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Kavita Shah,Sareeta Nahakpam,Puneeta Singh 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
The direct immobilization of soluble peroxidase isolated and partially purified from shoots of rice seedlings in calcium algi-nate beads and in calcium agarose gel was carried out. Peroxidase was assayed for guaiacol oxidation products in pres-ence of hydrogen peroxide. The maximum specific activity and immobilization yield of the calcium agarose immobilized per-oxidase reached 2,200 U mg-¹ protein (540 mU cm-³ gel) and 82%, respectively. In calcium alginate the maximum activity of peroxidase upon immobilization was 210 mU g-¹bead with 46% yield. The optimal pH for agarose immobilized peroxidase was 7.0 which differed from the pH 6.0 for soluble peroxidase. The optimum temperature for the agarose immobilized per-oxidase however was 30°C, which was similar to that of soluble peroxidase. The thermal stability of calcium agarose immo-bilized peroxidase significantly enhanced over a temperature range of 30~60°C upon immobilization. The operational stabil-ity of peroxidase was examined with repeated hydrogen peroxide oxidation at varying time intervals. Based on 50% conver-sion of hydrogen peroxide and four times reuse of immobilized gel, the specific degradation of guaiacol for the agarose im-mobilized peroxidase increased three folds compared to that of soluble peroxidase. Nearly 165% increase in the enzyme protein binding to agarose in presence of calcium was noted. The results suggest that the presence of calcium, ions help in the immobilization process of peroxidase from rice shoots and mediates the direct binding of the enzyme to the agarose gel and that agarose seems to be a better immobilization matrix for peroxidase compared to sodium alginate.
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Londhe Sanjay Bhalchandra,Shah Ravi Vinod,Patwardhan Meghana,Doshi Amit Pankaj,Londhe Shubhankar Sanjay,Subhedar Kavita,Kundnani Vishal G.,Patel Jwalant Y. 대한척추외과학회 2021 Asian Spine Journal Vol.15 No.6
Study Design: This is a prospective cohort study involving patients who were followed for 2 years after total knee replacement (TKR) to determine changes in lumbar spine and knee symptoms. Purpose: The objectives of this study were to determine the percentage of patients undergoing bilateral TKR who present with coexisting lumbar spine problems and determine if TKR relieves lumbar spine symptoms. Overview of Literature: No studies quantify the percentage of TKR patients who experience relief of lumbar spine symptoms after TKR surgery. Methods: The study included 200 patients (164 females, 36 males) undergoing primary TKR. Follow-up was performed at 4 weeks, 3, 6, 12, and 24 months. Lumbar spine and knee symptom improvements were assessed using the Oswestry Disability Index (ODI) and Oxford Knee Score, respectively. Results: All 200 patients undergoing bilateral TKR presented with radiographic lumbar spine degenerative pathology; 60% (n=120) of the patients presented with moderate to severe clinical symptoms of lumbar spondylosis, including 54% (n=108) with degenerative lumbar spondylosis and lumbar canal stenosis and 6% (n=12) with degenerative spondylolisthesis. Of the 120 patients who presented with lumbar spine problems, 90% (n=108) reported improvement in their symptoms; the ODI score improved from 42.5%±4.1% preoperative score to 15.6%±2.3% postoperative score (p-value<0.001). Of the 12 patients with no improvement, 10 patients underwent percutaneous procedures for their lumbar spine pathology with good results, one patient underwent surgery, and one declined any intervention. Conclusions: A significant number of patients (60%) undergoing bilateral TKR also present with symptomatic lumbar spine problems. Patients with mild to moderate lumbar spine degenerative symptoms and no associated severe radiating pain on activity are more likely to experience relief of their symptoms post-TKR.
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