L-carnitine supplementation improves the cryosurvival and subsequent development of bovine embryos

Kyeong Yeob Kim,Youn Bae Park,Byeong Ho Kim,Jin Hee Lee,Ji Hye Lee,Chanuka Kulatunga,Dong Eon Kim,Kyu Hyun Kim,Ryeong Eun Kim,Yoon Seok Nam,Min Kyu Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.

Establishment of a bone-specific col10a1:GFP transgenic zebrafish

Kim, Yong-Il,Lee, Suman,Jung, Seung-Hyun,Kim, Hyun-Taek,Choi, Jung-Hwa,Lee, Mi-Sun,You, Kwan-Hee,Yeo, Sang-Yeob,Yoo, Kyeong-Won,Kwak, SeongAe,Lee, Joon No,Park, Raekil,Choe, Seong-Kyu,Kim, Cheol-Hee Springer-Verlag 2013 Molecules and cells Vol.36 No.2

A New High-Yielding and Good-Quality Rice Cultivar "Sindongjinbyeo"

Bo Kyeong Kim,Hyun Tak Shin,Jae Kil Lee,Jae Kwon Ko,Bo Gab Yang,Tae Hwan Noh,Yang Ho Oh,Seung Yeob Lee,Ki Yong Ha,Jong Seok Kim,Jeong Kwon Nam,Hyun Jung Kang,Jong Cheol KoSoo 한국육종학회 2003 한국육종학회지 Vol.35 No.4

Sindongjinbyeo is a new japonica rice cultivar developed from a cross between Hwayeongbyeo and YR13604Acp22 line by the rice breeding team of National Honam Agricultural Experiment Station (NHAES), RDA, in 1999. This cultivar has a large grain and about 1

Comparison of the effects of FBS and BSA in hypothermic preservation of bovine (Hanwoo) embryos

Dong Eon Kim,Kyeong Yeob Kim,Eun Do Lee,Beom Sik Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Until recently, there have been many researches about the freezing methods and several methods of cryopreservation. Hypothermic preservation has been used to complement the embryo freezing technology. There is a study to show the successful results for long-term hypothermic preservation. For that reason, FBS and BSA are commonly added to the culture medium to support embryo development. We investigated the effectiveness of hypothermic preservation method at 4℃ according to embryonic developmental stages for Hanwoo embryos and evaluated the effect of FBS and BSA on Hanwoo embryos as a supplemental reagent in hypothermic preservation medium after recovering preserved embryos from hypothermic preservation. The present study reported that survival and hatching rates of embryos at morula stage following storage at 4℃ is Day 7 group was significantly higher (p < 0.05) compared than those of other groups (p < 0.05). As a result, the survival and hatching rates of embryos at the blastocyst stage following storage at 4℃ result is showed that significantly higher (p < 0.05) survival rates than those of other groups an Day 6. The result showed that hatching rate at Day 6 and 7 were significantly lower (p < 0.05) compared with other groups. The result regarding the survival and hatching rates of bovine embryos following storage at 4℃ for 72 h in various concentrations of BSA are shown The results showed that survival rate of 1% BSA group was not significantly different (p < 0.05) compare with control (FBS) group. Also, the results showed that hatching rate of control (FBS) and 1% BSA were significantly different (p < 0.05) compared with other groups. In conclusion, our result demonstrated that the hypothermic preservation did not effect on the survival and hatching rates of embryos after recovering. In addition, the supplementation of BSA in preservation medium showed no difference in the embryo developmental competence after hypothermic preservation compared to FBS treatment. With that, BSA can be an alternative reagent for the hypothermic preservation medium as an energy source and pH buffer.

도로와의 이격거리를 고려한 철도사업의 환경생태적 영향 최소화 방안에 대한 연구

김민경(Min Kyeong Kim),김동엽(Dong Yeob Kim) 응용생태공학회 2020 Ecology and resilient infrastructure Vol.7 No.3

철도와 도로는 대표적인 선형사업으로, 전국적으로 철도망과 도로망이 확대되고 있는 추세이며, 환경에 미치는 영향을 최소화하기 위해 철도와 도로의 평행노선에 따른 생태계 단절을 최대한 지양하고 있다. 본 연구에서는 우선적으로 철도와 도로개발시, 환경에 미치는 영향을 살펴보기 위한 각 사업의 중점평가항목에 대한 비교 ‧ 분석을 통해 차이점과 특징을 고찰하였다. 그 결과, 도로 개발은 철도사업과는 달리 효율적인 토지이용계획 수립에 대한 사항과 토양 보호를 중점평가항목으로 제시하고 있다. 본 연구에서는 철도 노선이 도로와 인접하게 될 때의 환경적 영향을 살펴보기 위한 구체적인 평가 항목과 기준이 제시되어 있지 않음에 따라, 관련 문헌을 검토하여 총 8개 평가 항목을 제시하였다. 특히, 계획의 적정성과 입지타당성을 살펴보기 위한 전략환경영향평가 단계에서 기존의 도로에 철도가 인접하게 될 때 미치는 환경적 영향을 사전에 검토할 수 있는 이격거리 기준을 제안하고자, 기 운영 중인 철도 노선을 대상으로 도로와의 이격거리를 살펴보았으며, 환경생태적 영향을 최소화하기 위해 검토해야 할 이격거리로 200 m 이내일 것을 제시하였다. 본 연구 결과는 철도와 도로가 인접할 때 환경생태적 영향을 최소화할 수 있는 대안노선을 검토하는 사업계획 단계에서 근거 자료로 활용될 수 있을 것이다. Railroads and roads are typical linear projects, and their networks are expanding nationwide. To minimize the impact of their development on the environment, ecological disconnection due to the parallel lines of railroads and roads is to be avoided as much as possible. In this study, to examine the environmental impact of railroad and road development, the characteristics of railroads and roads and the differences between them were examined through a comparative analysis of key evaluation items. Based on the result, the road development, unlike the railroad project, suggests items for establishing an efficient land use plan and soil protection as the key evaluation items. There are no specific evaluation items and criteria that can be used to examine the environmental impact of railroad lines located adjacent to roads. In this study, eight evaluation items were suggested by reviewing the relevant literature. In particular, in the strategic environmental impact assessment stage, which examines the adequacy and location feasibility of the plan, the minimum separation distance criteria were proposed to examine in advance the environmental impact of railroads located adjacent to the existing roads. The distances were examined for the previously operated routes, and a distance of less than 200 m was suggested to minimize the environmental impact. The results were compared with those in other studies and analyzed. The results of this study can be used as a basis for examining alternative routes that can minimize the environmental impact of adjacent railroads and roads.

로지스틱 회귀분석을 활용한 철도개발예정지 환경생태 평가

김민경(Min-Kyeong Kim),김동엽(Dong Yeob Kim) 한국철도학회 2018 한국철도학회 학술발표대회논문집 Vol.2018 No.10

철도폐선부지의 효율적 활용을 위한 평가기준 개발

김민경(Min-Kyeong Kim ),김동엽(Dong Yeob Kim) 한국철도학회 2017 한국철도학회 학술발표대회논문집 Vol.2017 No.05

친환경 교통 수단인 철도에 대한 투자 비중이 증가함에 따라 철도 고속화와 복선화 및 선형개량 사업, 철도노선의 교외지역 이설 등으로 폐선부지가 급속히 증가하고 있는 추세이다. 그러나 철도폐선부지가 방치되어 도시미관을 저해하는 문제가 발생됨에 따라 최근 지자체에서는 철도폐선부지의 활용방안을 모색하고 있다. 또한, 국토교통부는 「철도유휴부지 활용지침」을 제정하여 보전, 활용, 기타부지 등 3가지 유형으로 분류하여 폐선부지를 체계적으로 활용하고자 하였다. 그러나 현재 활용 중인 폐선부지는 공원, 레일바이크, 자전거도로, 태양광사업 등으로만 활용되고 있으며, 폐선부지의 다각화 및 사후평가에 대한 연구가 부재한 실정이다. 본 연구에서는 국내외 폐선부지의 활용사례를 조사하고 전국 철도폐선부지를 대상으로 자연문화경관, 교육과학적 가치, 위치 및 접근성, 휴양 유발성, 개발 여건 등 영향 요인을 분석하여 현재 철도폐선부지가 용도에 적합하게 활용되고 있는지를 분석하고 또한 용도폐기 대상 여부를 검토하였다. The increase in the investment to railroad which is an environment-friendly transportation measure vehicles boosted express railway, double track program, line form improvement project, and moving railroad routes into the suburbs. They resulted in fast increase of railway yards. However, as the railroad yards being neglected, urban fine sites have been degraded. Local governments kept seeking ways to utilize the railway yards. In addition, Ministry of land, infrastructure and transport enacted 「Guidelines for utilization of railway yards」. The Guidelines categorized the railway yards into three types; conservation, utilized, and other sites in order to make efficient use of them. The railway yards have been converted and used for parks, rail bike trails, bike paths, and solar projects. It seems that studies are needed on diverse use of the yards and on post evaluation. This study investigated current uses of railway yards, domestic and foreign, and analyzed for the pertinent conditions on natural and cultural landscape, educational value, location and accessibility, potential for recreational area, and development opportunities. Results were summarized to evaluate the railway yards for their fitness to the sites, and for the possibility of other uses.

AHP 기법을 이용한 철도개발예정지의 정량적 평가기준 개발

김민경(Min-Kyeong Kim),김동엽(Dong Yeob Kim) 한국철도학회 2017 한국철도학회 학술발표대회논문집 Vol.2017 No.10

우리나라 농경지에서 질소의 수계유출에 의한 아산화질소 간접배출량 평가

김건엽(Gun-Yeob Kim),정현철(Hyun-Cheol Jeong),김민경(Min-Kyeong Kim),노기안(Kee-An Roh),이덕배(Deog-Bae Lee),강기경(Kee-Kyung Kang) 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.6

아산화질소 (N₂O)는 가장 중요한 지구온난화 가스 중의 하나로 농업용으로 시용한 비료의 생물학적인 변환에 의한 것이 가장 큰 배출 원인으로 알려져 있다. 우리나라 농경지로부터 아산화질소의 간접배출 특성 및 배출량을 밝히기 위해 2007부터 2010년 까지 4년간 경남지방의 농업용관정의 지하수 질소성분들을 분석한 결과, 총질소의 경우 평균 6.91, 질산태질소는 5.06 mg-N L<SUP>-1</SUP>이었다. 용존 아산화질소의 농도는 평균 14.2 μg-N L<SUP>-1</SUP>, 중앙값은 7.8 μg-N L<SUP>-1</SUP>, 최고값은 169.6 μg-N L<SUP>-1</SUP>로 분포하였다 지하수 중의 총 질소나 질산태질소의 농도는 연도나 시기별로 큰 차이가 없었으나 용존 아산화질소 농도는 연도별로는 2009년에 시기별로는 8월이 가장 낮은 결과를 보였다. 우리나라 지하수 중의 N₂O-N 농도와 NO₃-N 농도비는 평균값이 0.0034, 중앙값이 0.0018이며, 95% 이상이 IPCC 가이드라인의 default 값인 0.015 이하에 분포하고 있어 IPCC가 1996 가이드라인의 농경지 지하유출에 의한 간접배출계수 (EF5-g) 0.015 N₂O-N/NO₃-N가 우리나라의 환경보다 너무 높게 선정된 것임을 알 수 있었다. 따라서 IPCC의 EF5-g default 값인 0.015 N₂O-N/NO₃-N 대신 우리나라 농경지 지하수 중의 N₂O-N와 NO₃-N 농도비의 평균값인 0.0034 N₂O-N/NO₃-N을 농경지 질소의 지하유출에 의한 아산화질소 간접배출량 평가를 위한 국가고유 배출계수 (EF5-g)로 제시코자 하며, 따라서 농경지에서 수계유출에 의한 아산화질소 전체의 간접 배출계수 (EF5-g+EF5-r+EF5-e)는 현재 우리나라의 국가 배출량평가에 사용되는 IPCC default 값 0.025 N₂O-N/ NO₃-N 대신 0.0134 N₂O-N/NO₃-N를 제시코자 한다. IPCC의 수계 간접 배출계수인 0.025 N₂O-N/NO₃-N을 적용하여 평가한 2008년 우리나라 농경지에서 질소의 수계 유출에 의한 아산화질소 배출량은 1,801,576톤 (CO₂-eq)이었으나 본 연구에서 제시한 배출계수인 0.0134 N₂O-N/NO₃-N을 적용할 경우 964,645톤 (CO₂-eq)으로 835,931톤 (CO₂-eq)의 온실가스 배출 감축효과를 얻을 수 있었다. 하지만 본 연구에서 제시한 배출계수는 질소 유출경로 중 가장 비중이 큰 지하침출에 의한 배출계수인 EF5-g 뿐으로 우리나라 농경지 아산화질소 간접배출량의 올바른 평가를 위해서는 앞으로 시용된 질소 중 수계를 통한 외부 유출 비율에 관한 default 값인 FracLEACH (30%), 표면수 유출계수인 EF5-r (0.0075 N₂O-N/ NO₃-N), 그리고 하천변을 통해 유출되는 계수인 EF5-e (0.0025 N₂O-N/NO₃-N )에 관한 추가적인 연구가 수행되어야 할 것이다. This experiment was conducted to measure concentration of dissolved N₂O in ground-water of 59 wells and to make emission factor for assessment of indirect N₂O emission at agricultural sector in agricultural areas of Gyeongnam province from 2007 to 2010. Concentrations of dissolved N₂O in ground-water of 59 wells were ranged trace to 196.6 μg-N L<SUP>-1</SUP>. N₂O concentrations were positively related with NO₃-N suggesting that denitrification was the principal reason of N₂O production and NO₃-N concentration was the best predictor of indirect N₂O emission. The ratio of dissolved N₂O-N to NO₃-N in ground-water was very important to make emission factor for assessment of indirect N₂O emission at agricultural sector. The mean ratio of N₂O-N to NO₃-N was 0.0035. It was greatly lower than 0.015, the default value of currently using in the Intergovernmental Panel on Climate Change (IPCC) methodology for assessing indirect N₂O emission in agro-ecosystems (IPCC, 1996). It means that the IPCC’ present nitrogen indirect emission factor (EF5-g, 0.015) and indirect N₂O emission estimated with IPCC’ emission factor are too high to use adopt in Korea. So we recommend 0.0034 as national specific emission factor (EF5-g) for assessment of indirect N₂O emission at agricultural sector. Using the estimated value of 0.0034 as the emission factor (EF5-g) revised the indirect N₂O emission from agricultural sector in Korea decreased from 1,801,576 ton (CO₂-eq) to 964,645 ton (CO₂) in 20-eq08. The results of this study suggest that the indirect Emission of nitrous oxide from upland recommend 0.0034 as national specific emission factor (EF5-g) for assessment of indirect N₂O emission at agricultural sector.

우리나라 농업용 하천수 수질의 기후변화 영향 및 취약성 평가

김민경(Min-Kyeong Kim),최순군(Soon-Kun Choi),엽소진(So-Jin Yeob),어진우(Jinu Eo),김명현(Myung-Hyun Kim) 한국토양비료학회 2022 한국토양비료학회 학술발표회 초록집 Vol.2022 No.10