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RISS 인기검색어
- 검색키워드
- 저자 : Kamaraj Chinnaperumal (검색결과 2 건)
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Kamaraj, Chinnaperumal,Gandhi, Pachiyappan Rajiv,Elango, Gandhi,Karthi, Sengodan,Chung, Ill-Min,Rajakumar, Govindasamy Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.107 No.1
<P><B>Abstract</B></P> <P>The future of this study was to prepare a natural pesticide which will not harm the environment and yet control pests. Neem gum nano formulation (NGNF), a novel biopesticide prepared from the Neem gum extract (<I>Azadirachta indica</I>) (NGE) was evaluated for its antifeedant, larvicidal and pupicidal activities against <I>Helicoverpa armigera</I> (Hub.) and <I>Spodoptera litura</I> (Fab.) at 100ppm. The NGNF showed significant (100%) antifeedant, larvicidal and pupicidal activities against <I>H. armigera</I> and <I>S. litura</I>. The LC<SUB>50</SUB> values of 10.20, 12.49 and LC<SUB>90</SUB> values of 32.68, 36.68ppm on <I>H. armigera</I> and <I>S. litura</I>, respectively at 100ppm. The NGNF treatments showed differences in the activities of detoxifying enzymes, carboxylesterases, glucosidases and glutathione S-transferases in the larval gut. Earthworm toxicity illustrated that 6.25ppm of chemical insecticides (cypermethrin) varied widely in their contact toxicities compared to 100ppm of NGNF and control in both contact filter paper and artificial soil test. The NGNF were characterized and confirmed by FTIR, XRD, SEM and EDX analysis. Ten compounds were identified from the Neem gum extract (NGE) by Gas Chromatography-Mass Spectrometry (GC–MS) analysis. The major compounds were fatty acids like Hexadecanoic acid, oleic acid, and ricinoleic acid. NGNF could be used as an agent to prepare novel bio-pesticides formulations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Neem gum mediated nano formulation (NGNF) as a bio-pesticide agent against <I>Helicoverpa armigera</I> and <I>Spodoptera litura</I>. </LI> <LI> NGNF exhibited potential antifeedant, larvicidal and pupicidal activities against <I>Helicoverpa armigera</I> and <I>Spodoptera litura</I>. </LI> <LI> Environmental toxicity was assessed by earthworm (<I>Eudrilus eugeniae</I>) there is no significant toxicity observed in NGNF treatments. </LI> </UL> </P>
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Kamaraj Chinnaperumal,Naveenkumar Selvam,Prem Pradisha,Ragavendran Chinnasamy,Subramaniyan Vetriselvan,Al-Ghanim Khalid A.,Malafaia Guilherme,Nicoletti Marcello,Govindarajan Marimuthu 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
Green nanoparticles represent a sustainable and eco-friendly approach to developing larvicides targeted at controlling mosquito populations. These nanoparticles are synthesized using natural, non-toxic materials and environmentally benign processes, emphasizing their potential to combat mosquito larvae while minimizing adverse effects on the ecosystem. This approach aligns with the growing need for sustainable solutions in vector control, providing an effective alternative to traditional larvicides with reduced environmental impact. The aim of the present investigation we have utilized the leaf from Laureliopsis philippiana (LP) to synthesize silver (Ag) and palladium (Pd) nanoparticles (NPs) and studied toxicity on deadly disease vectors larvae of Aedes aegypti and Anopheles stephensi. Biosynthesized Ag and Pd NPs were extensively characterized through physical and chemical methods. Gas chromatography–mass spectrometry analysis of L. philippiana assisted in identifying the secondary metabolites of the extract’s total flavonoid and phenolic compounds, which are in the reactants of the bioreduction process during the production of NPs. The in-silico analysis was conducted on mosquito sterol car rier protein-2 (1PZ4) and mosquito odorant binding protein (2L2C). The biosynthesized Ag and Pd NPs revealed the UV absorption peak at 417 and 370 nm, and X-ray diffraction results showed the crystalline structure of NPs. At the same time, in transmission electron microscopy representation, the Ag and Pd NPs were spherical, clus tered, and exhibited a noticeable average size of Ag NPs: 13.25 ± 2.53 nm and Pd NPs: 4.07 ± 1.64 nm. The biogenic Ag and Pd NPs showed concentration-dependent larvicidal mortality at 12 and 24-hour exposure with LC 50 values of 29.596, 17.804, and 23.915, 14.878 µg/mL on Ae. aegypti and 23.946, 13.938, 19.457, and 12.876 µg/mL against An. stephensi, correspondingly. In-silico studies proved that Laureliopsine A and isoterpinolene showed significant docking scores compared to permethrin on mosquito proteins (1PZ4 and 2L2C). Besides, the LP-Ag and Pd NPs non-toxicity analysis was carried out on zebrafish (Danio rerio) embryos at 72 h post- * Corresponding authors. E-mail addresses: kamarajc@srmist.edu.in (C. Kamaraj), guilhermeifgoiano@gmail.com (G. Malafaia). https://doi.org/10.1016/j.aspen.2023.102164 Received 31 August 2023; Received in revised form 9 October 2023; Accepted 11 October 2023 Available online 13 October 2023 1226-8615/© 2023 Korean Society of Applied Entomology. Published by Elsevier B.V. All rights reserv
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