AN EXPERIMENTAL APPLICATION OF THE INFERENTIAL CONTROL SCHEME TO THE BINARY DISTILLATION COLUMN CONTROL

Lee, Won Kyoo,Kim, Kwang Eun 한국화학공학회 1984 Korean Journal of Chemical Engineering Vol.1 No.1

Immunomodulatory activity of phytoprotein isol Acanthopanax senticosus : Regulation of CTL responses and activation of macrop

Lee Seok Won,Lee Soo Jin,Park Jeon Ran,Kim Ha na,Ahn Kyoo Seok,Kim Jung-Hyo,Baek Nam-in,Kim Sung Hoon The Physiological Society of Korean Medicine and T 2004 동의생리병리학회지 Vol.18 No.1

We previously reported that the phytoprotein extracted from Acanthopanax senticosus (PA) had anti-carcinogenic anti-metastatic activity via increase of inhibition of gap junctional intercellular communication. In the present study investigated the immunomodulatory mechanism of phytoprotein isolated from the stem bark of Acanthopanax sentic (PA). PA was found to significantly stimulate macrophages producing TNF-α and IL-1β in vitro. It also showed tumori activity indicating that PA had the ability to stimulate macrophage directly. Moreover, PA induced the CDB/sup +/ CTL cy responses to recognize antigen on the B16-BL6 melanoma cells. Treatment of PA with B16-BL6 melanoma cells increased the proliferation of splenocytes compared with untreated control. These results demonstrate that PA immunomodulatory activity suggesting a useful anti-tumor agent applicable to treatment and prevention of cancer.

Hepatic Veno-Occlusive Disease Following Allogeneic Bone Marrow Transplantation For Leukemia Patients Conditioned With Busulfan And Cyclophosphamide : Incidence, Risk Factors and Clinical Outcome

Lee, Je-Hwan,Lee, Kyoo-Hyung,Kim, Tae-Won,Yun, Whan-Jung,Jung, Byung-Hak,Choi, Jong-Soo,Zang, Dai-Young,Kim, Sung-Bae,Kim, Sang-We,Suh, Cheolwon,Lee, Jung-Shin,Kim, Woo-Kun,Kim, Sang-Hee 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

간의 정맥폐색성질환 (veno-occlusive disease, VOD)은 황달, 통증을 동반하는 간장의 비대, 원인 미상의 체중 증가 및 복수를 동반하는 임상적 증후군으로서 골수이식술 전에 시행되는 고용량의 항암화학요법이나 방사선요법에 의하여 발생하는 질환이다. VOD의 발생 빈도는 보고자마다 차이가 있는데, 이것은 각각의 골수이식센터마다 투여하는 항암요법의 종류와 대상 환자군 및 위험 인자들이 다르기 때문이다. 저자 등은 1993년 12월 부터 1996년 10월 까지 서울중앙병원에서 busulfan 및 cyclophosphamide (BU-CY)의 투여 후 동종골수이식술을 시행 받은 24예의 백혈병 환자들을 대상으로 하여 VOD의 발생 빈도, 위험 요인 및 임상적 경과 등을 관찰하였다. 대상 환자들의 질환을 살펴보면 급성골수성백혈병 12예, 급성림프구성백혈병 5예, 만성골수성백혈병 6예 및 골수이형성증 1예 였다. 모든 환자들은 동종골수이식술 전에 busulfan 16 mg/kg 및 cyclophosphamide 120 mg/kg를 투여 받았으며, HLA가 일치하는 형제로 부터 골수를 공여받았다. 이식편대숙주질환을 예방하기 위하여 cyclosporine과 methotrexate를 투여하였고, 21예의 환자에서는 VOD 를 예방하기 위하여 heparin을 지속정주하였다. VOD는 임상적 기준으로 진단하였는데, 진단 기준은 (1) 혈중 빌리루빈 농도가 2 mg/㎗ 이상일 때, (2) 통증을 동반하는 간장의 비대가 있을 때, (3) 특별한 원인 없이 기저치의 2%가 넘는 체중의 증가 혹은 복수가 있을 때 등의 3가지 중에서 2가지 이상을 만족시키는 것으로 하였다. 12예(50%)의 환자에서 VOD가 발생하였는데 8예는 세가지 진단 기준을 모두 만족시켰으며, 4예는 2가지만 만족시키는 경우였다. VOD 발생 시기의 중앙치는 이식술 후 8일 이었으며, 중앙치 33일의 유병기간을 가졌다. 11예에서는 VOD로 부터 회복하였으며, 1예에서는 이식술 후 36일에 다기관 기능부전(multiorgan failure)으로 사망하였다. VOD의 발생에 영향을 미치는 위험인자를 분석해 보았을 때, 이식술 직전의 혈중 ALT치의 상승이 유일하게 독립적으로 영향을 미치는 인자였다. VOD 가 발생한 환자들은 다른 환자들에 비하여 혈소판 수혈 없이 2만/㎕까지 되는 기간이 유의하게 길었으며, 혈소판 수혈량이 유의하게 많았다. 이상의 결과로 BU-CY를 이용한 동종골수이식술 후 VOD의 발생율이 높음을 관찰하였으며, VOD발생의 위험인자로는 이식술 직전의 혈중 ALT치의 상승이 있었다. Veno-occlusive disease(VOD) of the liver is a clinical syndrome characterized by hyperbiliru-binemia, painful hepatomegaly and fluid retention. In bone marrow transplantation(BMT) setting, VOD is caused by dose-intensive chemotherapy and/or radiotherapy used to prepare patients for transplant. The incidence of VOD varies considerably from center to center, depending on cytoreductive therapy, the selection of the patients to be transplanted and other risk factors. The aim of this study is to determine the incidence, risk factors, and clinical outcome of hepatic VOD in leukemia patients conditioned with busulfan and cyclophosphamide (BU-CY) for allogeneic BMT. Twenty four leukemia patients underwent allogeneic BMT from December, 1993 to October, 1996 in Asan Medical Center. The underlying diagnoses were AML(12), ALL(5), CML(6) and myelodysplasia(1). The patients were prepared for BMT with busulfan (16 mg/kg) and cyclophosphamide (120 mg/kg). All patients received allogeneic marrow grafts from HLA identical siblings. Cyclosporine plus short course of methotrexate was given for GVHD prophylaxis and twenty one patients received heparin for VOD prophylaxis. VOD was clinically defined as having two of the following features: jaundice (bilirubin ≥ 2 mg/dL), tender hepatomegaly and/or unexplained weight gain( > 2% from baseline). VOD developed in twelve (50.0%) among twenty four patients. Eight patients exhibited the three standard clinical features of VOD and only two of the three features were present in fore patients. The median day of onset of VOD was day 8(range, 4 to 23) and the median duration of this complication was 33 days (range, 21 to 103). VOD has resolved in eleven of twelve patients and one patient died of multiorgan failure at day 36. When various individual characteristics such as sex, age, underlying disease, time to BMT from diagnosis, conditioning regimen, presence of hepatitis virus, the amount of pretransplant transfusion, history of liver disease, abnormal LFT at transplant, abnormal coagulation profile, and use of heparin, prophylaxis were analyzed, the only independent risk factor for the development of VOD was increased ALT level at the time of BMT. The platelet engraftment was significantly slower and platelet transfusion requirements were significantly higher in patients with VOD. In summary, our study showed high incidence of VOD in leukemia patients conditioned with BU-CY for allogeneic BMT and elevated ALT prior to BMT was an independent risk factor associated with the occurrence of VOD. In patients VOD, platelet engraftment was slower and platelet transfusion requirements were higher.

Politics and Church in Latin America : Focused on the Liberation Theology

Lee, Won Kyoo 전북대학교 사회과학연구소 1991 지역과 세계 Vol.18 No.-

<i>Rhus verniciflua</i> Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice

Lee, Joo-Ho,Lee, Hyo-Jung,Lee, Hyo-Jeong,Choi, Won-Cheol,Yoon, Seong-Woo,Ko, Seong-Gyu,Ahn, Kwang Seok,Choi, Seung-Hoon,Ahn, Kyoo-Seok,Lieske, John C.,Kim, Sung-Hoon Elsevier 2009 Phytomedicine Vol.16 No.2

<P><B>Abstract</B></P><P>Cisplatin-induced oxidative stress can cause liver and kidney damage, thus limiting therapeutic efficacy. Thus, in the present study, since <I>Rhus verniciflua</I> Stokes (RVS) containing flavonoids has antioxidant effects, we investigated whether it can protect cisplatin-induced toxicity <I>in vitro</I> and <I>in vivo</I>, The <I>in vitro</I> effects of RVS on the cell viability and reactive oxygen species (ROS) production were investigated using cisplatin-treated Madin–Darby Canine kidney (MDCK)-I renal cells. Its <I>in vivo</I> effects were also studied in BALB/c mice inoculated with CT-26 colon adenocarcinoma cells and treated with cisplatin with or without RVS. Liver and renal functions were assessed together with indices of tissue oxidation. RVS prevented cisplatin-induced cytotoxicity and ROS release against MDCK-I cells. RVS alone exerted modest antitumor activity against CT-26 cells. When used concurrently with cisplatin, RVS prevented the increases in serum blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and NO, while reducing liver and kidney tissue MDA content, and increasing catalase, glutathione (GSH), and superoxide dismutase (SOD) activities. Moreover, the antitumor efficacy of cisplatin was not altered by concurrent administration of RVS. These findings demonstrate that RVS prevents cisplatin-induced toxicity <I>in vitro</I> and <I>in vivo</I> via an antioxidant activity without hurting its antitumor effectiveness, suggesting that RVS can be usefully applied to the neoplastic patients as a combined chemopreventive agent with cisplatin.</P>

Functional Studies on the Interaction between Human Replication Protein A and Xeroderma pigmentosum Group A Complementing Protein (XPA)

Lee, Bong-Eun,Sung, Jae-Wook,Kim, Dong-Kil,Lee, Jong-Ryul,Kim, Nam-Deuk,Kang, Shin Won,Kim, Dong-Kyoo 부산대학교 유전공학연구소 1999 분자생물학 연구보 Vol.15 No.-

The human replication protein A (RPA; also known as human single-stranded DNA binding protein, HSSB) is a multisubunit complex (70, 34 and 11 kDa subunits) involved in the three processes of DNA metabolism; replication, repair, recombination. We found that both 34 and 70 kDa subunits (p34 and p70, respectively), of RPA interacts with the Xeroderma pigmentosum group A complementing protein (XPA), a protein that specifically recognizes UV-damaged DNA. Our mutational analysis indicated that no particular domains of RPA p70 were essential for its interaction with XPA. We also examined the effect of this XPA-RPA interaction on in vitro simian virus 40 (SV40) DNA replication catalyzed by the crude extract and monopolymerase system. XPA inhibited SV40 DNA replication in vitro through its interaction with RPA. Taken together, these results suggest that there is a roli for RPA in the regulation of DNA metabolism through its ability to modulate the interactions of proteins involved in the processes of DNA metabolism.

The Effect of the Territorial Diviion of Korean Peninsula on Party Politics

Lee, Won Kyoo 전북대학교 사회과학연구소 1999 지역과 세계 Vol.25 No.-

본 논문은 한반도의 분단이라는 특수한 상황이 한국의 정치 특히 정당정치와 선거에 미치는 영향을 중점적으로 분석하는데 그 목적이 있다. 이를 위하여 한반도의 분단이 한국민의 정치의식과 행태에 미친 영향을 먼저 분석하고, 정당정치와 한국인의 투표행태에 미치는 영향을 분석하면서 역사적으로 고찰한다. 특히 한국정당정치에서 진보정당이 자리 잡을 수 없었던 요인을 분단이라는 특수상황과 관련하여 역사적으로 고찰한다.

A Study on the Underdevelopment of Latin America

Lee, Won Kyoo 전북대학교 사회과학연구소 1992 지역과 세계 Vol.19 No.-

발전론자와 종속이론의 주창자 공히 그들의 초점은 변화와 도전에 있다. 물론 그들 사이에는 본질적으로 발전, 변화에 대하여 낙관적이냐 혹은 비관적이냐 또는 비판적이나 혹은 찬미적이냐의 관점의 차이가 존재하지만, 종속이론가들에 의하면 라틴아메리카를 포함한 제3세계국가들은 오늘날 경제적, 사회적, 정치적 저발전의 단계에서 정돈상태에 빠져있다고 한다. 그들에 의하면 한 국가의 발전은 서구 선진자본주의 국가들에 의한 세계의 식민지화와 더불어 나타난 전 세계적인 정치적, 경제적 체계들에 대한 역사적 투영과 관련되어질 때 비로서 파악되어질 수 있다는 것이다. 그리고 종속이론가와 세계체계이론가들 모두 발전론자의 모델 이 오늘날 일찍이 서구 선진국가들을 특징지웠던 그것과 아주 다른 종류의 경제적 경험과 시련을 겪고 있는 라틴아메리카를 포함한 제3세계국가들에 적용하기에는 부적합하다고 주장한다. 특히 종속이론가들은 발전론자의 정치발전이론보다 경제발전이론을 더 집중 공략하고 있으며, 이는 근대화 이론의 심장부를 겨냥하는 것과 같다. 더 나아가 그들은 근대화이론이 사회적 정치적 요인들은 정시한 채 협소하게 경제적 요인들만에 초점을 맞추고 있다고 비판한다. 본 논문은 라틴아케리카의 저발전에 관한 종속이론가들의 관점 뿐만 아니라 근대화론자, 생산양식이론가, 세계체계이론가, 구조 내지 제도론자들의 견해를 수용, 취사 선택하여 라틴 아메리카의 저발전을 종합적 관점에서 분석 고찰하고자 한다. 아울러 라틴 아메리카의 저발전 악순환의 굴레에서 벗어나기 위한 방안을 5가지의 시각에서 제시하고자 한다.

Prognostic implications of the immunophenotype in biphenotypic acute leukemia

Lee, Je-Hwan,Min, Yoo Hong,Chung, Chul Won,Kim, Byoung Kook,Yoon, Hwi-Joong,Jo, Deog-Yeon,Shin, Ho-Jin,Bang, Soo-Mee,Won, Jong-Ho,Zang, Dae Young,Kim, Hyeoung Joon,Chi, Hyun-Sook,Lee, Kyoo-Hyung,Cheon Informa UK (TaylorFrancis) 2008 Leukemia & lymphoma Vol.49 No.4

<P>The present study retrospectively analyzed clinicopathological and clinical data from 43 adult patients with biphenotypic acute leukemia (BAL) from 11 Korean institutes. The incidence of BAL was 2.1% among acute leukemias. In terms of immunophenotype, 31 patients had myeloid plus B-lymphoid (M + B), 10 had myeloid plus T-lymphoid (M + T), one had myeloid plus B-lymphoid plus T-lymphoid (M + B + T), and one had B-lymphoid plus T-lymphoid (B + T). Patients with M + T phenotype had significantly lower CR rate (55.6% vs. 88.0%, P = 0.039) and lower overall survival (0% vs. 33.9% at 5 years, P = 0.028) than those with M + B phenotype. Our results suggest that immunophenotype has prognostic implications in adult patients with BAL.</P>

옥수수 分蘖의 浸透率과 表現度

Won Koo Lee(李元九),Kwon Kyoo Kang(姜權圭),Hee Bong Lee(李喜鳳),Bong Ho Choe(崔鳳鎬) 한국육종학회 1989 한국육종학회지 Vol.21 No.3

In order to find the penetrance and the expressivity of genes associated with maize tillers among several Korean local maize lines, several inbred lines, their F₁s and F₂s were planted under different plant densities and counted the number of tillers per plant at maturity. All local lines with tillers showed 100% of penetrance regardless of planting densities and all F₁ plants crossed with IK line, which has tillers, showed also 100% of penetrate. The F₂ plants between IK and U.S.lines showed 40% to 60% of penetrance of tillering habits. The genes responsible for the tillering in IK seemed to be dominant in gene effects. The expressivities of tillering habits of IK line ranged from two to three and varied with planting densities. The expressvity of tillering habits of other maize lines ranged from two to ten. The expressivity of F₁ among Korean local lines showed quite uniform number of tillers per plant, while the F₁ between IK and U.S. lines showed very irregular number of tillers per plant.