Genome Profiling for Health Promoting and Disease Preventing Traits Unraveled Probiotic Potential of Bacillus clausii B106

Kapse, N.G.,Engineer, A.S.,Gowdaman, V.,Wagh, S.,Dhakephalkar, P.K. The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.4

Spore-forming Bacillus species are commercially available probiotic formulations for application in humans. They have health benefits and help prevent disease in hosts by combating entero-pathogens and ameliorating antibiotic-associated diarrhea. However, the molecular and cellular mechanisms of these benefits remain unclear. Here, we report the draft genome of a potential probiotic strain of Bacillus clausii B106. We mapped and compared the probiotic profile of B106 with other reference genomes. The draft genome analysis of B106 revealed the presence of ADI pathway genes, indicating its ability to tolerate acidic pH and bile salts. Genes encoding fibronectin binding proteins, enolase, as well as a gene cluster involved in the biosynthesis of exopolysaccharides underscored the potential of B106 to adhere to the intestinal epithelium and colonize the human gut. Genes encoding bacteriocins were also detected, indicating the antimicrobial ability of this isolate. The presence of genes encoding vitamins, including Riboflavin, Folate, and Biotin, also indicated the health-promoting ability of B106. Resistance of B106 to multiple antibiotics was evident from the presence of genes encoding resistance to chloramphenicol, ${\beta}$-lactams, Vancomycin, Tetracycline, fluoroquinolones, and aminoglycosides. The findings indicate the significance of B. clausii B106 administration during antibiotic treatment and its potential value as a probiotic strain to replenish the health-promoting and disease-preventing gut flora following antibiotic treatment.

Genome Profiling for Health Promoting and Disease Preventing Traits Unraveled Probiotic Potential of Bacillus clausii B106

N.G. Kapse,A. S. Engineer,V. Gowdaman,S. Wagh,P. K. Dhakephalkar 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.4

Spore-forming Bacillus species are commercially available probiotic formulations for application in humans. They have health benefits and help prevent disease in hosts by combating entero-pathogens and ameliorating antibiotic-associated diarrhea. However, the molecular and cellular mechanisms of these benefits remain unclear. Here, we report the draft genome of a potential probiotic strain of Bacillus clausii B106. We mapped and compared the probiotic profile of B106 with other reference genomes. The draft genome analysis of B106 revealed the presence of ADI pathway genes, indicating its ability to tolerate acidic pH and bile salts. Genes encoding fibronectin binding proteins, enolase, as well as a gene cluster involved in the biosynthesis of exopolysaccharides underscored the potential of B106 to adhere to the intestinal epithelium and colonize the human gut. Genes encoding bacteriocins were also detected, indicating the antimicrobial ability of this isolate. The presence of genes encoding vitamins, including Riboflavin, Folate, and Biotin, also indicated the health-promoting ability of B106. Resistance of B106 to multiple antibiotics was evident from the presence of genes encoding resistance to chloramphenicol, β-lactams, Vancomycin, Tetracycline, fluoroquinolones, and aminoglycosides. The findings indicate the significance of B. clausii B106 administration during antibiotic treatment and its potential value as a probiotic strain to replenish the health-promoting and disease-preventing gut flora following antibiotic treatment.

Characteristics of high sensitivity ethanol gas sensors based on nanostructured spinel Zn1−xCoxAl2O4

S.D. Kapse,F.C. Raghuwanshi,V.D. Kapse,D.R. Patil 한국물리학회 2012 Current Applied Physics Vol.12 No.1

Nanocrystalline powders of Zn1-xCoxAl2O4 (x ¼ 0, 0.2, 0.4, 0.6, 0.8, 1.0) mixed oxides, with cubic spinel structure were successfully prepared by the ethylene glycol mediated citrate sol-gel method. The structure and crystal phase of the powders were characterized by X-ray diffraction (XRD) and microstructure by transmission electron microscopy (TEM). X-ray diffraction results showed that the samples were in single phase with the space group Fd-3m. TEM analysis showed that the powders with spherical shape were uniform in particle size of about 17e24 nm with mesoporous in nature. Further investigations were carried out by FT-IR. Thick films of as-prepared Zn1-xCoxAl2O4 powders were fabricated using screen-printing technique. The response of Zn1-xCoxAl2O4 based thick films towards different reducing gases (liquefied petroleum gas, hydrogen, hydrogen sulfide, ethanol gas and ammonia) was investigated. The sensor response largely depends on the composition, temperature and the test gas species. The Co (cobalt) content has a considerable influence on the gas-sensing properties of Zn1-xCoxAl2O4. Especially,Zn0.4Co0.6Al2O4 composition exhibited high response with better selectivity to 100 ppm C2H5OH gas at 150 ℃. The instant response (w7 s) and fast recovery (w16 s) are the main features of this sensor. Nanocrystalline powders of Zn1-xCoxAl2O4 (x ¼ 0, 0.2, 0.4, 0.6, 0.8, 1.0) mixed oxides, with cubic spinel structure were successfully prepared by the ethylene glycol mediated citrate sol-gel method. The structure and crystal phase of the powders were characterized by X-ray diffraction (XRD) and microstructure by transmission electron microscopy (TEM). X-ray diffraction results showed that the samples were in single phase with the space group Fd-3m. TEM analysis showed that the powders with spherical shape were uniform in particle size of about 17e24 nm with mesoporous in nature. Further investigations were carried out by FT-IR. Thick films of as-prepared Zn1-xCoxAl2O4 powders were fabricated using screen-printing technique. The response of Zn1-xCoxAl2O4 based thick films towards different reducing gases (liquefied petroleum gas, hydrogen, hydrogen sulfide, ethanol gas and ammonia) was investigated. The sensor response largely depends on the composition, temperature and the test gas species. The Co (cobalt) content has a considerable influence on the gas-sensing properties of Zn1-xCoxAl2O4. Especially,Zn0.4Co0.6Al2O4 composition exhibited high response with better selectivity to 100 ppm C2H5OH gas at 150 ℃. The instant response (w7 s) and fast recovery (w16 s) are the main features of this sensor.

Acetone gas-sensing performance of Sr-doped nanostructured LaFeO_3 semiconductor prepared by citrate sol―gel route

P.A. Murade,V.S. Sangawar,G.N. Chaudhari,V.D. Kapse,A.U. Bajpeyee 한국물리학회 2011 Current Applied Physics Vol.11 No.3

Nanocrystalline La_(1-x)Sr_xFeO_3 (x = 0, 0.1, 0.2, 0.3 and 0.4) was prepared by sol―gel citrate method. The structural and microstructural characterization has been carried out with the help of X-ray diffraction (XRD)and transmission electron microscopy (TEM), respectively. XRD studies confirm the formation of LaFeO_3with perovskite phase. Further studies by Fourier transform infrared spectroscopy were also conducted. DC electrical properties of the prepared nanoparticles were studied by D.C. conductivity measurements. The gas-sensing performance of the as-prepared La_(1-x)Sr_xFeO_3 nanoparticles was investigated towards different reducing gases like acetone (CH_3COCH_3), ethanol (C_2H_5OH), hydrogen sulfide (H_2S), hydrogen (H_2) and liquefied petroleum gas (LPG). The nanocrystalline La_0.7Sr_0.3FeO_3 exhibited good sensing performance towards acetone gas with rapid response and high sensitivity at 275 ℃ as compared with LaFeO_3.

Numerical investigation on subcooled boiling heat transfer coefficient of waterethanol mixture by CISCAM technique

Suhas Badakere Gopalakrishna,Chidanand Kishor Mangrulkar,Kiran Kumar Kapse Umashankar,Sathyabhama Alangar 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.4

The subcooled flow boiling (SFB) of a water-ethanol mixture are relevant in operating heat-dissipating devices, such as smaller catalytic reactors, electronic apparatus, and hybrid electric vehicle battery components. The operative temperature should always be at a sustainable value to evade the failure or breakdown of these heat-dissipating devices. To cool these devices, a water-ethanol mixture is used as a coolant. The forced convective as well as SFB heat transfer coefficients (HTCs) for the water-ethanol mixture are estimated numerically using the volume of fluid method in a rectangular channel with dimensions of 15 mm×15 mm× 150 mm. During SFB, the liquid-vapor interaction is examined by solving the bubble void fraction (BVF). For the discretization process, the Crank-Nicholson implicit method (scheme) is used, and the convective equation for the BVF is converted to an algebraic equation. The corrector predictor equation procedure is used for solving the BVF. The thermodynamic and thermophysical parameters related to subcooled boiling are estimated upon the incorporation of the bubble void fraction (α) using the mixture rule. These parameters are then incorporated into the x-momentum equation as well as into the energy equation for finding the fluid temperature, velocity, and pressure drop values. From the estimated values of temperature, subcooled flow boiling HTC is obtained. The estimated values of HTC can predict well compared with that of empirical equations. Moreover, mass flux plays a vital role in the forced convective region, while heat flux has a crucial role in the SFB region for the improvement of HTC.