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Lade, Harshad,Paul, Diby,Kweon, Ji Hyang Molecular Diversity Preservation International (MD 2014 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.15 No.2
<P>The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like <I>N</I>-acyl homoserine lactones (AHLs). In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, <I>Chromobacterium violaceum</I> CV026 and <I>Agrobacterium tumefaciens</I> A136. A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of <I>Aeromonas</I> and <I>Enterobacter sp</I>. in AHLs production; however few a species of <I>Serratia</I>, <I>Leclercia</I>, <I>Pseudomonas</I>, <I>Klebsiella</I>, <I>Raoultella</I> and <I>Citrobacte</I>r were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL. The occurrence of AHLs in sludge extract and dominance of <I>Aeromonas</I> and <I>Enterobacter sp.</I> in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling.</P>
Effects of fines content on void ratio, compressibility, and static liquefaction of silty sand
Lade, Poul V.,Yamamuro, Jerry A.,Liggio, Carl D. Jr. Techno-Press 2009 Geomechanics & engineering Vol.1 No.1
Many aspects of the behavior of sands are affected by the content of non-plastic fine particles and these various aspects should be included in a constitutive model for the soil behavior. The fines content affects maximum and minimum void ratios, compressibility, shear strength, and static liquefaction under undrained conditions. Twenty-eight undrained triaxial compression tests were performed on mixtures of sand and fine particles with fines contents of 0, 10, 20, 30, 50, 75, and 100% to study the effects of fines on void ratio, compressibility, and the occurrence of static liquefaction. The experiments were performed at low consolidation pressures at which liquefaction may occur in near-surface, natural deposits. The presence of fines creates a particle structure in the soil that is highly compressible, enhancing the potential for liquefaction, and the fines also alter the basic stress-strain and volume change behavior, which should be modeled to predict the occurrence of static liquefaction in the field. The void ratio at which liquefaction occurs for each sand/fines mixture was determined, and the variation of compressibility with void ratio was determined for each mixture. This allowed a relation to be determined between fines content, void ratio, compressibility, and the occurrence of static liquefaction. Such relations may vary from sand to sand, but the present results are believed to indicate the trend in such relations.
Assessment of Rainwater Harvesting Potential in Ibadan, Nigeria
Lade, Omolara,Oloke, David Korean Society of Environmental Engineers 2013 Environmental Engineering Research Vol.17 No.1
Recently Ibadan in southwestern Nigeria has been facing severe water shortage due to the increase of population, social and economic activities. In order to meet the shortfall, attempts to utilize rainwater harvesting (RWH) have been made to provide an alternative source of water supply. A desk study was conducted to review various RWH technologies locally, regionally and globally. A hydrological analysis was also carried out using rainfall data for 30 years from two meteorological stations, with the aim of providing a more sustainable RWH system for water supply to private individuals, organizations, and government agencies. RWH is found to be technically feasible based on the prevailing rainfall pattern with over 90% of households having a rooftop constructed from technically appropriate materials. Results of the study indicate that an average roof of $80m^2$ will collect 82,835 L/yr (45 L/person/day) for a family of five people which is about the required water demand for drinking and cooking purposes. Hence, the capacity of storage tanks and the catchment area required for an all-purpose water supply system based on RWH are quite large. These can be reduced to affordable sizes, by collecting and storing water for cooking and drinking only while non-potable uses are supplemented by water from other sources. However, it must be highlighted that due to the type of roofing material, rainwater should go through proper treatment in order to be used for potable purposes. This study clearly shows that Ibadan city has a good rainwater harvesting potential.
( Harshad Lade ),( Diby Paul ),( Jihyang Kweon ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11
This work investigated the potential of curcumin (CCM) and (-)-epigallocatechin gallate (EGCG) to inhibit N-acyl homoserine lactone (AHL)-mediated biofilm formation in gramnegative bacteria from membrane bioreactor (MBR) activated sludge. The minimum inhibitory concentrations (MICs) of CCM alone against all the tested bacteria were 200-350 μg/ml, whereas those for EGCG were 300-600 μg/ml. Biofilm formation at one-half MICs indicated that CCM and EGCG alone respectively inhibited 52-68% and 59-78% of biofilm formation among all the tested bacteria. However, their combination resulted in 95-99% of biofilm reduction. Quorum sensing inhibition (QSI) assay with known biosensor strains demonstrated that CCM inhibited the expression of C4 and C6 homoserine lactones (HSLs)-mediated phenotypes, whereas EGCG inhibited C4, C6, and C10 HSLs-based phenotypes. The Center for Disease Control biofilm reactor containing a multispecies culture of nine bacteria with onehalf MIC of CCM (150 μg/ml) and EGCG (275 μg/ml) showed 17 and 14 μg/cm2 of extracellular polymeric substances (EPS) on polyvinylidene fluoride membrane surface, whereas their combination (100 μg/ml of each) exhibited much lower EPS content (3 μg/cm2). Confocal laser scanning microscopy observations also illustrated that the combination of compounds tremendously reduced the biofilm thickness. The combined effect of CCM with EGCG clearly reveals for the first time the enhanced inhibition of AHL-mediated biofilm formation in bacteria from activated sludge. Thus, such combined natural QSI approach could be used for the inhibition of membrane biofouling in MBRs treating wastewaters.
Assessment of Rainwater Harvesting Potential in Ibadan, Nigeria
Omolara Lade,David Oloke 대한환경공학회 2013 Environmental Engineering Research Vol.18 No.2
Recently Ibadan in southwestern Nigeria has been facing severe water shortage due to the increase of population, social and economic activities. In order to meet the shortfall, attempts to utilize rainwater harvesting (RWH) have been made to provide an alternative source of water supply. A desk study was conducted to review various RWH technologies locally, regionally and globally. A hydrological analysis was also carried out using rainfall data for 30 years from two meteorological stations, with the aim of providing a more sustainable RWH system for water supply to private individuals, organizations, and government agencies. RWH is found to be technically feasible based on the prevailing rainfall pattern with over 90% of households having a rooftop constructed from technically appropriate materials. Results of the study indicate that an average roof of 80 m2 will collect 82,835 L/yr (45 L/person/day) for a family of five people which is about the required water demand for drinking and cooking purposes. Hence, the capacity of storage tanks and the catchment area required for an all-purpose water supply system based on RWH are quite large. These can be reduced to affordable sizes, by collecting and storing water for cooking and drinking only while non-potable uses are supplemented by water from other sources. However, it must be highlighted that due to the type of roofing material, rainwater should go through proper treatment in order to be used for potable purposes. This study clearly shows that Ibadan city has a good rainwater harvesting potential.
Effects of N-acetylcysteine on biofilm formation by MBR sludge
Song, WonJung,Lade, Harshad,Yu, YoungJae,Kweon, JiHyang Techno-Press 2018 Membrane Water Treatment Vol.9 No.3
N-acetylcysteine (NAC) has been widely used as an initial mucolytic agent and is generally used as an antioxidant to help alleviate various inflammatory symptoms. NAC reduces bacterial extracellular polymeric substances (EPS) production, bacterial adhesion to the surface and strength of mature biofilm. The efficacy has been shown to inhibit proliferation of gram-positive and gram-negative bacteria. In membrane bioreactor (MBR) processes, which contain a variety of gram negative bacteria, biofilm formation has become a serious problem in stable operation. In this study, use of NAC as an inhibitor of biofilm contamination was investigated using the center for disease control (CDC) reactors with MBR sludge. Biomass reduction was confirmed with CLSM images of membrane surfaces by addition of NAC, which was more efficient as the concentration of NAC was increased to 1.5 mg/mL. NAC addition also showed decreases in EPS concentrations of the preformed biofilm, indicating that NAC was able to degrade EPS in the mature biofilm. NAC addition was also effective to inhibit biofilm formation by MBR sludge, which consisted of various microorganisms in consortia.