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강부희,Kenneth Racicot,Sarah J. Pilkenton,권영인,Emmanouil Apostolidis 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
This study investigates the carbohydratehydrolyzinginhibitory potential of blueberry extract oncarbohydrate-hydrolyzing enzymes and evaluates if theinhibitory activity is proanthocyanidin (PAC) or lowermolecular weight phenolic dependent. Freeze-dried blueberrypowder was extracted using acetone and subjected toC18 extraction (BAE). Low-molecular weight phenolics(BAE-LMW) and PACs (BAE-PAC) were separated fromBAE with gel filtration chromatography using LH-20 column. Total phenolic content, PAC content, and phenolicprofiles using HPLC, as well as rat a-glucosidase, sucrase,and maltase inhibitory activities, were determined for allsamples. The rat alpha-glucosidase inhibitory activity ofBAE (IC50 0.390 mg/mL TP basis) was enhanced in BAELMW(IC50 0.242 mg/mL TP basis) and reduced in BAEPAC(IC50 0.915 mg/mL TP basis). Similar trends wereobserved with maltase and sucrase inhibitory activities. Our findings suggest that blueberry acetone extract hasinhibitory activity on carbohydrate-hydrolyzing enzymesand this effect is dependent on LMWs rather than PAC.
Kang, Bou-Hee,Racicot, Kenneth,Pilkenton, Sarah J.,Kwon, Young-In,Apostolidis, Emmanouil The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
This study investigates the carbohydrate-hydrolyzing inhibitory potential of blueberry extract on carbohydrate-hydrolyzing enzymes and evaluates if the inhibitory activity is proanthocyanidin (PAC) or lower molecular weight phenolic dependent. Freeze-dried blueberry powder was extracted using acetone and subjected to $C_{18}$ extraction (BAE). Low-molecular weight phenolics (BAE-LMW) and PACs (BAE-PAC) were separated from BAE with gel filtration chromatography using LH-20 column. Total phenolic content, PAC content, and phenolic profiles using HPLC, as well as rat ${\alpha}$-glucosidase, sucrase, and maltase inhibitory activities, were determined for all samples. The rat alpha-glucosidase inhibitory activity of BAE ($IC_{50}$ 0.390 mg/mL TP basis) was enhanced in BAE-LMW ($IC_{50}$ 0.242 mg/mL TP basis) and reduced in BAE-PAC (I$IC_{50}$ 0.915 mg/mL TP basis). Similar trends were observed with maltase and sucrase inhibitory activities. Our findings suggest that blueberry acetone extract has inhibitory activity on carbohydrate-hydrolyzing enzymes and this effect is dependent on LMWs rather than PAC.