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        Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants

        Kibor, A.C.,Sumption, K.J.,Paxton, E.A. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.4

        The Immunoglobulin $IgG_1$ and $IgG_2$ isotype immune responses of domestic ruminants and mice to Cowdria. ruminantium live infection or by immunization with inactivated organisms were determined by the enzyme linked immunosorbent assay and Western blotting. Immunization of goats with inactivated elementary bodies (IEBs) led to a predominant $IgG_1$ isotype response. This indicated that a Th2 response was induced. After challenge, the IgG isotype responses were mixed whereby both $IgG_1$ and $IgG_2$ antibodies were detected. Two goats that survived virulent challenge had a predominant $IgG_2$ isotype response. In cattle live infection by natur l challenge or experiment led to a predominant $IgG_1$ isotype response. Immunization of cattle with IEBs however led to mixed IgG responses characterized by similar $IgG_1$ and $IgG_2$ ratios. In the mouse live infection led to a predominant $IgG_2$ isotype response. This indicated the mouse developed a true Th1 type cell mediated immune response when inoculated with live organisms. Immunization with inactivated organisms on the other hand led to a dominant $IgG_1$ response. It is evident from this work that the immune responses of ruminants and mice to C. ruminantium are different and that using mice as the experimental model for immune responses to Cowdria ruminantium. is not the appropriate.

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        Process Modelling of an Analytic Control Machine in Virtual Reality Platform

        Kipkemoi Rono,Jean Bosco Byiringiro,Edwell. T. Mharakurwa,Andrew Kibor 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.5

        While the combination of virtual reality (VR), industrial internet of things (IIoT) and digital twin (DT) can promote the integration of the physical world (real) and the digital world (virtual), one of the most important challenges that companies face is the choice of architecture. This study intends to utilize VR, IIoT, DT advantages and to reduce some adverse effects the constituent processes produce when they are individually applied. This bridges the existing gap between the physical and digital machines to enable near real-time monitoring control of the manufacturing process. In the realization of this paradigm shift, we use of IIoT infrastructures by adopting a bi-directional communication protocol for conveyance of data and information between the physical and virtual models in hardware in the loop or software in loop configuration. This study takes into account user case industrial application for the formation of silica scale and methods of reducing its production. In systems operating above pH 8.5, magnesium silicate is very likely to form due to the presence of magnesium hydroxide Mg(OH)2 and silicate (SiO4)4− ions. In this research work, the developed platform (VR, IIoT and DT) is used to remotely monitor and control the process that prevents silica scale formation by maintaining an acidic solution (pH < 6.7). A supervisory control is achieved using VR whereby instructions and commands sent to the physical station for execution. The data collected is stored in a data lake and is used to find the PID controller trends for pH 4 dosing and gain actionable insights. Analysis of the data is done by utilization of visualization schemes, diagrams and infographics. Results show the achievement of near real-time control (correlation coefficient at 99.92%) of a cyber-physical machine using VR by the adoption of bi-directional communication between the physical and virtual models in an immersive environment.

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