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Junbo Zuo,Hong Zhou,Jiazeng Xia,Feng Ju,Jiang Yan,Akao Zhu,Shimao Jin,Ting Shan 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.4
Underexpression of the gene runt-related transcription fac-tor 3 (RUNX3), an important tumor suppressor, is known to contribute to gastric cancer progression. However, the mechanism underlying aberrant RUNX3 expression has not been fully elucidated. We investigated the role of microRNA-148a (miR-148a) and DNA methyltransferases (DNMTs) in RUNX3 promoter methylation and gene expression. RUNX3 mRNA, RUNX3 protein, and methylation levels were assayed in human gastric cancer tissues and matched normal tissues, and AGS and BGC-823 cells by real-time reverse transcription PCR, Western blot, and methylation-specific PCR, respectively. A correlation between RUNX3 mRNA levels and that of miR-148a was also investigated in gastric cancer tissues. We found that RUNX3 mRNA levels were significantly downregulated in gastric cancer tissues compared with their matched normal tissues, and were closely associated with miR-148a expression. After treatment of human gastric cancer AGS and BGC-823 cells with the DNA methylation inhibitor 5-aza-2’-deoxycytidine, a significant increase in RUNX3 mRNA, RUNX3 protein, and the non-methylated form of the RUNX3 promoter were observed relative to untreated cells. Enforced expression of miR-148a, which can modulate DNMT1 and DNMT3B, also increased the expression of RUNX3 in gastric cancer cells. Knockdown of DNMT1 was associated with increased levels of RUNX3 mRNA and RUNX3 protein, while knockdown of DNMT3B did not have any effect on these in BGC-823 cells. Our results show that miR-148a may regulate RUNX3 expression through modulation of DNMT1-dependent DNA methylation in gastric cancer and highlight a miRNA-epigenetics regulation mechanism of gene expression.
Shim, Junbo,Kim, Min-Kyu,Hong, Seong-Kwan,Kwon, Oh-Kyong IEEE 2018 IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS PART 2 E Vol.65 No.12
<P>This brief presents a 16-bit amplifier-free second-order incremental analog-to-digital converter (IADC2) for sensor applications. The proposed IADC2 employs a power efficient successive approximation register (SAR)-based integrator for the charge transfer operation, which consumes dynamic power only, instead of using power-consuming operational transconductance amplifiers that consume a high static current. The proposed amplifier thus achieves ultra-low-power consumption in low-frequency operation. In addition, the charge redistribution period of a capacitor digital-to-analog converter (CDAC) in the SAR-based integrator is split in a time-interleaving way, such that the CDAC can be shared by the first and second integrators. A test chip, including the proposed IADC2, was fabricated using 0.18- <TEX>${\mu }\text{m}$</TEX> standard CMOS process technology. The measurement results show that the proposed IADC2 achieves a differential nonlinearity of −0.51/+0.74 LSB and an integral nonlinearity of −3.12/+0.24$ LSB. In addition, the measured maximum signal-to-noise ratio, and an effective number of bits are 93.4 dB and 15.22-bit for the dc signal, respectively. The measured power consumption is 0.24 <TEX>${\mu }\text{W}$</TEX> at a sampling frequency of 10 kHz. Therefore, the proposed IADC2 is suitable for various sensor applications requiring ultra-low-power consumption.</P>
Risk Factors for Recurrent Colorectal Polyps
Yuanzhen Hao,Yining Wang,Miao Qi,Xin He,Ying Zhu,Junbo Hong 거트앤리버 소화기연관학회협의회 2020 Gut and Liver Vol.14 No.4
The recurrence of colorectal polyps is caused by various factors and leads to the carcinogenesis of colorectal cancer, which ranks third in incidence and fourth in mortality among cancers worldwide. The potential risk factors for colorectal polyp recurrence have been demonstrated in multiple trials. However, an article that pools and summarizes the various results is needed. This review enumerates and analyzes some risk factors in terms of patient characteristics, procedural operations, polyp characteristics, and dietary aspects to propose some effective prophylactic measures. This review aimed to provide a reference for clinical application and guide patients to prevent colorectal polyp recurrence in a more effective manner.