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- 저자 : Kumar Paritosh (검색결과 3 건)
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Kumar Paritosh,Amarjeet Kumar Singh,Amita Kush Mehrotra,Deepak Pental,Pradeep Kumar Burma 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.6
Anther and tapetum-specific genes are important for understanding male gametophyte development, as well as for their use in the development of barnase/barstar-gene based male sterility and restorer system for hybrid seed production. An essential component of the system is the availability of tapetum-specific promoters. In the present study, anther-specific genes were identified in cotton using microarray-based differential expression analysis, some of which show expression specific to the anthers at a stage where tapetum tissue was fully developed. Validation of the identified genes using RT-PCR and in situ hybridization identified one novel gene (AEG—Anther Expressing Gene) encoding a putative lipid binding protein as having a tapetum-specific expression. Further, three paralogs of the gene were identified in the cotton genome out of which the gene AEG1 (Anther Expressing Gene1) was found to express in the tapetum layer. Analysis of transgenic plants developed in cotton using 1.5 Kb promoter region of the of AEG1 gene fused upstream to the reporter gene β-glucuronidase revealed a broad window of expression of the AEG1 promoter in the tapetum tissue from the tetrad stage of anthers till the degeneration of the tapetum cells. Low levels of expression were also observed in the root tissues. Expression was not observed in the stem and leaves. The broad window of expression of AEG1 promoter in the tapetum tissue makes it a suitable candidate for the expression of the barstar gene for effective fertility restoration in the barnase/barstar system.
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Babuta Priyanka,Paritosh Kumar,Deswal Renu 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4
S-nitrosylation is a well-known post-translational modification that modulates nitric oxide-dependent cell signaling. NADH-dependent S-nitrosoglutathione reductase (GSNOR) and NADPH-dependent thioredoxin reductase (NTR) enzymes are essential for nitric oxide/S-nitrosothiol (NO/SNO) homeostasis. GSNOR and NTR regulate denitrosylation by reducing S-nitrosoglutathione (GSNO) and thioredoxins, respectively. Genome-wide identification yielded 4 GSNOR and 12 NTR (4 each of NTRA, NTRB, and NTRC) genes in Brassica juncea. Syntenic relationship showed whole genome triplication (WGT) and tandem duplications. The phylogenetic analysis revealed clustering of BjGSNORs and BjNTRs with Arabidopsis homologs suggesting high sequence similarity within groups. Subcellular localization prediction suggested BjGSNOR localizes not only to the cytosol, but also to the Golgi apparatus and endoplasmic reticulum. BjNTRA and BjNTRB were localized in the cytoplasm and mitochondria, respectively, whereas BjNTRC localized in the chloroplast and nucleus. Several cis-acting elements involved in light responsiveness and expression analysis suggested the regulation of denitrosylation by light. The analysis of the promoter region also showed various phytohormone-regulated elements, suggesting the involvement of these enzymes in plant growth. Furthermore, GSNOR and NTR activities were higher in early growth stages. Differential spatial distributions of both the enzymes were observed with higher activity in hypocotyl in comparison with roots and cotyledons of the seedling. In flower, the highest activities were observed in carpel and least in stamens. Collectively, these findings provide an understanding of the structure, localization, and evolution of multiple copies of denitrosylases BjGSNOR and BjNTR, along with their possible roles in plant development.
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