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Wei Zhang,Kaiqi Lian,Fan Yang,Yang Yang,Zhijian Zhu,Zixiang Zhu,Weijun Cao,Ruoqing Mao,Ye Jin,Jijun He,Jianhong Guo,Xiangtao Liu,Haixue Zheng 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3
Integrin anb3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and rolesof anb3 integrin, a stable CHO-677 cell line expressing the murine anb3 heterodimer (designated as “CHO-677-manb3” cells) wasestablished using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits an and b3 were detected at the gene andprotein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-manb3 cellline at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed stably. A plaque-formingassay, 50% tissue culture infective dose (TCID50), real-time quantitative reverse transcription-PCR, and IFA were used to detect the replicationlevels of Foot-and-mouth disease virus (FMDV) in the CHO-677-manb3 cell line. After infection with FMDV/O/ZK/93, the cell line showeda significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stableanb3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of anb3integrin, and as a cell model for FMDV research.
Jiayong Qiu,Shui Yu,Jiugang Shao,Kaiqi Zhu,Dianchun Ju,Chunyu Chen,Dexing Qi,Fei Wang,Ni Bai,Rui Mao,Xiaoming Wang 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.4
A high basicity charge prepared with electric arc furnace dust (EAFD), carbonaceous reducing agent and CaO is proposed. The mechanisms of enhancing separation of zinc and iron by calcified carbothermic reduction of the high basicity charge were analyzed by combining thermal analysis kinetics and experiment. The influences of roasting temperature, carbon ratio (nc/no, molar ratio of carbon in graphitic carbon powder to oxygen in EAFD), and CaO dosage on phase transition and dezincification ratio in EAFD were investigated. The results show that the intermediates Ca2Fe2O5 and Fe0.85−xZnxO can be produced from the zinc-iron separation of zinc ferrate during the process of calcified carbothermic reduction of EAFD. Addition of CaO and C results in the following transition pathways: ZnFe2O4+ CaO→Ca2Fe2O5+ZnO→Ca2Fe2O5+Zn(g)→CaO+Fe; Fe0.85−xZnxO+CaO→Ca2Fe2O5+FeO+ZnO→CaO+Fe+Zn(g). In the range of nc/no of 0.4–1.2 and roasting temperature of 1,000–1,100 °C, the addition of CaO can promote reduction and dezincification. Based on the Kissinger-Akahira-Sunose (KAS) and Coats-Redfern methods, the kinetic results show that the calcified carbothermic reduction process can be divided into three stages: initial stage (α=0–0.3), middle stage (α=0.3–0.45), and final stage (α=0.45–1.0). The average activation energy of the initial stage is 305.01 kJ·mol−1, and the reaction mechanism is one-dimensional diffusion. The average activation energy is 315.67 kJ·mol−1 for the middle stage and 288.22 kJ·mol−1 for the final stage. The chemical reaction equation is found to be the most suitable mechanism in the medium and final stages. It is also found that the addition of CaO can reduce the average activation energy by about 32 kJ·mol−1 and shorten the intermediate stage of the reaction.