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Van Dam Lai,Jong-Seung Kim,In-Pil Mo 한국가금학회 2020 韓國家禽學會誌 Vol.47 No.2
Fowl cholera is an infectious disease caused by Pasteurella multocida that contributes to high economic loss in the commercial chicken industry. Three Pasteurella multocida strains were isolated from outbreaks of acute fowl cholera in the Korean layer farms from 2018 to 2019. One strain was identified and serotyped using capsular PCR typing. This strain was also genotyped by lipopolysaccharide (LPS) PCR typing as A: L3, whereas other strains were non-typable. The multilocus sequence typing (MLST) result showed that the A: L3 strain is sequence type (ST) 134; the non-typable strains were recorded as the following new STs: ST 366 and ST 374. Using phylogenetic tree analysis based on MLST sequences, we determined that ST 366 and ST 374 are closely related to the reference strains that were previously isolated from duck and chicken in Korea, and they were highly prevalent within the Korean cluster. In conclusion, Pasteurella multocida strains were identified and isolated in this study. Furthermore, this is the first report of using MLST to determine the prevalence of fowl cholera in Korea.
국내 산란계의 주요 전염성 질병에 대한 혈청학적 모니터링
민봉철,Lai Van Dam,김강산,김태식,손주성,모인필 한국가금학회 2019 韓國家禽學會誌 Vol.46 No.4
Serological monitoring has been conducted worldwide for early diagnosis of disease and monitoring of immune status in poultry. This study was conducted to evaluate the immune status of layers with sera submitted to the Avian Disease Laboratory, Chungbuk National University from 2015 to 2017. The test results were analyzed by the time submitted and by the age of the chicks. Low pathogenic avian influenza (LPAI) showed a low positive rate of antibody compared with those of Newcastle disease, indicating that domestic vaccination against LPAI was not sufficient. The antibody profile of infectious bronchitis (IB) depicted high level of titer and a low tendency of CV as compared to the uninfected control flocks, which indicated that most layer farms have been exposed to the field IB virus. In case of avian metapneumovirus infection (aMPV) and Mycoplasma synoviae (MS), since the introduction of the vaccine in 2011 and 2017, respectively, the positive rate and the titer level were higher than those in pevious times. No significant difference in the changes of seasonal result was observed, indicating proper vaccination and improvement in biosecurity and management.
김강산,김태식,손주성,Van Dam Lai,박정은,왕승준,정원화,모인필 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.5
Korea is located within the East Asian-Australian flyway of wild migratory birds duringthe fall and winter seasons. Consequently, the likelihood of introduction of numeroussubtypes and pathotypes of the Avian influenza (AI) virus to Korea has been thought to bevery high. In the current study, we surveyed wild bird feces for the presence of AI virus thathad been introduced to Korea between September 2017 and February 2018. To identify andcharacterize the AI virus, we employed commonly used methods, namely, virus isolation (VI)via egg inoculation, real-time reverse transcription-polymerase chain reaction (rRT-PCR),conventional RT-PCR (cRT-PCR) and a newly developed next generation sequencing (NGS)approach. In this study, 124 out of 11,145 fresh samples of wild migratory birds tested wererRT-PCR positive; only 52.0% of VI positive samples were determined as positive by rRT-PCRfrom fecal supernatant. Fifty AI virus specimens were isolated from fresh fecal samples andtyped. The cRT-PCR subtyping results mostly coincided with the NGS results, although NGSdetected the presence of 11 HA genes and four NA genes that were not detected by cRTPCR. NGS analysis confirmed that 12% of the identified viruses were mixed-subtypes whichwere not detected by cRT-PCR. Prevention of the occurrence of AI virus requires a workflowfor rapid and accurate virus detection and verification. However, conventional methodsof detection have some limitations. Therefore, different methods should be combinedfor optimal surveillance, and further studies are needed in aspect of the introduction andapplication of new methods such as NGS.