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Cast Shadow Detection and Removal of Moving Objects from Video Based on HSV Color Space
Kaushik Kaushik,Deb, Animesh Kar,Ashraful Huq Suny 한국산학기술학회 2015 SmartCR Vol.5 No.1
In the process of segmentation and tracking of moving object, shadow area leads to false detection of object. Shadows are also reason for loss of background texture and false connectivity of independent blobs. Hence, we propose a simple method to detect a moving object’s cast shadow and remove the shadow region from video frames. Initially, we store all the background information in reference frame. The next incoming frames with object are compared with this frame. In order to extract the moving object, we used subtraction algorithm. We used homogeneity property by image division, color variation in RGB color space and statistics of intensity in V channel of HSV color space to detect the shadow region. Finally shadow removal is done based on the information from the reference frame. The most noticeable feature of our proposed method is that it can detect shadows both in indoor and outdoor scenarios and there is no harsh transition after removal of the shadow. Color information for both background subtraction and shadow detection to improve object segmentation is ensured in this paper. Experimental results show that the proposed method is simple to understand, can detect and remove shadow and extract the moving object properly.
Biological and medical applications of plasma-activated media, water and solutions
Kaushik, Nagendra Kumar,Ghimire, Bhagirath,Li, Ying,Adhikari, Manish,Veerana, Mayura,Kaushik, Neha,Jha, Nayansi,Adhikari, Bhawana,Lee, Su-Jae,Masur, Kai,von Woedtke, Thomas,Weltmann, Klaus-Dieter,Choi De Gruyter 2019 Biological chemistry Vol.400 No.1
<P><B>Abstract</B></P><P>Non-thermal atmospheric pressure plasma has been proposed as a new tool for various biological and medical applications. Plasma in close proximity to cell culture media or water creates reactive oxygen and nitrogen species containing solutions known as plasma-activated media (PAM) or plasma-activated water (PAW) - the latter even displays acidification. These plasma-treated solutions remain stable for several days with respect to the storage temperature. Recently, PAM and PAW have been widely studied for many biomedical applications. Here, we reviewed promising reports demonstrating plasma-liquid interaction chemistry and the application of PAM or PAW as an anti-cancer, anti-metastatic, antimicrobial, regenerative medicine for blood coagulation and even as a dental treatment agent. We also discuss the role of PAM on cancer initiation cells (spheroids or cancer stem cells), on the epithelial mesenchymal transition (EMT), and when used for metastasis inhibition considering its anticancer effects. The roles of PAW in controlling plant disease, seed decontamination, seed germination and plant growth are also considered in this review. Finally, we emphasize the future prospects of PAM, PAW or plasma-activated solutions in biomedical applications with a discussion of the mechanisms and the stability and safety issues in relation to humans.</P>
Kaushik, Nagendra Kumar,Kaushik, Neha,Min, Booki,Choi, Ki Hong,Hong, Young June,Miller, Vandana,Fridman, Alexander,Choi, Eun Ha IOP 2016 Journal of Physics. D, Applied Physics Vol.49 No.8
<P>The present study aims at studying the anticancer role of cold plasma-activated immune cells. The direct anti-cancer activity of plasma-activated immune cells against human solid cancers has not been described so far. Hence, we assessed the effect of plasma-treated RAW264.7 macrophages on cancer cell growth after co-culture. In particular, flow cytometer analysis revealed that plasma did not induce any cell death in RAW264.7 macrophages. Interestingly, immunofluorescence and western blot analysis confirmed that TNF-<I>α</I> released from plasma-activated macrophages acts as a tumour cell death inducer. In support of these findings, activated macrophages down-regulated the cell growth in solid cancer cell lines and induced cell death <I>in vitro</I>. Together our findings suggest plasma-induced reactive species recruit cytotoxic macrophages to release TNF-<I>α</I>, which blocks cancer cell growth and can have the potential to contribute to reducing tumour growth <I>in vivo</I> in the near future.</P>
Soft, self-assembled liquid-crystalline nanocomposite for superior switching
Kaushik Pal,Sami Sajjadifar,Mohamed Abd Elkodous,Yakubu Adekunle Alli,Fernando Gomes,Jaison Jeevanandam,Sabu Thomas,Alexander Sigov 대한금속·재료학회 2019 ELECTRONIC MATERIALS LETTERS Vol.15 No.1
Liquid crystal (LC) has long been a feature in Materials Science and Nanotechnology, have recently been extended into theappealing domain of complex hybrid materials. The crystalline structural eff ects of alkoxy chain lengths and the mesogenproperties of hydrogen-bonded (n-OBASA) complexes (n = 5,6,7) have been investigated in recent studies. The LC-basedhybrid nanocomposite materials–obtained by the homogeneous dispersion of zinc oxide nanowires (ZnO NWs) as a dopantinto hydrogen-bonded liquid-crystalline compounds—seem to be particularly promising in this article. Optimizing thegeometry of surface stabilizing electro-optic, LC cell reveals the typical intermolecular hydrogen bond (H-bond) formation. Here, we explore molecular-colloidal hybrid composite matrix formed from LCs and dilute dispersions of orientation-orderedZnO NWs, for eventual potential application in smart switchable display devices. In addition, we investigated the structural,dielectric and optical properties of the nanocomposite, and electro-optical studies which were performed by exploiting thepotential during the conditions before the opening of spectrum acquisition. Our novel fi ndings confi rm that the electric fi eldinduces a charge transfer of the LC molecules to the nanomaterial, which acts as a trap for ionic charges. This eff ect maybe utilized to achieve superior switching operation that is electro-optically tunable. Such dynamic novel switching could beharnessed in smart LCD technology and pave the way towards innovative display modulation techniques.
Stable Fuzzy Controller Design Employing Group Improvisation based Harmony Search Algorithm
Kaushik Das Sharma 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.5
The present paper proposes a group improvisation based variant of harmony search (HS) algorithm, for solving optimization problems, conceptualizing the mutual cooperation of philharmonic orchestra. The proposed conception of meta-heuristic optimization is aimed to bring the effects of local neighborhood topological model (lbest model) to the HS algorithm as found, particularly, in swarm based optimizations. This variant of HS employs a novel cooperative method for generating new solu-tion vectors that enhances the accuracy and convergence rate of harmony search (HS) algorithm. The proposed variant of HS algorithm has been successfully applied to design stable fuzzy controllers, op-timizing both its structures and free parameters, so that the designed controller can guarantee desired stability and simultaneously it can provide satisfactory performance with a high degree of automation in the design process. The variant and the original HS algorithm are implemented for two nonlinear benchmark systems in simulation case study and their results demonstrate that the proposed variant outperforms the original HS algorithm.