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      • Action Models of Polydnaviral Factors in Translational Control

        Kyoungha Han,Yonggyun Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

        Viruses employ host translational machinery to synthesize their own proteins while negatively controling host protein translation. Endoparasitoid wasp (Cotesia plutellae) parasitizes young larvae of the diamondback moth (Plutella xylostella) larvae and possesses at least 27 genome segments. Two viral genes (CpBV15α and CpBV15 β) were obtained from cDNA library of the parasitized larvae showing no homology with any known polydnaviral genes. The parasitized larvae did not produce storage protein 1 (SP1) among at least three polypeptides (SP1, SP2, and SP3) at the stage of polydnaviral CpBV15β synthesis. When CpBV15β protein, which was expressed in Sf9 cells, was incubated with fat body isolated from nonparasitized larvae, SP1 synthesis was markedly inhibited. In vitro translation of mRNAs from nonparasitized larvae using rabbit reticulocyte lysate with CpBV15β significantly resulted in inhibition of SP1 synthesis, suggesting a negative role of CpBV15β in host protein synthesis.

      • KCI등재

        지역아동센터 아동의 심리 사회적 적응을 위한 집단미술치료 질적 사례 연구

        이경하(Kyoungha LEE),한경아(Kyeonga HAN) 한국조형교육학회 2018 造形敎育 Vol.0 No.67

        본 연구는 지역아동센터 아동이 심리 사회적 적응을 위한 집단미술치료 과정에서 무엇을 경험하고 그 의미가 무엇인지 알아 보는 데 목적이 있다. 참여 아동 6명의 집단을 구성하여 12회기의 집단미술치료를 실시하였으며, 아동, 교사, 부모 상담 및 관찰 통해 수집한 자료를 토대로 반복적 비교 분석법에 의하여 총 6개의 상위 범주, 16개의 하위 범주를 도출하고 그 의미를 파악하였다. 초기 서로를 신뢰하지 못하던 참여자들은 자유로운 미술표현을 통해 자신의 감정을 표출, 나를 객관화하 는 경험을 하였다. 이후 자신의 소망을 그림에 담으며 좀 개성 있는 나, 긍정적인 관계를 경험하며 심리 사회적으로 적응하는 모습을 보였다. 집단미술치료의 환경이 참여자들에게 창조적으로 문제를 해결하는 ‘잠재력’을 발현시키고, ‘서로를 존중하는 태도’를 갖는 데 치료적 역할을 했음을 알 수 있다. The purpose of the study is to find out the significance of a group of children’s experience as research participants in the group art therapy sessions for the psycho-social adjustment of them attending a community child center. To perform the research, 6 children were grouped and the 12-session group art therapy were executed. The significant meanings were extracted by collecting the recordings from session observation and counsels with the children, teachers and parents through the constant comparison method and organizing the data into 6 top categories and 16 subcategories. The participants who began the treatment without trusting each other experienced expressing their emotions and self-objectifying themselves. Also, they showed the improved psycho-social adjustments with experiencing the positive relationships while expressing their desires in the paintings. It showed that the group art therapy played the role of treatment by encouraging them to develop the ‘potential power’ which resolve problems creatively and supporting the communication with the ’attitude of mutual respect’.

      • KCI등재후보

        Temperature-Dependent Development of the Swallowtail Butterfly, Sericinus montela Gray

        Hong, Seong-Jin,Kim, Sun Young,Ravzanaadii, Nergui,Han, Kyoungha,Kim, Seong-Hyun,Kim, Nam Jung Korean Society of Sericultural Science 2014 International Journal of Industrial Entomology Vol.29 No.2

        The aim of this study is to investigate the effects of ambient thermal environments on the development of swallowtail butterflies (Sericinus montela Gray). Developmental durations and survival rates of S. montela were examined at two crucial developmental stages, embryonic and larval development, at varying temperatures ranging from $15^{\circ}C$ to $35^{\circ}C$. As expected, our results indicated that increasing temperatures decreased the developmental duration and survival rate of the eggs. However, the larvae and pupae showed maximum survival rates at $20.0^{\circ}C$ and $25.0^{\circ}C$, and the represented durations were similar to those of the eggs. Larval development was stage-specific, revealing that the fourth and fifth instars at the later stages were more susceptible to temperature variation. When considering both parameters, the optimal development of S. montela occurred within the temperature range of $20.0-25.0^{\circ}C$. The lower threshold for the complete development of S. montela from eggs to eclosion of adults was calculated at $10.6^{\circ}C$ by linear regression analysis. The estimated value is similar to that of other endemic insects distributed in temperate climate zones, which indicates that S. montela belongs to a small group of swallowtails adjusted to low ambient temperatures. From the results, we predict that the full development of S. montela could be achieved within the temperature range of $17.5-30.0^{\circ}C$. Embryonic development ceased at both test temperature extremes, and no further larval development proceeded after the third instar at $35.0^{\circ}C$. These results suggest that embryogenesis can be significantly influenced by slight variations in the ambient thermal environment that fall below the optimal range.

      • KCI등재후보

        Temperature-Dependent Development of the Swallowtail Butterfly, Sericinus montela Gray

        ( Seong Jin Hong ),( Sun Young Kim ),( Nergui Ravzanaadii ),( Kyoungha Han ) 한국잠사학회 2014 International Journal of Industrial Entomology Vol.29 No.2

        The aim of this study is to investigate the effects of ambient thermal environments on the development of swallowtail butterflies ( Sericinus montela Gray). Developmental durations and survival rates of S. montela were examined at two crucial developmental stages, embryonic and larval development, at varying temperatures ranging from 15℃ to 35℃. As expected, our results indicated that increasing temperatures decreased the developmental duration and survival rate of the eggs. However, the larvae and pupae showed maximum survival rates at 20.0℃ and 25.0℃, and the represented durations were similar to those of the eggs. Larval development was stage-specific, revealing that the fourth and fifth instars at the later stages were more susceptible to temperature variation. When considering both parameters, the optimal development of S. montela occurred within the temperature range of 20.0-25.0℃. The lower threshold for the complete development of S. montela from eggs to eclosion of adults was calculated at 10.6℃ by linear regression analysis. The estimated value is similar to that of other endemic insects distributed in temperate climate zones, which indicates that S. montela belongs to a small group of swallowtails adjusted to low ambient temperatures. From the results, we predict that the full development of S. montela could be achieved within the temperature range of 17.5-30.0℃. Embryonic development ceased at both test temperature extremes, and no further larval development proceeded after the third instar at 35.0℃. These results suggest that embryogenesis can be significantly influenced by slight variations in the ambient thermal environment that fall below the optimal range.

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