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Extraction of parishin B and parishin C from Gastrodiae Rhizoma by subcritical water technology
Guozhen Wu,Hongjing Dong,Jia Li,Lanping Guo,Yan Cheng,Yanling Geng,Xiao Wang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-
Subcritical water extraction (SWE) was used to extract bioactive parishin B (PB) and parishin C (PC) fromGastrodiae Rhizoma. Extraction conditions were optimized by Box-Behnken design (BBD) of responsesurface methodology (RSM). The highest yield of PB was 7.46 ± 0.18 mg/g when the solid/liquid ratio(SLR) was 4.5 mg/mL at an extraction temperature of 118 C and extraction time of 50 min, and the highestyield of PC was 5.27 ± 0.07 mg/g when the SLR was 22 mg/mL at an extraction temperature of 145 Cand extraction time of 23 min. Compared with heating reflux extraction the yield of PB was about 1.3times higher, and that of PC about 2.6 times higher; Compared with ultrasound assisted extraction theyield of PB was about 3 times higher, and that of PC about 6 times higher. Degradation of parishin Aby SWE showed that the increasing mechanism of PB and PC mainly attributed to the conversion of PAinto PB and PC. This study suggests that SWE was an efficient and green way to extract natural productswith greater values.
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Chenglong Sun,Shuangshuang Ma,Lili Li,Daijie Wang,Wei Liu,Feng Liu,Lanping Guo,Xiao Wang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6
Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asiancountries. Its root and rhizome have long been used for the treatment of cardiovascular and hematologicaldiseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous planttissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will alsoprovide a highly informative approach to understand the complex molecular changes in the processing ofPanax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize thespatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panaxnotoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine,gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizomeand root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during thesteaming of Panax notoginseng root were also characterized in this study. And, a series of metabolitessuch as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng weresuccessfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panaxnotoginseng metabolic networks, but also provide dire
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