http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kwon, Kil Koang,Yeom, Soo-Jin,Lee, Dae-Hee,Jeong, Ki Jun,Lee, Seung-Goo Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.1
<P><B>Abstract</B></P> <P>Successful utilization of cellulose as renewable biomass depends on the development of economically feasible technologies, which can aid in enzymatic hydrolysis. In this study, we developed a whole-cell biosensor for detecting cellulolytic activity that relies on the recognition of cellobiose using the transcriptional factor CelR from <I>Thermobifida fusca</I> and transcriptional activation of its downstream <I>gfp</I> reporter gene. The fluorescence intensity of whole-cell biosensor, which was named as cellobiose-detectible genetic enzyme screening system (CBGESS), was directly proportional to the concentration of cellobiose. The strong fluorescence intensity of CBGESS demonstrated the ability to detect cellulolytic activity with two cellulosic substrates, carboxymethyl cellulose and <I>p</I>-nitrophenyl β-D-cellobioside in cellulase-expressing <I>Escherichia coli</I>. In addition, CBGESS easily sensed crystalline cellulolytic activity when commercial Celluclast 1.5L was dropped on an Avicel plate. Therefore, CBGESS is a powerful tool for detecting cellulolytic activity with high sensitivity in the presence of soluble or insoluble cellulosic substrates. CBGESS may be further applied to excavate novel cellulases or microbes from both genetic libraries and various environments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel biosensor for detecting cellulolytic activity is developed using cellobiose-dependent transcriptional regulator. </LI> <LI> CBGESS shows quantitative response to cellobiose with hyper sensitivity and specificity. </LI> <LI> Cellulase activity including hydrolysis of crystalline cellulose is measured by CBGESS <I>via</I> intracellular fluorescence. </LI> </UL> </P>
권길광(Kil Koang Kwon),윤석민(Seok Min Yoon),최창호(Chang Ho Choi),정봉근(Bong Geun Jeong),이기원(Ki Won Lee),이동희(Dong Heui Yi),김형주(Hyung Joo Kim) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.6
본 연구에서는 개인용 컴퓨터(PC) 내부의 시료를 채취하여, 세균의 CFU와 곰팡이의 종류를 분석하였다. 시료는 대중 PC 이용시설(PC방), 대학실험실 및 대학 전산실습실의 PC 51대에서 채취하였다. CFU(colony forming unit)법을 이용한 세균수의 경우, 컴퓨터 총 작동시간이 증가할수록 PC 내부의 세균수는 증가하는 것으로 확인되었으며(r2 = 0.90), PC 내부 부품 중에서는 CPU 냉각 팬에서 가장 높은 수치로 확인되었다(평균 605 CFU/cm2). 곰팡이의 경우, 다수의 유해성을 지닌 곰팡이가 검출되었으며, 그 중 Aspergillus sp.와 Penicillium sp.가 가장 많은 비율로 존재함이 확인되었다. 또한 PC 내부에서 채취된 먼지에서는 mg 당 212 CFU의 세균이 발견되었다. 본 연구의 결과, PC 내부에는 여러 다양한 종류의 미생물이 존재한다는 것을 확인하였으며, 이 결과는 PC의 취급, 사용 및 정비 시 각종 미생물에 의한 감염의 가능성이 있다는 것을 나타내고 있다. Presence and distribution of bacteria and fungi in inner compartment of PCs(Personal Computers) were investigated. Samples for the analysis were collected from inside of PCs which had been used in various facilities including public computer facilities, laboratories and computer training rooms of a university. Total number of PC examined in this study was 51 each. When the total CFU(colony forming unit) of the inner compartment of the PCs was measured, the bacterial count was found to be dependent on the operation time(total running time) of PCs. When the distribution of bacteria in the inner compartment of PCs was estimated, CPU(Central Processing Unit) cooling fan area showed the highest bacterial concentration(average 605 CFU/cm2). In the case of the fungi, various opportunistic pathogens including Aspergillus sp. and Penicillium sp. were isolated and identified in the inner compartment of PCs. And the average of bacterial number in the dust collected from the PCs was 212 CFU/mg. These results indicated that handling of PC might have a risk of infection by the microorganism.