatments showed high recovery efficiency. Of consideration, the “no action” treatment showed a similar level of recovery to those of microbe and MEL treatments, indicating that the natural recovery process could prevail in certain situations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Residual hydrocarbons and amphipod toxicity rapidly decreased within 10 days. </LI> <LI> Quick recovery found for most of the bacteria, but some remained incomplete at >90 d. </LI> <LI> Microphytobenthos community took more than 90 days to recover against oil exposure. </LI> <LI> Combination of microbe treatments were effective for recovering the benthic community. </LI> <LI> “No action” showed comparable recovery to others, indicating prevailing natural recovery. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Changkeun,Kwon, Bong-Oh,Hong, Seongjin,Noh, Junsung,Lee, Junghyun,Ryu, Jongseong,Kang, Seong-Gil,Khim, Jong Seong Elsevier 2018 Environmental pollution Vol.241 No.-
<P><B>Abstract</B></P> <P>The potential leakage from marine CO<SUB>2</SUB> storage sites is of increasing concern, but few studies have evaluated the probable adverse effects on marine organisms. Fish, one of the top predators in marine environments, should be an essential representative species used for water column toxicity testing in response to waterborne CO<SUB>2</SUB> exposure. In the present study, we conducted fish life cycle toxicity tests to fully elucidate CO<SUB>2</SUB> toxicity mechanism effects. We tested sub-lethal and lethal toxicities of elevated CO<SUB>2</SUB> concentrations on marine medaka (<I>Oryzias melastigma</I>) at different developmental stages. At each developmental stage, the test species was exposed to varying concentrations of gaseous CO<SUB>2</SUB> (control air, 5%, 10%, 20%, and 30%), with 96 h of exposure at 0–4 d (early stage), 4–8 d (middle stage), and 8–12 d (late stage). Sub-lethal and lethal effects, including early developmental delays, cardiac edema, tail abnormalities, abnormal pigmentation, and mortality were monitored daily during the 14 d exposure period. At the embryonic stage, significant sub-lethal and lethal effects were observed at pH < 6.30. Hypercapnia can cause long-term and/or delayed developmental embryonic problems, even after transfer back to clean seawater. At fish juvenile and adult stages, significant mortality was observed at pH < 5.70, indicating elevated CO<SUB>2</SUB> exposure might cause various adverse effects, even during short-term exposure periods. It should be noted the early embryonic stage was found more sensitive to CO<SUB>2</SUB> exposure than other developmental stages of the fish life cycle. Overall, the present study provided baseline information for potential adverse effects of high CO<SUB>2</SUB> concentration exposure on fish developmental processes at different life cycle stages in marine ecosystems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Adverse effects of elevated CO<SUB>2</SUB> varied cross developmental stages of marine medaka. </LI> <LI> Embryo developmental delay to elevated CO<SUB>2</SUB> showed in concentration-dependent manner. </LI> <LI> Middle stage of development was the most sensitive period to CO<SUB>2</SUB> exposure for mortality. </LI> <LI> Despite transfer to clean water, CO<SUB>2</SUB> exposed embryos could not be recovered. </LI> <LI> Key symptoms by elevated CO<SUB>2</SUB> exposure encompassed mortality and cardiac edema. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Junsung,Goh, Unbyeol,Lee, Hyoung-Jin,Kim, Jiyoung,Jeong, Moonkyoung,Park, Ji-Ho American Chemical Society 2017 MOLECULAR PHARMACEUTICS Vol.14 No.2
<P>Efficient delivery of drugs to the retina is critical but difficult to achieve with current methods. There have been a number of attempts to use intravitreal injection of liposomes, artificial vesicles composed of a phospholipid bilayer, to overcome the limitations of conventional intravitreal injection (short retention time, toxicity, poor penetration, etc.). Here, we report an optimal liposomal formulation that can diffuse through the vitreous humor, deliver the incorporated agents to all retinal layers effectively, and maintain them for a relatively long time. We first delivered lipophilic compounds and phospholipid-conjugated hydrophilic agents to the inner limiting membrane using engineered liposomes. Subsequently, the agents penetrated the retina deeply, presumably via extracellular vesicles, nanoscale vesicles secreted from retinal-associated cells. These results suggest that this engineered liposomal formulation can leverage the biological transport system for effective retinal penetration of lipophilic and lipid-conjugated agents.</P>
Meta-analysis of factors affecting milk component yields in dairy cattle
Lee, Junsung,Seo, Jakyeom,Lee, Se Young,Ki, Kwang Seok,Seo, Seongwon Korean Society of Animal Sciences and Technology 2014 한국축산학회지 Vol.56 No.2
The objectives of this study were thus to identify most significant factors that determine milk component yield (MCY) using a meta-analysis and, if possible, to develop equations to predict MCY using variables that can be easily measured in the field. A literature database was constructed based on the research articles published in the Journal of Dairy Science from Oct., 2007 till May, 2010. The database consisted of a total of 442 observed means for MCY from 118 studies. The candidate factors that determine MCY were those which can be routinely measured in the field (e.g. DMI, BW, dietary forage content, chemical composition of diets). Using a simple linear regression, the best equations for predicting milk fat yield(MFY) and milk protein yield (MPY) were $MFY=0.351({\pm}0.068)+0.038({\pm}0.003)$ DMI ($R^2=0.27$), and $MPY=0.552({\pm}0.071)+0.031({\pm}0.002)DMI-0.004({\pm}0.001)$ FpDM (%, forage as a percentage of dietary DM) ($R^2=0.38$), respectively. The best equation for predicting milk fat content (%) explained only 12% of variations in milk fat content, and none of a single variable can explain more than 5% of variations in milk protein content. We concluded that among the tested variables, DMI was the only significant factor that affects MFY and both DMI and FpDM significantly affect MPY. However, predictability of linear equations was relatively low. Further studies are needed to identify other variables that can predict milk component yield more accurately.